| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDC73 |
| Uniprot No | Q6P1J9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-531aa |
| 氨基酸序列 | ADVLSVLRQ YNIQKKEIVV KGDEVIFGEF SWPKNVKTNY VVWGTGKEGQ PREYYTLDSI LFLLNNVHLS HPVYVRRAAT ENIPVVRRPD RKDLLGYLNG EASTSASIDR SAPLEIGLQR STQVKRAADE VLAEAKKPRI EDEECVRLDK ERLAARLEGH KEGIVQTEQI RSLSEAMSVE KIAAIKAKIM AKKRSTIKTD LDDDITALKQ RSFVDAEVDV TRDIVSRERV WRTRTTILQS TGKNFSKNIF AILQSVKARE EGRAPEQRPA PNAAPVDPTL RTKQPIPAAY NRYDQERFKG KEETEGFKID TMGTYHGMTL KSVTEGASAR KTQTPAAQPV PRPVSQARPP PNQKKGSRTP IIIIPAATTS LITMLNAKDL LQDLKFVPSD EKKKQGCQRE NETLIQRRKD QMQPGGTAIS VTVPYRVVDQ PLKLMPQDWD RVVAVFVQGP AWQFKGWPWL LPDGSPVDIF AKIKAFHLKY DEVRLDPNVQ KWDVTVLELS YHKRHLDRPV FLRFWETLDR YMVKHKSHLR F |
| 预测分子量 | 60,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDC73重组蛋白的3篇参考文献的简要概括(注：文献信息为模拟示例，实际引用请核实原始文献)：
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1. **文献名称**：*Recombinant Parafibromin Inhibits β-Catenin Signaling and Suppresses Tumor Growth*
**作者**：Tan M.H., et al.
**摘要**：研究利用重组表达的parafibromin蛋白(CDC73编码产物)，验证其通过结合β-catenin抑制Wnt信号通路的分子机制，并证明其在体外抑制癌细胞增殖的能力。
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2. **文献名称**：*Structural Insights into CDC73 Protein Stability and Interaction with PAF1 Complex*
**作者**：Wang J., et al.
**摘要**：通过重组CDC73蛋白的晶体结构解析，揭示了其N端结构域对PAF1转录复合物结合的关键作用，并发现特定突变导致蛋白稳定性下降与癌症发生相关。
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3. **文献名称**：*Functional Characterization of CDC73 Mutations in Parathyroid Carcinoma Using Recombinant Protein Models*
**作者**：Howell V.M., et al.
**摘要**：构建携带甲状旁腺癌常见CDC73突变的重组蛋白，发现突变体丧失与RNA聚合酶II的结合能力，导致转录调控异常，为靶向治疗提供依据。
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如需具体文献，建议通过PubMed或Google Scholar搜索关键词 **CDC73 recombinant protein**、**parafibromin expression** 或 **HRPT2 functional studies** 获取最新研究。
**Background of CDC73 Recombinant Protein**
The CDC73 gene (Cell Division Cycle 73), also known as *HRPT2*, encodes a tumor suppressor protein called parafibromin. This protein plays a critical role in transcriptional regulation, cell cycle control, and histone modification as part of the PAF1 complex, which interacts with RNA polymerase II to modulate gene expression. CDC73 mutations are strongly linked to hyperparathyroidism-jaw tumor (HPT-JT) syndrome, familial isolated hyperparathyroidism, and sporadic parathyroid carcinomas, highlighting its importance in preventing tumorigenesis.
Recombinant CDC73 protein is produced in vitro using expression systems (e.g., *E. coli* or mammalian cells) to study its structure, biochemical interactions, and functional roles. Researchers utilize this engineered protein to investigate how pathogenic mutations (e.g., frameshift, nonsense) disrupt its tumor-suppressive functions, such as impairing binding to β-catenin or compromising its role in Wnt signaling. Additionally, recombinant CDC73 aids in exploring its involvement in epigenetic regulation, including histone H3K4 methylation and ubiquitination processes.
In clinical contexts, recombinant CDC73 serves as a tool for developing diagnostic assays or therapeutic strategies targeting CDC73-deficient cancers. Studies also focus on its interaction with components of the PAF1 complex and other partners like CUL4A-DDB1 ubiquitin ligase, shedding light on pathways dysregulated in tumors. The protein’s conserved C-terminal region, critical for nuclear localization and function, is a focal point for functional analyses.
Overall, CDC73 recombinant protein is pivotal for advancing molecular insights into endocrine tumors and developing precision medicine approaches for CDC73-related disorders.
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