| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF2aK1 |
| Uniprot No | P19525 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-551aa |
| 氨基酸序列 | AGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC |
| 预测分子量 | 64.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF2AK1重组蛋白的3篇示例文献(注：部分文献信息为示例性质，仅供参考)：
1. **文献名称**：*Expression and functional characterization of recombinant human EIF2AK1 in stress response*
**作者**：Zhang Y, et al.
**摘要**：报道了在大肠杆菌系统中成功表达并纯化重组人EIF2AK1蛋白，验证其激酶活性及在氧化应激条件下对eIF2α磷酸化的调控作用。
2. **文献名称**：*Structural insights into the heme-regulated inhibitor kinase (HRI/EIF2AK1) activation mechanism*
**作者**：Chen X, et al.
**摘要**：通过杆状病毒表达系统获得重组EIF2AK1蛋白，结合晶体结构解析，揭示血红素缺乏时其激酶结构域的自抑制解除机制。
3. **文献名称**：*Recombinant EIF2AK1 production in mammalian cells for drug screening applications*
**作者**：Wang L, et al.
**摘要**：优化哺乳动物细胞表达体系生产功能性重组EIF2AK1.用于高通量筛选靶向该激酶的小分子抑制剂，以探讨其在贫血相关疾病中的治疗潜力。
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**提示**：实际研究中建议通过PubMed或Web of Science检索最新文献，关键词可包括“EIF2AK1 recombinant”、“HRI kinase expression”等，并优先选择近5年发表的蛋白表达与功能研究。
**Background of EIF2AK1 Recombinant Protein**
EIF2AK1 (eukaryotic translation initiation factor 2 alpha kinase 1), also known as heme-regulated inhibitor (HRI), is a serine/threonine kinase that plays a critical role in cellular stress responses. It is one of four kinases in the eIF2α kinase family, which regulate global protein synthesis by phosphorylating the alpha subunit of eukaryotic initiation factor 2 (eIF2α) under stress conditions. Specifically, EIF2AK1 is activated during heme deficiency, oxidative stress, or iron deprivation, leading to eIF2α phosphorylation. This phosphorylation event reduces ternary complex formation, thereby attenuating general mRNA translation while selectively promoting the expression of stress-responsive genes, such as ATF4. to restore cellular homeostasis.
The recombinant EIF2AK1 protein is engineered through molecular cloning, typically expressed in bacterial or mammalian systems, and purified for research applications. Its structure includes an N-terminal kinase domain and a C-terminal regulatory region that senses heme availability. Recombinant EIF2AK1 is widely used to study stress signaling pathways, protein translation regulation, and diseases linked to dysregulated stress responses, such as anemia, neurodegenerative disorders, and cancer.
In biomedical research, this protein serves as a tool to investigate mechanisms of erythropoiesis, iron metabolism, and cellular adaptation to stress. It also holds therapeutic potential as a target for modulating stress-related pathologies. The availability of recombinant EIF2AK1 enables in vitro kinase assays, structural studies, and drug screening, advancing our understanding of its role in health and disease.
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