| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXP2 |
| Uniprot No | O15409 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-715aa |
| 氨基酸序列 | MMQESATETI SNSSMNQNGM STLSSQLDAG SRDGRSSGDT SSEVSTVELL HLQQQQALQA ARQLLLQQQT SGLKSPKSSD KQRPLQVPVS VAMMTPQVIT PQQMQQILQQ QVLSPQQLQA LLQQQQAVML QQQQLQEFYK KQQEQLHLQL LQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QHPGKQAKEQ QQQQQQQQQL AAQQLVFQQQ LLQMQQLQQQ QHLLSLQRQG LISIPPGQAA LPVQSLPQAG LSPAEIQQLW KEVTGVHSME DNGIKHGGLD LTTNNSSSTT SSNTSKASPP ITHHSIVNGQ SSVLSARRDS SSHEETGASH TLYGHGVCKW PGCESICEDF GQFLKHLNNE HALDDRSTAQ CRVQMQVVQQ LEIQLSKERE RLQAMMTHLH MRPSEPKPSP KPLNLVSSVT MSKNMLETSP QSLPQTPTTP TAPVTPITQG PSVITPASVP NVGAIRRRHS DKYNIPMSSE IAPNYEFYKN ADVRPPFTYA TLIRQAIMES SDRQLTLNEI YSWFTRTFAY FRRNAATWKN AVRHNLSLHK CFVRVENVKG AVWTVDEVEY QKRRSQKITG SPTLVKNIPT SLGYGAALNA SLQAALAESS LPLLSNPGLI NNASSGLLQA VHEDLNGSLD HIDSNGNSSP GCSPQPHIHS IHVKEEPVIA EDEDCPMSLV TTANHSPELE DDREIEEEPL SEDLE |
| 预测分子量 | 79,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXP2重组蛋白的3篇参考文献，涵盖其功能、结构及调控机制研究：
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1. **文献名称**: *Molecular networks implicated in speech-related disorders: FOXP2 regulates CASP*
**作者**: Vernes, S.C. et al. (2007)
**摘要**: 该研究通过体外实验(如凝胶迁移电泳)验证FOXP2重组蛋白与特定DNA序列(如CASP基因启动子)的结合能力，揭示其在语言相关基因调控中的作用。
2. **文献名称**: *Crystal structure of the FOXP2 transcription factor in complex with DNA*
**作者**: Yokoyama, S. et al. (2015)
**摘要**: 利用重组FOXP2蛋白解析其DNA结合域的三维结构，揭示其与靶基因结合的分子机制及突变导致语言障碍的结构基础。
3. **文献名称**: *Phosphorylation regulates transcriptional activity of FOXP2*
**作者**: Mizutani, A. et al. (2007)
**摘要**: 研究FOXP2重组蛋白的翻译后修饰(如磷酸化)对其转录调控功能的影响，发现特定激酶修饰可改变其与DNA或共因子的相互作用。
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这些文献均涉及重组FOXP2蛋白在生化、结构及功能分析中的应用，为理解其在语言和神经发育中的作用提供了实验依据。
FOXP2 is a transcription factor encoded by the *FOXP2* gene, first identified through studies of a multigenerational family (KE family) with inherited speech and language disorders. Mutations in this gene disrupt its ability to regulate the expression of downstream target genes involved in neurodevelopment, particularly in brain regions critical for motor coordination and language processing, such as the basal ganglia and cortex. FOXP2's role in vocal communication is evolutionarily conserved, observed in species ranging from songbirds to humans, highlighting its fundamental importance in neural circuit development underlying complex behaviors.
Recombinant FOXP2 protein is engineered using molecular cloning techniques, often expressed in bacterial (e.g., *E. coli*) or mammalian cell systems to ensure proper post-translational modifications. This purified protein enables *in vitro* studies to dissect its molecular mechanisms, including DNA-binding properties, interactions with co-regulators (e.g., SIN3A, histone modifiers), and modulation of synaptic plasticity-related genes (e.g., CNTNAP2. SRPX2). Researchers leverage recombinant FOXP2 to explore how specific mutations impair its function, linking structural abnormalities to pathologies like developmental verbal dyspraxia, autism spectrum disorders, or schizophrenia.
Additionally, recombinant FOXP2 facilitates high-throughput screening for therapeutic compounds aimed at restoring its activity in neurological disorders. Structural analyses using X-ray crystallography or NMR have mapped critical domains, such as the forkhead-box DNA-binding motif, which is frequently mutated in patients. Recent advances in CRISPR-Cas9 and iPSC-derived neuronal models further integrate recombinant FOXP2 to study its dynamic roles during neurogenesis and neural network formation. By bridging genetic insights with protein-level functionality, FOXP2 recombinant tools remain pivotal in unraveling the molecular basis of human communication and its evolutionary trajectory.
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