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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CKLF |
| Uniprot No | Q9UBR5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-152aa |
| 氨基酸序列 | MDNVQPKIKHRPFCFSVKGHVKMLRLALTVTSMTFFIIAQAPEPYIVITGFEVTVILFFILLYVLRLDRLMKWLFWPLLDIINSLVTTVFMLIVSVLALIPETTTLTVGGGVFALVTAVCCLADGALIYRKLLFNPSGPYQKKPVHEKKEVL |
| 预测分子量 | 17,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CKLF(Chemokine-like factor)重组蛋白的3篇参考文献概览：
1. **《Cloning, expression, and functional characterization of CKLF1 and its role in immune regulation》**
- 作者：Li, X., et al.
- 摘要：研究通过重组技术在大肠杆菌中表达CKLF1蛋白，验证其在趋化单核细胞和T细胞迁移中的作用，并揭示其通过CCR4受体介导免疫反应的机制。
2. **《Recombinant CKLF2 promotes angiogenesis and endothelial cell proliferation via PI3K/Akt pathway》**
- 作者：Wang, Y., et al.
- 摘要：利用哺乳动物细胞系统表达CKLF2重组蛋白，发现其通过激活PI3K/Akt信号通路促进血管内皮细胞增殖和血管生成，提示其在缺血性疾病中的潜在治疗价值。
3. **《CKLF1 exacerbates neuroinflammation in Alzheimer's disease model via NLRP3 inflammasome activation》**
- 作者：Zhang, H., et al.
- 摘要：通过重组CKLF1蛋白处理小鼠神经胶质细胞，证实其通过激活NLRP3炎症小体加重神经炎症，为阿尔茨海默病的病理机制提供了新视角。
注：以上文献信息为模拟示例，实际研究需查阅具体数据库(如PubMed/Google Scholar)获取真实文献。
**Background of CKLF Recombinant Protein**
Chemokine-like factor (CKLF) is a small secretory protein belonging to the chemokine family, first identified in 2001. It shares structural homology with chemokines, particularly CC-chemokines, and plays roles in immune regulation, inflammation, and cellular migration. CKLF is encoded by the *CKLF* gene located on human chromosome 16 and exists in multiple isoforms due to alternative splicing, with CKLF1 being the most studied. It interacts with chemokine receptors, such as CCR4 and CCR5. and activates downstream signaling pathways like MAPK and NF-κB, influencing leukocyte trafficking and tissue remodeling.
CKLF is implicated in diverse pathological processes, including asthma, cancer progression, autoimmune diseases, and neurodegenerative disorders. Its overexpression correlates with tumor metastasis, angiogenesis, and chronic inflammation, making it a potential therapeutic target. To study its functions, recombinant CKLF proteins are produced using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems, ensuring proper folding and post-translational modifications. These proteins are purified via affinity chromatography and validated for bioactivity in cell migration assays or receptor-binding studies.
Research on CKLF recombinant proteins focuses on elucidating its interaction networks, developing neutralizing antibodies, and exploring therapeutic applications. For example, inhibitors targeting CKLF or its receptors are being tested in preclinical models for inflammatory diseases and cancer. Additionally, CKLF serves as a biomarker in diagnostic assays to monitor disease progression. Despite progress, challenges remain in understanding its isoform-specific roles and optimizing delivery strategies for clinical translation. Ongoing studies aim to bridge these gaps, leveraging recombinant protein technology to advance CKLF-related biomedical research.
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