| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXF1 |
| Uniprot No | Q12946 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-379aa |
| 氨基酸序列 | MSSAPEKQQP PHGGGGGGGG GGGAAMDPAS SGPSKAKKTN AGIRRPEKPP YSYIALIVMA IQSSPTKRLT LSEIYQFLQS RFPFFRGSYQ GWKNSVRHNL SLNECFIKLP KGLGRPGKGH YWTIDPASEF MFEEGSFRRR PRGFRRKCQA LKPMYSMMNG LGFNHLPDTY GFQGSAGGLS CPPNSLALEG GLGMMNGHLP GNVDGMALPS HSVPHLPSNG GHSYMGGCGG AAAGEYPHHD SSVPASPLLP TGAGGVMEPH AVYSGSAAAW PPSASAALNS GASYIKQQPL SPCNPAANPL SGSLSTHSLE QPYLHQNSHN APAELQGIPR YHSQSPSMCD RKEFVFSFNA MASSSMHSAG GGSYYHQQVT YQDIKPCVM |
| 预测分子量 | 40,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于FOXF1重组蛋白的相关文献摘要，按研究重点分类整理：
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1. **文献名称**：*FOXF1 Recombinant Protein Enhances Mesenchymal Stem Cell Migration via MAPK/ERK Signaling*
**作者**：Li Y, et al.
**摘要**：研究通过大肠杆菌表达系统纯化FOXF1重组蛋白，发现其可激活间充质干细胞中MAPK/ERK通路，显著促进细胞迁移能力，提示其在组织修复中的潜在应用。
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2. **文献名称**：*Structural Analysis of FOXF1 DNA-Binding Domain Using Recombinant Protein Crystallography*
**作者**：Smith J, et al.
**摘要**：利用昆虫细胞表达系统制备FOXF1重组蛋白的DNA结合结构域，通过X射线晶体学解析其三维结构，揭示了其与靶基因启动子区域的特异性结合机制。
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3. **文献名称**：*FOXF1 Recombinant Protein Attenuates Pulmonary Fibrosis in Murine Models*
**作者**：Wang H, et al.
**摘要**：在小鼠肺纤维化模型中，鼻内递送FOXF1重组蛋白显著抑制TGF-β信号通路，减少胶原沉积，表明其作为抗纤维化治疗剂的潜力。
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**注**：上述文献信息为示例性内容，实际引用时需根据具体文献调整。建议通过PubMed或Web of Science以“FOXF1 recombinant protein”为关键词检索最新实证研究。
FOXF1 recombinant protein is a biologically engineered molecule derived from the Forkhead box (FOX) family of transcription factors, specifically the FOXF1 gene. FOXF1 plays a critical role in embryonic development, particularly in the formation of mesoderm-derived tissues such as the lungs, gastrointestinal tract, and vascular system. It regulates gene expression by binding to DNA through its conserved forkhead domain, influencing cell differentiation, proliferation, and tissue homeostasis. Dysregulation of FOXF1 is associated with congenital disorders (e.g., alveolar capillary dysplasia) and pathological processes like fibrosis and cancer metastasis, highlighting its therapeutic and diagnostic relevance.
Recombinant FOXF1 protein is typically produced using expression systems such as *E. coli* or mammalian cells, enabling large-scale purification for research or clinical applications. The protein often includes tags (e.g., His-tag) for ease of isolation and may retain post-translational modifications when expressed in eukaryotic systems. Structurally, it contains the DNA-binding domain critical for its transcriptional activity, allowing studies on its interaction with target genes or signaling pathways like TGF-β and BMP.
Researchers utilize FOXF1 recombinant protein to investigate its role in developmental biology, tissue repair, and disease mechanisms. It serves as a tool for *in vitro* assays, cell culture studies, and animal models to explore its potential in regenerative medicine or anticancer therapies. Additionally, it aids in drug screening to identify modulators of FOXF1 activity. Its application extends to understanding epigenetic regulation, as FOXF1 expression is often silenced in cancers through promoter hypermethylation. Overall, FOXF1 recombinant protein bridges molecular biology and translational research, offering insights into both developmental pathways and therapeutic strategies.
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