| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AKAP5 |
| Uniprot No | P24588 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-427aa |
| 氨基酸序列 | METTISEIHV ENKDEKRSAE GSPGAERQKE KASMLCFKRR KKAAKALKPK AGSEAADVAR KCPQEAGASD QPEPTRGAWA SLKRLVTRRK RSESSKQQKP LEGEMQPAIN AEDADLSKKK AKSRLKIPCI KFPRGPKRSN HSKIIEDSDC SIKVQEEAEI LDIQTQTPLN DQATKAKSTQ DLSEGISRKD GDEVCESNVS NSTTSGEKVI SVELGLDNGH SAIQTGTLIL EEIETIKEKQ DVQPQQASPL ETSETDHQQP VLSDVPPLPA IPDQQIVEEA SNSTLESAPN GKDYESTEIV AEETKPKDTE LSQESDFKEN GITEEKSKSE ESKRMEPIAI IITDTEISEF DVTKSKNVPK QFLISAENEQ VGVFANDNGF EDRTSEQYET LLIETASSLV KNAIQLSIEQ LVNEMASDDN KINNLLQ |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AKAP5重组蛋白的假设性参考文献示例，概括了相关研究方向：
1. **文献名称**：*"High-yield expression and purification of recombinant AKAP5 for functional studies of cAMP-PKA signaling"*
**作者**：Chen, L., et al.
**摘要**：研究报道了在大肠杆菌系统中优化AKAP5重组蛋白的表达与纯化流程，并验证其与PKA调控亚基(RI/RII)的结合能力，证实其在体外模型中增强cAMP信号的空间特异性。
2. **文献名称**：*"AKAP5 recombinant protein restores β-adrenergic receptor trafficking in AKAP5-deficient cardiomyocytes"*
**作者**：Wang, Y., et al.
**摘要**：通过将重组AKAP5蛋白导入缺乏内源性AKAP5的心肌细胞，证明其能够挽救β-肾上腺素受体-PKA信号通路缺陷，改善钙离子调控及心脏收缩功能。
3. **文献名称**：*"Cryo-EM structure of the AKAP5-PKA-C complex reveals dynamic anchoring mechanisms"*
**作者**：Rodriguez, S., et al.
**摘要**：利用重组AKAP5蛋白与PKA催化亚基(PKA-C)进行复合物组装，通过冷冻电镜解析其三维结构，揭示了AKAP5调控PKA底物选择性的分子基础。
4. **文献名称**：*"Targeted disruption of AKAP5-PKA interaction using recombinant competitive peptides in neuronal signaling"*
**作者**：Kim, H., et al.
**摘要**：设计并表达基于AKAP5功能域的重组竞争性多肽，证明其可阻断AKAP5-PKA相互作用，抑制海马神经元中与记忆相关的突触信号传导。
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**注**：上述文献为假设性示例，实际研究中请通过学术数据库(如PubMed、Google Scholar)检索具体文献。建议使用关键词“AKAP5 recombinant protein expression”、“AKAP5-PKA interaction”或“AKAP5 signaling”获取最新成果。
A-kinase anchoring protein 5 (AKAP5), also known as AKAP75/79. is a scaffold protein that organizes cyclic AMP (cAMP)-dependent signaling complexes by tethering protein kinase A (PKA), phosphodiesterases (PDEs), and other effectors to specific subcellular locations. It plays a critical role in compartmentalizing cAMP/PKA signaling near plasma membranes, particularly in neurons, cardiac myocytes, and immune cells. AKAP5 contains conserved domains for PKA binding via its N-terminal region, membrane association through palmitoylation sites, and interactions with receptors, ion channels, or phosphatases. This spatial regulation ensures precise control of downstream processes like ion channel modulation, neurotransmitter release, and calcium handling.
Recombinant AKAP5 proteins are engineered to study its structure-function relationships and signaling networks. Produced in bacterial or mammalian systems, these proteins often include affinity tags (e.g., His, GST) for purification and detection. Researchers use them to map binding interfaces, dissect cAMP/PKA dynamics, and identify disruptions in AKAP5-mediated signaling linked to arrhythmias, neurological disorders, or immune dysregulation. Additionally, AKAP5 recombinant tools aid in drug discovery, enabling high-throughput screening for molecules targeting pathological AKAP5 interactions while preserving physiological functions. Its role in diseases like heart failure and cancer underscores its therapeutic relevance. By reconstituting AKAP5 complexes in vitro, these proteins help unravel how localized cAMP signaling orchestrates cellular responses and how its dysregulation contributes to disease.
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