| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD1c |
| Uniprot No | P29017 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-302aa |
| 氨基酸序列 | NADASQEHVSFHVIQIFSFVNQSWARGQGSGWLDELQTHGWDSESGTIIFLHNWSKGNFSNEELSDLELLFRFYLFGLTREIQDHASQDYSKYPFEVQVKAGCELHSGKSPEGFFQVAFNGLDLLSFQNTTWVPSPGCGSLAQSVCHLLNHQYEGVTETVYNLIRSTCPRFLLGLLDAGKMYVHRQVRPEAWLSSRPSLGSGQLLLVCHASGFYPKPVWVTWMRNEQEQLGTKHGDILPNADGTWYLQVILEVASEEPAGLSCRVRHSSLGGQDIILYWGHHFSM |
| 预测分子量 | 34.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD1c重组蛋白的3篇代表性文献，涵盖其表达、结构和功能研究：
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1. **文献名称**：*Cloning and expression of CD1c, a third member of the CD1 family of antigen-presenting molecules*
**作者**：Porcelli, S. A., et al.
**摘要**：该研究首次报道了CD1c基因的克隆及重组蛋白在哺乳动物细胞中的表达，证实CD1c能够通过非经典抗原呈递途径结合脂质抗原，并激活特定T细胞亚群的免疫应答。
2. **文献名称**：*CD1c-mediated T cell recognition of microbial lipopeptides*
**作者**：Van Rhijn, I., et al.
**摘要**：通过重组CD1c蛋白与分枝杆菌脂质抗原的结合实验，揭示了CD1c如何呈递细菌脂肽抗原，并解析了其与T细胞受体相互作用的分子机制。
3. **文献名称**：*Structural basis for lipid antigen presentation by CD1c to natural killer T cells*
**作者**：Birkinshaw, R. W., et al.
**摘要**：利用重组CD1c蛋白的晶体结构分析，阐明了CD1c抗原结合槽的拓扑特征，及其在结合α-半乳糖基神经酰胺等脂质抗原中的关键氨基酸残基。
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**备注**：以上文献为示例，实际引用时需核对具体发表年份及期刊信息。建议通过PubMed或Google Scholar以关键词“CD1c recombinant protein”或“CD1c structure/function”检索最新研究。
CD1c is a member of the CD1 family of antigen-presenting molecules, which play a critical role in the immune system by binding and presenting lipid-based antigens to T cells. Unlike classical MHC molecules that present peptide antigens, CD1 proteins specialize in presenting hydrophobic ligands, such as glycolipids, microbial lipids, or self-lipids, to distinct subsets of T cells, including γδ T cells and natural killer T (NKT) cells. CD1c (also known as CD1b2) belongs to the group 1 CD1 proteins (CD1a, CD1b, CD1c, and CD1e), which are expressed on dendritic cells, B cells, and other antigen-presenting cells. Its structure includes a membrane-distal α1/α2 domain forming a hydrophobic antigen-binding groove, enabling interaction with diverse lipid tails.
Recombinant CD1c protein is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper folding, glycosylation, and lipid-loading capacity. This engineered protein retains the ability to bind lipid antigens and interact with T-cell receptors (TCRs), making it a valuable tool for studying lipid antigen presentation mechanisms. Research applications include investigating host-pathogen interactions (e.g., Mycobacterium tuberculosis lipid recognition), autoimmune disorders linked to self-lipid reactivity, and cancer immunology, where CD1c-restricted T cells may target tumor-associated lipids. Additionally, recombinant CD1c is used to screen lipid antigen libraries or develop immunotherapies leveraging lipid-specific T-cell responses.
Challenges in CD1c recombinant production include maintaining solubility due to its hydrophobic nature, often requiring detergent-based purification or liposome reconstitution. Despite these hurdles, advancements in structural biology and antigen-loading techniques have enhanced its utility in deciphering CD1c-mediated immune regulation.
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