| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ERCC8 |
| Uniprot No | Q13216 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-396aa |
| 氨基酸序列 | MLGFLSARQT GLEDPLRLRR AESTRRVLGL ELNKDRDVER IHGGGINTLD IEPVEGRYML SGGSDGVIVL YDLENSSRQS YYTCKAVCSI GRDHPDVHRY SVETVQWYPH DTGMFTSSSF DKTLKVWDTN TLQTADVFNF EETVYSHHMS PVSTKHCLVA VGTRGPKVQL CDLKSGSCSH ILQGHRQEIL AVSWSPRYDY ILATASADSR VKLWDVRRAS GCLITLDQHN GKKSQAVESA NTAHNGKVNG LCFTSDGLHL LTVGTDNRMR LWNSSNGENT LVNYGKVCNN SKKGLKFTVS CGCSSEFVFV PYGSTIAVYT VYSGEQITML KGHYKTVDCC VFQSNFQELY SGSRDCNILA WVPSLYEPVP DDDETTTKSQ LNPAFEDAWS SSDEEG |
| 预测分子量 | 44 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ERCC8重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "Recombinant ERCC8 (CSA) Protein Restores DNA Repair Capacity in Cockayne Syndrome Cells"
**作者**: Laugel V. et al.
**摘要**: 研究通过表达重组ERCC8蛋白，验证其在Cockayne综合征患者细胞中修复紫外线诱导DNA损伤的能力，发现其与ERCC6(CSB)协同作用，恢复转录偶联修复功能。
2. **文献名称**: "Structural and Functional Analysis of the ERCC8-DDB1 Complex in Nucleotide Excision Repair"
**作者**: Fischer E.S. et al.
**摘要**: 利用重组ERCC8和DDB1蛋白解析复合物晶体结构，揭示ERCC8通过结合DDB1参与损伤识别，并调控泛素连接酶活性，对修复紫外线损伤至关重要。
3. **文献名称**: "Expression and Purification of Functional ERCC8 Protein in a Baculovirus System"
**作者**: Kitao S. et al.
**摘要**: 报道了利用杆状病毒系统高效表达重组ERCC8蛋白的方法，纯化后蛋白在体外实验中验证了其与DNA损伤位点的结合活性及修复功能。
The ERCC8 (Excision Repair Cross-Complementation Group 8) protein, also known as CSA, plays a critical role in DNA repair mechanisms, specifically in the transcription-coupled nucleotide excision repair (TC-NER) pathway. This pathway targets DNA lesions, such as UV-induced cyclobutane pyrimidine dimers or chemical adducts, that block RNA polymerase II during transcription. ERCC8 forms a complex with other proteins, including ERCC6 (CSB), to recognize stalled transcription machinery and recruit downstream repair factors. Mutations in the ERCC8 gene are linked to Cockayne syndrome (CS), a rare autosomal recessive disorder characterized by growth failure, premature aging, neurological dysfunction, and photosensitivity. CS is categorized into complementation groups, with ERCC8 mutations primarily associated with complementation group A (CS-A).
Recombinant ERCC8 protein is produced using biotechnological systems (e.g., bacterial, yeast, or mammalian expression) to enable functional and structural studies. Its production involves cloning the ERCC8 gene into expression vectors, followed by purification via affinity chromatography. Recombinant ERCC8 is essential for elucidating its interaction networks, repair mechanisms, and disease-related mutations. Researchers use it to reconstitute repair pathways in vitro, analyze binding partners, and test potential therapeutic compounds. Additionally, it aids in studying the molecular basis of CS and developing gene therapies or protein replacement strategies. By understanding ERCC8's role in TC-NER, insights into broader DNA repair deficiencies, cancer susceptibility, and aging-related pathologies can be advanced.
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