| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R7 |
| Uniprot No | Q15435 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-360aa |
| 氨基酸序列 | AAERGAGQQ QSQEMMEVDR RVESEESGDE EGKKHSSGIV ADLSEQSLKD GEERGEEDPE EEHELPVDME TINLDRDAED VDLNHYRIGK IEGFEVLKKV KTLCLRQNLI KCIENLEELQ SLRELDLYDN QIKKIENLEA LTELEILDIS FNLLRNIEGV DKLTRLKKLF LVNNKISKIE NLSNLHQLQM LELGSNRIRA IENIDTLTNL ESLFLGKNKI TKLQNLDALT NLTVLSMQSN RLTKIEGLQN LVNLRELYLS HNGIEVIEGL ENNNKLTMLD IASNRIKKIE NISHLTELQE FWMNDNLLES WSDLDELKGA RSLETVYLER NPLQKDPQYR RKVMLALPSV RQIDATFVRF |
| 预测分子量 | 41,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1R7重组蛋白的假设性参考文献示例，供参考：
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1. **标题**：*PPP1R7 regulates mitotic exit through interaction with Protein Phosphatase 1*
**作者**：Smith A, et al.
**摘要**：研究揭示了PPP1R7作为PP1的调控亚基，通过重组蛋白实验证实其参与有丝分裂退出的磷酸酶活性调控，并解析了其关键结构域的作用。
2. **标题**：*Structural characterization of recombinant PPP1R7 and its binding partners*
**作者**：Chen L, et al.
**摘要**：利用重组PPP1R7蛋白进行体外互作实验，鉴定了其与细胞周期相关蛋白的结合界面，并提出了其调控PP1底物特异性的分子机制。
3. **标题**：*PPP1R7 overexpression alters cancer cell proliferation via PP1 signaling*
**作者**：Wang Y, et al.
**摘要**：通过重组PPP1R7在肿瘤细胞中的过表达，发现其通过调节PP1活性抑制细胞增殖，提示其在癌症治疗中的潜在靶点价值。
4. **标题**：*High-yield expression and purification of recombinant PPP1R7 in E. coli*
**作者**：Kim J, et al.
**摘要**：报道了一种高效重组PPP1R7蛋白的原核表达系统，优化了纯化条件，并验证了其体外生物活性，为后续功能研究提供可靠工具。
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**注意**：以上文献为假设性示例，实际研究中请通过学术数据库检索真实文献。
**Background of PPP1R7 Recombinant Protein**
PPP1R7 (Protein Phosphatase 1 Regulatory Subunit 7) is a regulatory component of protein phosphatase 1 (PP1), a serine/threonine phosphatase critical for diverse cellular processes, including cell cycle progression, signal transduction, and metabolic regulation. PPP1R7. also known as SDS22. functions as a conserved PP1-binding partner that modulates the enzyme’s substrate specificity, activity, and subcellular localization. Structurally, PPP1R7 contains multiple leucine-rich repeats (LRRs) that facilitate interactions with PP1. stabilizing its conformation and influencing its recruitment to specific targets.
Recombinant PPP1R7 protein is engineered using expression systems like *E. coli* or mammalian cells, often tagged with affinity markers (e.g., His-tag) for purification. Its production enables mechanistic studies of PP1 regulation, particularly in contexts such as mitosis, where PPP1R7-PP1 complexes are implicated in dephosphorylating key cell cycle proteins (e.g., Aurora kinases). Dysregulation of PPP1R7 has been linked to diseases, including cancers and neurological disorders, highlighting its role in maintaining cellular homeostasis.
Research applications include *in vitro* phosphatase assays, structural studies to elucidate PP1 regulatory mechanisms, and screening for therapeutic agents targeting PP1-associated pathways. PPP1R7 recombinant protein also serves as a tool to explore its potential as a biomarker or drug target in diseases linked to PP1 dysfunction.
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