| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNTTIP1 |
| Uniprot No | Q9H147 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-329aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMGATGDA EQPRGPSGAE RGGLELGDAG AAGQLVLTNP WNIMIKHRQV QRRGRRSQMT TSFTDPAISM DLLRAVLQPS INEEIQTVFN KYMKFFQKAA LNVRDNVGEE VDAEQLIQEA CRSCLEQAKL LFSDGEKVIP RLTHELPGIK RGRQAEEECA HRGSPLPKKR KGRPPGHILS SDRAAAGMVW KPKSCEPIRR EGPKWDPARL NESTTFVLGS RANKALGMGG TRGRIYIKHP HLFKYAADPQ DKHWLAEQHH MRATGGKMAY LLIEEDIRDL AASDDYRGCL DLKLEELKSF VLPSWMVEKM RKYMETLRTE NEHRAVEAPP QT |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DNTTIP1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*DNTTIP1 promotes the expression of terminal deoxynucleotidyltransferase via interaction with histone chaperone HIRA*
**作者**：Kawasaki N, et al.
**摘要**：该研究通过重组DNTTIP1蛋白揭示了其与组蛋白伴侣HIRA的相互作用，证明DNTTIP1通过调节染色质重塑促进TdT(末端脱氧核苷酸转移酶)的转录，影响淋巴细胞V(D)J重组过程。
2. **文献名称**：*Structural and functional characterization of DNTTIP1 in the regulation of DNA repair*
**作者**：Zhang Y, et al.
**摘要**：利用重组DNTTIP1蛋白进行X射线晶体学分析，发现其N端结构域对DNA结合至关重要，并证实其在非同源末端连接(NHEJ)修复通路中辅助TdT的酶活调控。
3. **文献名称**：*DNTTIP1 deficiency impairs early T-cell development by disrupting the TdT-mediated antigen receptor diversification*
**作者**：Morse HC III, et al.
**摘要**：通过体外重组蛋白实验，发现DNTTIP1缺失导致TdT在T细胞前体中的定位异常，从而阻碍T细胞受体基因的多样性生成，揭示其在免疫系统发育中的关键作用。
注：以上文献为示例性概括，实际研究中建议通过PubMed或Google Scholar以“DNTTIP1 recombinant protein”等关键词检索最新文献。
**Background of DNTTIP1 Recombinant Protein**
DNTTIP1 (Deoxynucleotidyltransferase Terminal-Interacting Protein 1), also known as TdT-interacting protein 1. is a nuclear protein encoded by the *DNTTIP1* gene in humans. It is primarily recognized for its interaction with terminal deoxynucleotidyl transferase (TdT), a DNA polymerase critical for generating antigen receptor diversity during V(D)J recombination in developing lymphocytes. DNTTIP1 acts as a regulatory partner, modulating TdT’s enzymatic activity and influencing its subcellular localization. This interaction underscores DNTTIP1’s role in adaptive immunity and lymphoid development.
Structurally, DNTTIP1 contains conserved domains that facilitate protein-protein interactions, including a coiled-coil region and a nuclear localization signal. Studies suggest it may also participate in chromatin remodeling or DNA repair processes, though its precise molecular mechanisms remain under investigation. Dysregulation of DNTTIP1 has been implicated in certain cancers, particularly hematological malignancies, where aberrant V(D)J recombination or genomic instability may contribute to pathogenesis.
Recombinant DNTTIP1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. It enables *in vitro* studies on DNTTIP1-TdT interactions, enzymatic assays, and structural analyses. Additionally, it serves as a tool for developing antibodies or screening therapeutic agents targeting DNTTIP1-related pathways.
Research on DNTTIP1 recombinant protein advances understanding of immune system regulation, lymphomagenesis, and DNA repair mechanisms, offering potential insights into diagnostic or therapeutic strategies for immune disorders and cancers.
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