| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRD |
| Uniprot No | P23468-1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-1265aa |
| 氨基酸序列 | ETPPRFTRTPVDQTGVSGGVASFICQATGDPRPKIVWNKKGKKVSNQRFEVIEFDDGSGSVLRIQPLRTPRDEAIYECVASNNVGEISVSTRLTVLREDQIPRGFPTIDMGPQLKVVERTRTATMLCAASGNPDPEITWFKDFLPVDTSNNNGRIKQLRSESIGGTPIRGALQIEQSEESDQGKYECVATNSAGTRYSAPANLYVRELREVRRVPPRFSIPPTNHEIMPGGSVNITCVAVGSPMPYVKWMLGAEDLTPEDDMPIGRNVLELNDVRQSANYTCVAMSTLGVIEAIAQITVKALPKPPGTPVVTESTATSITLTWDSGNPEPVSYYIIQHKPKNSEELYKEIDGVATTRYSVAGLSPYSDYEFRVVAVNNIGRGPPSEPVLTQTSEQAPSSAPRDVQARMLSSTTILVQWKEPEEPNGQIQGYRVYYTMDPTQHVNNWMKHNVADSQITTIGNLVPQKTYSVKVLAFTSIGDGPLSSDIQVITQTGVPGQPLNFKAEPESETSILLSWTPPRSDTIANYELVYKDGEHGEEQRITIEPGTSYRLQGLKPNSLYYFRLAARSPQGLGASTAEISARTMQSKPSAPPQDISCTSPSSTSILVSWQPPPVEKQNGIITEYSIKYTAVDGEDDKPHEILGIPSDTTKYLLEQLEKWTEYRITVTAHTDVGPGPESLSVLIRTNEDVPSGPPRKVEVEAVNSTSVKVSWRSPVPNKQHGQIRGYQVHYVRMENGEPKGQPMLKDVMLADAQWEFDDTTEHDMIISGLQPETSYSLTVTAYTTKGDGARSKPKLVSTTGAVPGKPRLVINHTQMNTALIQWHPPVDTFGPLQGYRLKFGRKDMEPLTTLEFSEKEDHFTATDIHKGASYVFRLSARNKVGFGEEMVKEISIPEEVPTGFPQNLHSEGTTSTSVQLSWQPPVLAERNGIITKYTLLYRDINIPLLPMEQLIVPADTTMTLTGLKPDTTYDVKVRAHTSKGPGPYSPSVQFRTLPVDQVFAKNFHVKAVMKTSVLLSWEIPENYNSAMPFKILYDDGKMVEEVDGRATQKLIVNLKPEKSYSFVLTNRGNSAGGLQHRVTAKTAPDVLRTKPAFIGKTNLDGMITVQLPEVPANENIKGYYIIIVPLKKSRGKFIKPWESPDEMELDELLKEISRKRRSIRYGREVELKPYIAAHFDVLPTEFTLGDDKHYGGFTNKQLQSGQEYVFFVLAVMEHAESKMYATSPYSDPVVSMDLDPQPITDEEE |
| 预测分子量 | 139.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PTPRD重组蛋白的3篇参考文献及其摘要概括：
1. **标题**：*Structural and functional analysis of the extracellular region of the receptor-type protein tyrosine phosphatase PTPRD*
**作者**：Aricescu AR et al.
**摘要**：该研究解析了PTPRD胞外区域的晶体结构，揭示了其介导细胞粘附的分子机制，并通过重组蛋白实验证明其与配体(如SlitRK)的相互作用对神经轴突导向至关重要。
2. **标题**：*PTPRD mutations promote glioblastoma cell migration and invasion through regulation of STAT3 activity*
**作者**：Wang Z et al.
**摘要**：利用重组PTPRD蛋白进行功能研究，发现其磷酸酶结构域突变会增强STAT3信号通路活性，促进胶质母细胞瘤细胞的迁移和侵袭，提示PTPRD作为肿瘤抑制因子的作用机制。
3. **标题**：*High-throughput screening identifies small molecule inhibitors of PTPRD with potential therapeutic applications in neuropathic pain*
**作者**：Xie Y et al.
**摘要**：通过重组PTPRD蛋白进行高通量药物筛选，发现特异性小分子抑制剂可调控其磷酸酶活性，为神经病理性疼痛和糖尿病相关靶点治疗提供了新策略。
(注：以上文献信息为示例，具体内容需根据实际发表论文调整。建议通过PubMed或Google Scholar以“PTPRD recombinant protein”为关键词检索最新文献。)
The protein tyrosine phosphatase receptor type D (PTPRD) is a member of the receptor-type protein tyrosine phosphatase (RPTP) family, which plays critical roles in regulating cellular signaling pathways involved in cell adhesion, growth, differentiation, and synaptic plasticity. Located on human chromosome 9p, PTPRD encodes a transmembrane protein consisting of an extracellular region with immunoglobulin-like and fibronectin type III domains, a single transmembrane helix, and two intracellular phosphatase domains. Its extracellular domain mediates homophilic or heterophilic interactions with cell adhesion molecules, while the intracellular phosphatase activity modulates tyrosine phosphorylation of downstream targets, including STAT3. ALK, and TrkC, influencing neuronal development and cancer pathways.
PTPRD is highly expressed in the nervous system and is implicated in neurodevelopmental processes, axon guidance, and synapse formation. Dysregulation or mutations in PTPRD have been associated with various cancers, neuropsychiatric disorders (e.g., addiction, ADHD), and metabolic diseases. As a tumor suppressor, it often exhibits genomic deletions or inactivating mutations in glioblastoma, lung cancer, and melanoma, contributing to uncontrolled cell proliferation and metastasis.
Recombinant PTPRD proteins, typically produced in mammalian or insect cell systems to ensure proper post-translational modifications, are essential tools for studying its biochemical activity, ligand interactions, and signaling mechanisms. These proteins enable in vitro assays to screen for phosphatase inhibitors or activators, explore structural-functional relationships, and develop therapeutic strategies targeting PTPRD-related pathologies. Additionally, recombinant forms lacking the transmembrane domain (soluble ectodomains) are used to investigate cell-cell adhesion or as decoy receptors to modulate signaling in disease models. Research on PTPRD recombinant proteins continues to advance understanding of its dual role in neurobiology and oncology, highlighting its potential as a diagnostic marker or therapeutic target.
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