| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TSPAN25 |
| Uniprot No | P19397 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-219aa |
| 氨基酸序列 | MGMSSLKLLKYVLFFFNLLFWICGCCILGFGIYLLIHNNFGVLFHNLPSLTLGNVFVIVGSIIMVVAFLGCMGSIKENKCLLMSFFILLLIILLAEVTLAILLFVYEQKLNEYVAKGLTDSIHRYHSDNSTKAAWDSIQSFLQCCGINGTSDWTSGPPASCPSDRKVEGCYAKARLWFHSNFLYIGIITICVCVIEVLGMSFALTLNCQIDKTSQTIGL |
| 预测分子量 | 24,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TSPAN25重组蛋白的模拟参考文献示例(非真实文献，仅供格式参考)：
1. **《TSPAN25重组蛋白调控乳腺癌细胞迁移的分子机制研究》**
- 作者：Smith A, et al.
- 摘要：研究通过重组表达人源TSPAN25蛋白，发现其通过激活PI3K/AKT信号通路促进乳腺癌细胞的迁移和侵袭，为靶向治疗提供潜在靶点。
2. **《TSPAN25重组蛋白在HEK293细胞中的高效表达与纯化》**
- 作者：Zhang L, et al.
- 摘要：开发了一种基于HEK293细胞的重组TSPAN25蛋白表达系统，通过亲和层析获得高纯度蛋白，并验证其与细胞表面受体的结合活性。
3. **《TSPAN25与整合素α6β4的相互作用促进肿瘤细胞黏附》**
- 作者：Brown K, et al.
- 摘要：利用重组TSPAN25蛋白进行互作实验，揭示其通过结合整合素α6β4增强肿瘤细胞在细胞外基质中的黏附能力。
4. **《TSPAN25胞外结构域的晶体结构解析及其功能意义》**
- 作者：Yamamoto T, et al.
- 摘要：首次报道TSPAN25重组蛋白胞外域的X射线晶体结构，揭示其独特的四螺旋束构象，为理解其跨膜信号转导机制提供结构基础。
(注：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science等平台检索真实文献。)
**Background of TSPAN25 Recombinant Protein**
TSPAN25. a member of the tetraspanin family, is a transmembrane protein characterized by four conserved hydrophobic domains that form two extracellular loops and short intracellular termini. This family plays critical roles in cellular processes such as adhesion, migration, signaling, and membrane organization by facilitating protein-protein interactions and forming functional microdomains called "tetraspanin webs." TSPAN25. also known as TSN-1 or NET-11. is less studied compared to other tetraspanins but has been implicated in immune regulation, cancer progression, and viral infection. For instance, it interacts with integrins and modulates cell motility, potentially influencing metastasis.
Recombinant TSPAN25 protein is engineered to enable detailed functional and structural studies. Typically produced in mammalian or insect cell systems using recombinant DNA technology, it retains post-translational modifications essential for its biological activity. The protein is purified via affinity tags (e.g., His-tag) and validated for quality, ensuring its suitability for applications like ligand-rebinding assays, antibody development, or mechanistic studies in cancer biology.
Research on TSPAN25 is expanding due to its potential as a therapeutic target. Its overexpression in certain cancers correlates with poor prognosis, suggesting roles in tumor microenvironment modulation. Additionally, tetraspanins are known to mediate viral entry (e.g., hepatitis C virus), positioning TSPAN25 as a candidate for antiviral research. However, its precise mechanisms remain unclear, necessitating further exploration using recombinant protein-based approaches.
In summary, TSPAN25 recombinant protein serves as a vital tool for unraveling the biological and pathological roles of this understudied tetraspanin, bridging gaps in understanding its contributions to disease and therapy.
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