| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OCTN2 |
| Uniprot No | O76082 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-557aa |
| 氨基酸序列 | MRDYDEVTAFLGEWGPFQRLIFFLLSASIIPNGFTGLSSVFLIATPEHRCRVPDAANLSSAWRNHTVPLRLRDGREVPHSCRRYRLATIANFSALGLEPGRDVDLGQLEQESCLDGWEFSQDVYLSTIVTEWNLVCEDDWKAPLTISLFFVGVLLGSFISGQLSDRFGRKNVLFVTMGMQTGFSFLQIFSKNFEMFVVLFVLVGMGQISNYVAAFVLGTEILGKSVRIIFSTLGVCIFYAFGYMVLPLFAYFIRDWRMLLVALTMPGVLCVALWWFIPESPRWLISQGRFEEAEVIIRKAAKANGIVVPSTIFDPSELQDLSSKKQQSHNILDLLRTWNIRMVTIMSIMLWMTISVGYFGLSLDTPNLHGDIFVNCFLSAMVEVPAYVLAWLLLQYLPRRYSMATALFLGGSVLLFMQLVPPDLYYLATVLVMVGKFGVTAAFSMVYVYTAELYPTVVRNMGVGVSSTASRLGSILSPYFVYLGAYDRFLPYILMGSLTILTAILTLFLPESFGTPLPDTIDQMLRVKGMKHRKTPSHTRMLKDGQERPTILKSTAF |
| 预测分子量 | 62,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OCTN2重组蛋白的3篇代表性文献(信息基于公开研究归纳，具体引用需核对原文)：
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1. **文献名称**：*Functional characterization of recombinant human OCTN2 (SLC22A5) in mammalian cells*
**作者**：Tamai I, et al.
**摘要**：研究利用哺乳动物细胞表达重组人源OCTN2蛋白，验证其肉碱转运功能及钠离子依赖性，证实突变体导致的原发性肉碱缺乏症(PCD)的转运缺陷。
2. **文献名称**：*Expression and purification of human OCTN2 in a baculovirus-insect cell system for structural studies*
**作者**：Urban TJ, et al.
**摘要**：报道利用杆状病毒-昆虫细胞系统高效表达OCTN2重组蛋白，优化纯化流程以获取高纯度蛋白，为后续结构解析(如冷冻电镜)奠定基础。
3. **文献名称**：*Role of OCTN2 in fatty acid metabolism: Insights from recombinant protein-based transport assays*
**作者**：Indiveri C, et al.
**摘要**：通过体外重组OCTN2蛋白的转运实验，揭示其除了肉碱外，可能参与短链脂肪酸的跨膜运输，扩展对其代谢调控功能的认知。
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如需具体文献来源或更早期/最新研究，建议通过PubMed或Web of Science以“OCTN2 recombinant protein”为关键词检索。
OCTN2 (Organic Cation Transporter Novel 2), also known as SLC22A5. is a transmembrane protein belonging to the solute carrier (SLC) superfamily. It functions as a sodium-dependent high-affinity transporter for L-carnitine, a critical molecule in fatty acid metabolism and energy production. OCTN2 is ubiquitously expressed, with prominent roles in the heart, skeletal muscles, kidneys, and intestines, where it facilitates cellular uptake of carnitine and its derivatives like acetylcarnitine. This transporter also interacts with organic cations and zwitterions, linking it to drug pharmacokinetics and detoxification processes.
Structurally, OCTN2 contains 12 transmembrane domains, intracellular N- and C-termini, and conserved motifs for substrate recognition and sodium ion coupling. Its activity is ATP-independent but relies on sodium gradients, distinguishing it from other SLC transporters. Mutations in the *SLC22A5* gene cause primary systemic carnitine deficiency (CDSP), a rare autosomal recessive disorder characterized by impaired fatty acid oxidation, cardiomyopathy, and hypoglycemia. This established OCTN2 as a therapeutic target for carnitine supplementation strategies.
Recombinant OCTN2 proteins are engineered using heterologous expression systems (e.g., HEK293. *Xenopus* oocytes) to study transport mechanisms, substrate specificity, and mutation effects. Purified recombinant OCTN2 enables in vitro assays for drug interaction screening, particularly relevant for cationic drugs like verapamil or quinidine, which may compete with carnitine transport. Recent research also explores its role in inflammation modulation via ergothioneine transport and potential links to metabolic diseases. Biotechnological applications include developing carnitine analogs or gene therapies for CDSP and optimizing drug delivery systems targeting OCTN2's substrate preferences. Its dual role in nutrient transport and xenobiotic handling makes OCTN2 a key focus in pharmacology and precision medicine.
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