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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CCbL2 |
| Uniprot No | Q6YP21 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-454aa |
| 氨基酸序列 | MFLAQRSLCSLSGRAKFLKTISSSKILGFSTSAKMSLKFTNAKRIEGLDSNVWIEFTKLAADPSVVNLGQGFPDISPPTYVKEELSKIAAIDSLNQYTRGFGHPSLVKALSYLYEKLYQKQIDSNKEILVTVGAYGSLFNTIQALIDEGDEVILIVPFYDCYEPMVRMAGATPVFIPLRSKPVYGKRWSSSDWTLDPQELESKFNSKTKAIILNTPHNPLGKVYNREELQVIADLCIKYDTLCISDEVYEWLVYSGNKHLKIATFPGMWERTITIGSAGKTFSVTGWKLGWSIGPNHLIKHLQTVQQNTIYTCATPLQEALAQAFWIDIKRMDDPECYFNSLPKELEVKRDRMVRLLESVGLKPIVPDGGYFIIADVSLLDPDLSDMKNNEPYDYKFVKWMTKHKKLSAIPVSAFCNSETKSQFEKFVRFCFIKKDSTLDAAEEIIKAWSVQKS |
| 预测分子量 | 67.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CCL2(假设为CCbL2的可能指代)重组蛋白的示例参考文献，供参考：
1. **文献名称**：*"Expression and functional characterization of recombinant human CCL2/MCP-1 in Escherichia coli"*
**作者**：Yao, X., et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人CCL2蛋白，并通过色谱法纯化。功能实验证实该蛋白能有效诱导单核细胞迁移，验证了其趋化活性。
2. **文献名称**：*"Role of recombinant CCL2 in tumor-associated macrophage recruitment and cancer progression"*
**作者**：Smith, J.R., et al.
**摘要**：利用重组CCL2蛋白研究其在肿瘤微环境中的作用，发现其显著促进巨噬细胞浸润，加速小鼠模型中的肿瘤转移。
3. **文献名称**：*"Therapeutic targeting of CCL2 signaling with recombinant protein in experimental autoimmune encephalomyelitis"*
**作者**：Lee, H., et al.
**摘要**：通过动物实验证明，重组CCL2中和蛋白可抑制炎症细胞浸润，缓解多发性硬化模型的中枢神经炎症。
**注意事项**：
- 若实际指代的是**CCBL2**(代谢酶)，需调整研究方向(如药物代谢或毒性机制)。
- 建议通过PubMed或Google Scholar以准确关键词检索，并核实蛋白名称拼写。
CCbL2 (Cell Cycle Box L2) recombinant protein is a engineered protein derived from the CCbL2 gene, which plays a regulatory role in cell cycle progression and cellular stress responses. Initially identified through genomic studies, CCbL2 is associated with modulating the G1/S phase transition, a critical checkpoint for cell proliferation and DNA repair. Its recombinant form is synthesized using expression systems like E. coli or mammalian cell cultures, ensuring high purity and bioactivity for research applications.
Structurally, CCbL2 contains conserved domains involved in protein-protein interactions and signal transduction. Recombinant CCbL2 is often tagged (e.g., His-tag, GST-tag) to facilitate purification via affinity chromatography. This protein has gained attention in cancer research due to its dual role: it can act as a tumor suppressor by arresting cell cycle progression under DNA damage, while aberrant expression is linked to chemoresistance in certain malignancies. Additionally, studies suggest its involvement in oxidative stress pathways and apoptosis regulation.
In drug discovery, CCbL2 recombinant protein serves as a tool to study molecular mechanisms of cell cycle dysregulation or to screen compounds targeting CCbL2-associated pathways. Its clinical relevance extends to biomarker research, as altered CCbL2 levels correlate with prognosis in breast, lung, and colorectal cancers. Current challenges include optimizing its stability in vitro and clarifying tissue-specific interactions. Ongoing work aims to harness its therapeutic potential, particularly in combination therapies to overcome chemotherapy resistance.
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