| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MTNR1C |
| Uniprot No | P49219 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-420aa |
| 氨基酸序列 | MMEVNSTCLDCRTPGTIRTEQDAQDSASQGLTSALAVVLIFTIVVDVLGNILVILSVLRNKKLQNAGNLFVVSLSIADLVVAVYPYPVILIAIFQNGWTLGNIHCQISGFLMGLSVIGSVFNITAIAINRYCYICHSLRYDKLYNQRSTWCYLGLTWILTIIAIVPNFFVGSLQYDPRIFSCTFAQTVSSSYTITVVVVHFIVPLSVVTFCYLRIWVLVIQVKHRVRQDFKQKLTQTDLRNFLTMFVVFVLFAVCWAPLNFIGLAVAINPFHVAPKIPEWLFVLSYFMAYFNSCLNAVIYGVLNQNFRKEYKRILMSLLTPRLLFLDTSRGGTEGLKSKPSPAVTNNNQADMLGEARSLWLSRRNGAKMVIIIRPRKAQIAIIHQIFWPQSSWATCRQDTKITGEEDGCRELCKDGISQR |
| 预测分子量 | 47,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MTNR1C重组蛋白的3篇参考文献示例(基于领域内典型研究方向的概括性描述，具体文献需通过学术数据库进一步核实)：
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1. **文献名称**：*"Melatonin receptor MTNR1C regulates human cancer cell proliferation and metastasis through recombinant expression studies"*
**作者**：Liu Y, et al.
**摘要**：该研究通过在大肠杆菌和哺乳动物细胞系统中重组表达MTNR1C蛋白，发现其过表达可抑制多种癌细胞系的迁移和侵袭能力，提示其在肿瘤调控中的潜在作用。
2. **文献名称**：*"Pharmacological characterization of recombinant human MTNR1C receptor signaling pathways"*
**作者**：Jockers R, et al.
**摘要**：作者利用杆状病毒-昆虫细胞系统表达并纯化MTNR1C重组蛋白，通过cAMP和β-arrestin检测实验，揭示了其与褪黑素结合后激活G蛋白依赖和非依赖信号通路的分子机制。
3. **文献名称**：*"Optimization of MTNR1C recombinant protein production in E. coli for structural studies"*
**作者**：Baba K, et al.
**摘要**：本研究优化了MTNR1C的跨膜结构域在大肠杆菌中的可溶性表达条件，并通过亲和层析和圆二色谱验证其正确折叠，为后续结构解析奠定基础。
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**注意**：以上文献信息为示例性质，实际研究中MTNR1C的相关文献可能较少(因MTNR1A/1B研究更为常见)。建议通过PubMed或Web of Science以“MTNR1C recombinant protein”或“MTNR1C expression”为关键词检索最新文献。若需具体文献，可提供更详细的研究方向(如表达系统或功能分析)。
The MTNR1C protein, a member of the melatonin receptor family, belongs to the G protein-coupled receptor (GPCR) superfamily. While MTNR1A (MT1) and MTNR1B (MT2) are well-characterized receptors mediating melatonin's circadian and neuroendocrine effects, MTNR1C remains less studied. Initially identified as a pseudogene in humans due to sequence variations and lack of functional evidence, recent studies suggest its potential expression in specific tissues, though its physiological relevance is debated.
Recombinant MTNR1C protein is engineered for functional studies, typically expressed in heterologous systems like HEK293 or insect cells. Its structural features include seven transmembrane domains and conserved motifs shared with MT1/MT2. but ligand-binding assays show weak or absent melatonin affinity. This raises questions about its role as a melatonin receptor or its possible involvement in non-canonical signaling pathways.
Research focuses on resolving its biological significance: Some evidence links MTNR1C to metabolic regulation and cancer progression via unclear mechanisms. Recombinant versions enable exploration of dimerization with MT1/MT2 receptors, potentially modulating their activity. Challenges persist in confirming endogenous expression and distinguishing it from cross-reactivity with other receptors.
Pharmaceutical interest lies in its atypical GPCR behavior, offering insights into receptor evolution and allosteric modulation. While not a direct drug target, MTNR1C recombinant protein serves as a tool to decode melatonin receptor complexity and expand understanding of GPCR diversity. Further studies are needed to clarify its cellular localization, signaling partners, and species-specific functional divergence.
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