| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CNTNAP3 |
| Uniprot No | Q9BZ76 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1288aa |
| 氨基酸序列 | MASVAWAVLKVLLLLPTQTWSPVGAGNPPDCDAPLASALPRSSFSSSSELSSSHGPGFSRLNRRDGAGGWTPLVSNKYQWLQIDLGERMEVTAVATQGGYGSSDWVTSYLLMFSDGGRNWKQYRREESIWGFPGNTNADSVVHYRLQPPFEARFLRFLPLAWNPRGRIGMRIEVYGCAYKSEVVYFDGQSALLYRLDKKPLKPIRDVISLKFKAMQSNGILLHREGQHGNHITLELIKGKLVFFLNSGNAKLPSTIAPVTLTLGSLLDDQHWHSVLIELLDTQVNFTVDKHTHHFQAKGDSSYLDLNFEISFGGIPTPGRSRAFRRKSFHGCLENLYYNGVDVTELAKKHKPQILMMGNVSFSCPQPQTVPVTFLSSRSYLALPGNSGEDKVSVTFQFRTWNRAGHLLFGELRRGSGSFVLFLKDGKLKLSLFQPGQSPRNVTAGAGLNDGQWHSVSFSAKWSHMNVVVDDDTAVQPLVAVLIDSGDTYYFGGCLDNSSGSGCKSPLGGFQGCLRLITIGDKAVDPILVQQGALGSFRDLQIDSCGITDRCLPSYCEHGGECSQSWDTFSCDCLGTGYTGETCHSSLYEQSCEAHKHRGNPSGLYYIDADGSGPLGPFLVYCNMTADAAWTVVQHGGPDAVTLRGAPSGHPRSAVSFAYAAGAGQLRSAVNLAERCEQRLALRCGTARRPDSRDGTPLSWWVGRTNETHTSWGGSLPDAQKCTCGLEGNCIDSQYYCNCDAGRNEWTSDTIVLSQKEHLPVTQIVMTDAGRPHSEAAYTLGPLLCRGDQSFWNSASFNTETSYLHFPAFHGELTADVCFFFKTTVSSGVFMENLGITDFIRIELRAPTEVTFSFDVGNGPCEVTVQSPTPFNDNQWHHVRAERNVKGASLQVDQLPQKMQPAPADGHVRLQLNSQLFIGGTATRQRGFLGCIRSLQLNGVALDLEERATVTPGVEPGCAGHCSTYGHLCRNGGRCREKRRGVTCDCAFSAYDGPFCSNEISAYFATGSSMTYHFQEHYTLSENSSSLVSSLHRDVTLTREMITLSFRTTRTPSLLLYVSSFYEEYLSVILANNGSLQIRYKLDRHQNPDAFTFDFKNMADGQLHQVKINREEAVVMVEVNQSTKKQVILSSGTEFNAVKSLILGKVLEAAGADPDTRRAATSGFTGCLSAVRFGRAAPLKAALRPSGPSRVTVRGHVAPMARCAAGAASGSPARELAPRLAGGAGRSGPADEGEPLVNADRRDSAVIGGVIAVVIFILLCITAIAIRIYQQRKLRKENESKVSKKEEC |
| 预测分子量 | 140 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CNTNAP3重组蛋白的3篇参考文献及其摘要内容的示例(注：以下文献为虚拟示例，实际引用时需核实真实来源)：
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1. **文献名称**: *"Recombinant CNTNAP3 Protein Modulates Neuronal Adhesion and Synaptic Plasticity in vitro"*
**作者**: Thompson R., et al.
**摘要**: 本研究通过大肠杆菌表达系统成功纯化了重组CNTNAP3蛋白，并发现其能够显著增强原代培养神经元的细胞间粘附能力。进一步实验表明，CNTNAP3重组蛋白通过调节突触后致密蛋白(如PSD-95)的聚集，影响突触可塑性，提示其在神经发育障碍中的潜在作用。
2. **文献名称**: *"Functional Characterization of CNTNAP3 Extracellular Domain: Implications for Autoimmune Disorders"*
**作者**: García-Sánchez M., et al.
**摘要**: 作者利用哺乳动物细胞系(HEK293)表达了带有His标签的重组CNTNAP3胞外域蛋白，并通过表面等离子共振(SPR)分析其与自身抗体间的相互作用。结果显示，CNTNAP3重组蛋白可特异性结合类风湿性关节炎患者血清中的自身抗体，提示其可能作为自身免疫疾病的生物标志物。
3. **文献名称**: *"CNTNAP3 Recombinant Protein Enhances Cancer Cell Invasion via Metalloproteinase Activation"*
**作者**: Chen L., et al.
**摘要**: 该研究在昆虫细胞中表达并纯化了全长CNTNAP3重组蛋白，发现其可激活基质金属蛋白酶(MMP-9)的表达，促进乳腺癌细胞的侵袭和迁移。机制研究表明，CNTNAP3通过Wnt/β-catenin信号通路发挥作用，为靶向CNTNAP3的癌症治疗提供了新思路。
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**说明**：
- 实际研究中，CNTNAP3重组蛋白的文献可能较少，建议结合具体研究领域(如神经科学、癌症或免疫学)扩大检索范围。
- 可通过PubMed、Google Scholar等平台以关键词“CNTNAP3 recombinant protein”或“CNTNAP3 expression and function”检索真实文献。
- 若需具体文献指导，可进一步提供研究背景或目标。
The CNTNAP3 (Contactin-associated protein-like 3) gene encodes a member of the neurexin superfamily, a group of transmembrane proteins implicated in cell adhesion, synaptic organization, and nervous system development. As part of the larger CNTNAP family, CNTNAP3 shares structural homology with CASPR proteins, characterized by extracellular domains such as laminin G, epidermal growth factor (EGF)-like repeats, and fibrinogen-like regions, which mediate protein-protein interactions. While its paralog CNTNAP2 is extensively studied for roles in neurodevelopmental disorders like autism, CNTNAP3 remains less characterized, though emerging evidence suggests involvement in cellular communication, axon guidance, and possibly tumorigenesis.
Recombinant CNTNAP3 protein is engineered to study its molecular functions, often expressed in vitro using systems like mammalian or insect cell cultures to ensure proper post-translational modifications. Its recombinant form enables investigations into binding partners, signaling pathways, and structural features. Studies indicate CNTNAP3 may interact with contactins or extracellular matrix components, influencing cell adhesion and migration. Dysregulation of CNTNAP3 has been tentatively linked to cancers, including hepatocellular carcinoma and glioblastoma, where it may modulate metastasis or proliferation pathways.
Research on recombinant CNTNAP3 also explores its diagnostic or therapeutic potential. For example, antibodies generated against recombinant CNTNAP3 are used to detect expression patterns in tissues, revealing elevated levels in certain malignancies. Additionally, structural analyses (e.g., X-ray crystallography or cryo-EM) of the recombinant protein aim to resolve domain-specific mechanisms, aiding drug design. Despite progress, CNTNAP3’s precise physiological roles and pathological relevance require further elucidation, positioning recombinant protein tools as critical assets for bridging molecular insights with clinical applications.
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