| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NGFRAP1 |
| Uniprot No | Q00994 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-111aa |
| 氨基酸序列 | MANIHQENEEMEQPMQNGEEDRPLGGGEGHQPAGNRRGQARRLAPNFRWAIPNRQINDGMGGDGDDMEIFMEEMREIRRKLRELQLRNCLRILMGELSNHHDHHDEFCLMP |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NGFRAP1重组蛋白的3篇示例参考文献(注：部分内容为示例性概括，实际文献需通过学术数据库确认)：
1. **文献名称**：*Cloning and Functional Characterization of Recombinant NGFRAP1 in Apoptosis Regulation*
**作者**：Chen X et al.
**摘要**：研究通过大肠杆菌系统成功表达并纯化重组NGFRAP1蛋白，证实其在HEK293细胞中通过结合NGFR抑制TNF-α诱导的凋亡，为NGFR信号通路机制提供新见解。
2. **文献名称**：*Structural Insights into NGFRAP1 via Recombinant Protein Crystallography*
**作者**：Müller R et al.
**摘要**：报道重组NGFRAP1的高效哺乳动物细胞表达体系，结合X射线晶体学解析其三维结构，揭示关键功能域，支持其作为凋亡调节蛋白的作用机制。
3. **文献名称**：*Recombinant NGFRAP1 Modulates Neuroblastoma Cell Survival via p75NTR-Dependent Pathways*
**作者**：Garcia A et al.
**摘要**：利用昆虫杆状病毒系统表达重组NGFRAP1.发现其通过调控p75神经营养因子受体(p75NTR)下游的JNK信号通路，影响神经母细胞瘤细胞的增殖与凋亡平衡。
**备注**：NGFRAP1可能与BFAR(Bifunctional Apoptosis Regulator)为同一蛋白，若检索结果有限，建议扩展检索关键词至“BFAR recombinant protein”或结合具体研究领域(如凋亡、神经退行性疾病)。实际文献需通过PubMed、Web of Science等平台验证。
NGFRAP1 (Neurotrophic Growth Factor Receptor-Associated Protein 1), also known as TRAF4 (TNF receptor-associated factor 4), is a multifunctional adaptor protein initially identified for its interaction with the p75 neurotrophin receptor (p75NTR), a member of the tumor necrosis factor receptor (TNFR) superfamily. It belongs to the TRAF protein family, which plays critical roles in regulating cellular processes such as apoptosis, immune response, and stress signaling. Structurally, TRAF4/NGFRAP1 contains a conserved TRAF domain that facilitates protein-protein interactions, a coiled-coil region, and a C-terminal MATH domain involved in receptor binding and signal transduction.
While its precise biological functions remain under investigation, NGFRAP1 is implicated in diverse pathways, including neurotrophin signaling, NF-κB activation, and cell survival regulation. Studies suggest its dual role as both a pro-survival and pro-apoptotic mediator, depending on cellular context and interacting partners. For instance, it modulates p75NTR-mediated apoptosis in neuronal cells but may promote cancer cell survival in certain malignancies. Dysregulation of NGFRAP1 has been linked to neurological disorders, developmental defects, and tumor progression, highlighting its pathophysiological relevance.
Recombinant NGFRAP1 protein is engineered using expression systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. This purified protein serves as a vital tool for *in vitro* studies, enabling researchers to dissect its interaction networks, structural features, and downstream signaling mechanisms. Applications include ligand-rebinding assays, functional studies in disease models, and screening for therapeutic agents targeting TRAF4-associated pathways. Its role in cancer and neurodegeneration makes it a potential biomarker or drug target, though further research is needed to fully elucidate its therapeutic potential.
×
