| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PANX3 |
| Uniprot No | Q96QZ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-392aa |
| 氨基酸序列 | MSLAHTAAEYMLSDALLPDRRGPRLKGLRLELPLDRIVKFVAVGSPLLLMSLAFAQEFSSGSPISCFSPSNFSIRQAAYVDSSCWDSLLHHKQDGPGQDKMKSLWPHKALPYSLLALALLMYLPVLLWQYAAVPALSSDLLFIISELDKSYNRSIRLVQHMLKIRQKSSDPYVFWNELEKARKERYFEFPLLERYLACKQRSHSLVATYLLRNSLLLIFTSATYLYLGHFHLDVFFQEEFSCSIKTGLLSDETHVPNLITCRLTSLSIFQIVSLSSVAIYTILVPVIIYNLTRLCRWDKRLLSVYEMLPAFDLLSRKMLGCPINDLNVILLFLRANISELISFSWLSVLCVLKDTTTQKHNIDTVVDFMTLLAGLEPSKPKHLTNSACDEHP |
| 预测分子量 | 44,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PANX3重组蛋白的参考文献及摘要概述：
1. **文献名称**：*"Recombinant PANX3 regulates chondrogenic differentiation via the ERK1/2 pathway in vitro"*
**作者**：Li Y, et al.
**摘要**：研究利用重组PANX3蛋白处理间充质干细胞，发现其通过激活ERK1/2信号通路促进软骨分化，为骨关节炎治疗提供潜在靶点。
2. **文献名称**：*"Expression and purification of functional human PANX3 in HEK293 cells for channel activity analysis"*
**作者**：Bond SR, et al.
**摘要**：报道了在HEK293细胞中高效表达重组人源PANX3的方法，并验证其形成的膜通道具有ATP释放功能，为后续药理学研究奠定基础。
3. **文献名称**：*"PANX3 recombinant protein enhances osteoblast mineralization by modulating intracellular calcium levels"*
**作者**：Cea LA, et al.
**摘要**：实验表明重组PANX3通过调控成骨细胞钙离子内流，促进矿化结节形成，揭示了其在骨发育中的关键作用。
4. **文献名称**：*"Structural insights into PANX3 channel gating using cryo-EM and recombinant protein technology"*
**作者**：Wang J, et al.
**摘要**：结合冷冻电镜和重组蛋白技术，首次解析了PANX3通道的开放/闭合构象，阐明其pH依赖性门控机制。
注：以上文献信息为模拟示例，实际引用时需核实具体论文是否存在及准确性。建议通过PubMed或Web of Science以关键词“PANX3 recombinant”“Pannexin3 expression”检索最新研究。
**Background of PANX3 Recombinant Protein**
Pannexin 3 (PANX3) is a member of the pannexin family of channel-forming proteins, which are involved in intercellular communication and regulate processes such as ATP release, calcium signaling, and inflammation. PANX3 is primarily expressed in tissues like skin, bone, cartilage, and skeletal muscle, where it plays critical roles in cellular differentiation, proliferation, and apoptosis. Unlike other pannexins (PANX1 and PANX2), PANX3 is uniquely associated with tissue development and homeostasis, particularly in skeletal systems. Studies suggest it modulates osteoblast and chondrocyte differentiation by regulating intracellular signaling pathways, including Wnt/β-catenin and ERK, impacting bone formation and remodeling.
The recombinant PANX3 protein is engineered using heterologous expression systems (e.g., mammalian or bacterial cells) to produce a purified, functional form for research. This allows scientists to study its structure, channel activity, and interactions in controlled settings. Recombinant PANX3 has been instrumental in elucidating its role in diseases such as osteoarthritis, osteoporosis, and cancer, where dysregulated pannexin expression correlates with pathological progression. Additionally, it serves as a tool to screen therapeutic agents targeting pannexin channels, which are emerging as potential drug targets due to their involvement in inflammation and cell death pathways.
Research on PANX3 recombinant protein continues to expand, driven by its dual role as a membrane channel and a regulator of key developmental pathways, offering insights into both basic biology and translational applications.
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