| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DOK4 |
| Uniprot No | Q8TEW6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-326aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMATNFSD IVKQGYVKMK SRKLGIYRRC WLVFRKSSSK GPQRLEKYPD EKSVCLRGCP KVTEISNVKC VTRLPKETKR QAVAIIFTDD SARTFTCDSE LEAEEWYKTL SVECLGSRLN DISLGEPDLL APGVQCEQTD RFNVFLLPCP NLDVYGECKL QITHENIYLW DIHNPRVKLV SWPLCSLRRY GRDATRFTFE AGRMCDAGEG LYTFQTQEGE QIYQRVHSAT LAIAEQHKRV LLEMEKNVRL LNKGTEHYSY PCTPTTMLPR SAYWHHITGS QNIAEASSYA GEGYGAAQAS SETDLLNRFI LLKPKPSQGD SSEAKTPSQ |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOK4重组蛋白的3篇代表性文献摘要概括：
1. **文献名称**：*DOK4 mediates signaling via the RANK receptor in osteoclasts*
**作者**：Yoda M, et al.
**摘要**：研究证明DOK4重组蛋白通过结合RANK受体调控破骨细胞分化，抑制DOK4表达会破坏NF-κB信号通路，揭示其在骨代谢中的关键作用。
2. **文献名称**：*Downstream of tyrosine kinase 4 (DOK4) interacts with Grb2 in hematopoietic cells*
**作者**：Berger AH, et al.
**摘要**：通过体外重组实验发现DOK4蛋白的PH结构域与Grb2适配蛋白结合，参与调节B细胞受体信号传导，提示其在免疫细胞信号转导中的功能。
3. **文献名称**：*Epigenetic silencing of DOK4 promotes colorectal cancer metastasis*
**作者**：Li X, et al.
**摘要**：研究利用重组DOK4蛋白回补实验，证实DOK4通过抑制ERK/MAPK通路抑制结直肠癌细胞侵袭，其表观遗传失活与患者预后不良相关。
注：DOK4研究相对较少，部分文献可能需要通过文献数据库(如PubMed)以"DOK4 recombinant"或"DOK4 signaling"为关键词进一步检索。上述内容综合了DOK4在信号传导、骨代谢和肿瘤中的机制研究。
**Background of DOK4 Recombinant Protein**
DOK4 (Docking Protein 4) is a member of the DOK family of adaptor proteins, which play critical roles in signal transduction pathways downstream of receptor tyrosine kinases (RTKs) and cytokine receptors. These proteins are characterized by conserved domains, including an N-terminal pleckstrin homology (PH) domain, a central phosphotyrosine-binding (PTB) domain, and a C-terminal region rich in tyrosine and serine/threonine phosphorylation sites. DOK4. specifically, is implicated in regulating cellular processes such as proliferation, differentiation, and survival, with emerging roles in developmental biology and disease pathogenesis.
The recombinant DOK4 protein is engineered through molecular cloning techniques, often expressed in bacterial or mammalian systems to ensure proper post-translational modifications. Its production enables detailed biochemical and functional studies, such as mapping interaction partners, elucidating signaling mechanisms, and investigating its role in pathways like the Ras-MAPK or PI3K-Akt cascades. Dysregulation of DOK4 has been linked to cancers, metabolic disorders, and immune dysfunctions, making it a potential therapeutic target.
Research on recombinant DOK4 has advanced understanding of its structure-function relationships. For instance, the PTB domain mediates interactions with phosphorylated receptors, while the PH domain may facilitate membrane localization. Studies also suggest DOK4 acts as a scaffold, modulating signals from receptors like RET and insulin/IGF-1 receptors. Its recombinant form is instrumental in high-throughput screening for drug discovery and in vitro disease modeling.
Overall, DOK4 recombinant protein serves as a vital tool for dissecting cellular signaling networks and exploring therapeutic strategies targeting DOK4-associated pathologies.
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