| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CBLN2 |
| Uniprot No | Q8IUK8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 52-224aa |
| 氨基酸序列 | QNDTEPIVL EGKCLVVCDS SPSADGAVTS SLGISVRSGS AKVAFSATRS TNHEPSEMSN RTMTIYFDQV LVNIGNHFDL ASSIFVAPRK GIYSFSFHVV KVYNRQTIQV SLMQNGYPVI SAFAGDQDVT REAASNGVLL LMEREDKVHL KLERGNLMGG WKYSTFSGFL VFPL |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and functional analysis of Cbln2 reveals its role in synaptogenesis"**
- Author: Li, Y. et al.
- 摘要：研究报道了重组CBLN2蛋白的晶体结构，揭示了其通过结合突触前神经毡蛋白(neurexin)和突触后谷氨酸受体，促进兴奋性突触形成的分子机制。
2. **"Recombinant CBLN2 modulates synaptic plasticity in hippocampal neurons"**
- Author: Watanabe, M. et al.
- 摘要：通过体外实验证明，重组CBLN2蛋白能够增强海马神经元的突触可塑性，并依赖突触后GluD2受体的信号通路，提示其在记忆相关神经环路中的作用。
3. **"Expression and purification of biologically active Cbln2 in mammalian cells"**
- Author: Zhang, X. et al.
- 摘要：优化了重组CBLN2蛋白在哺乳动物细胞中的表达与纯化方法，验证其与配体结合活性，为后续功能研究提供高纯度工具蛋白。
4. **"Cbln2 deficiency and recombinant protein rescue in autism-related behaviors"**
- Author: Sclip, A. et al.
- 摘要：在自闭症模型小鼠中，重组CBLN2蛋白可逆转因基因缺失导致的社交行为异常和突触连接缺陷，提示其治疗神经发育障碍的潜力。
(注：以上文献信息为模拟示例，实际文献需通过学术数据库检索确认。)
Cerebellin-2 (CBLN2) is a member of the cerebellin protein family, which comprises secreted glycoproteins predominantly expressed in the brain. Initially identified as a cerebellum-enriched protein, CBLN2 is now recognized to have broader expression patterns, including the cerebral cortex, hippocampus, and olfactory bulb. It plays a critical role in synaptic organization and neurotransmission by functioning as a transneuronal adhesion molecule. Structurally, CBLN2 contains a conserved N-terminal cerebellin domain and a C-terminal hexa-disulfide-core motif, enabling interactions with presynaptic neurexins and postsynaptic glutamate receptors (e.g., GluD2). These interactions facilitate the formation and maintenance of excitatory synapses, particularly in regions associated with cognitive and sensory processing.
Recombinant CBLN2 protein is engineered using heterologous expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications, such as glycosylation and disulfide bond formation. This allows researchers to study its biochemical properties, receptor-binding specificity, and functional roles in vitro and in vivo. Studies using recombinant CBLN2 have highlighted its involvement in synaptic plasticity, neural circuit assembly, and behaviors linked to learning and memory. Dysregulation of CBLN2 has been implicated in neurodevelopmental and psychiatric disorders, including autism spectrum disorders and schizophrenia, making it a potential therapeutic target. Additionally, recombinant CBLN2 serves as a tool to dissect molecular mechanisms underlying synapse diversity and neural network connectivity, advancing our understanding of brain function and pathology.
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