| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PANX2 |
| Uniprot No | Q96RD6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-677aa |
| 氨基酸序列 | MHHLLEQSADMATALLAGEKLRELILPGAQDDKAGALAALLLQLKLELPFDRVVTIGTVLVPILLVTLVFTKNFAEEPIYCYTPHNFTRDQALYARGYCWTELRDALPGVDASLWPSLFEHKFLPYALLAFAAIMYVPALGWEFLASTRLTSELNFLLQEIDNCYHRAAEGRAPKIEKQIQSKGPGITEREKREIIENAEKEKSPEQNLFEKYLERRGRSNFLAKLYLARHVLILLLSAVPISYLCTYYATQKQNEFTCALGASPDGAAGAGPAVRVSCKLPSVQLQRIIAGVDIVLLCVMNLIILVNLIHLFIFRKSNFIFDKLHKVGIKTRRQWRRSQFCDINILAMFCNENRDHIKSLNRLDFITNESDLMYDNVVRQLLAALAQSNHDATPTVRDSGVQTVDPSANPAEPDGAAEPPVVKRPRKKMKWIPTSNPLPQPFKEPLAIMRVENSKAEKPKPARRKTATDTLIAPLLDRSAHHYKGGGGDPGPGPAPAPAPPPAPDKKHARHFSLDVHPYILGTKKAKAEAVPAALPASRSQEGGFLSQAEDCGLGLAPAPIKDAPLPEKEIPYPTEPARAGLPSGGPFHVRSPPAAPAVAPLTPASLGKAEPLTILSRNATHPLLHINTLYEAREEEDGGPRLPQDVGDLIAIPAPQQILIATFDEPRTVVSTVEF |
| 预测分子量 | 74,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PANX2重组蛋白的虚构参考文献示例(基于领域研究趋势，非真实文献)：
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1. **文献名称**: "Recombinant PANX2 Expression in Mammalian Cells: Purification and Channel Activity Analysis"
**作者**: Zhang L, et al.
**摘要**: 本研究报道了在HEK293细胞中重组表达人源PANX2蛋白的优化方法，通过亲和层析纯化获得高纯度蛋白。电生理实验表明PANX2形成ATP选择性通道，其活性受胞内钙离子浓度调控，提示其在神经胶质细胞间通讯中的潜在作用。
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2. **文献名称**: "Structural Insights into PANX2 Hemichannels by Cryo-EM"
**作者**: Watanabe K, et al.
**摘要**: 利用冷冻电镜解析了重组PANX2蛋白的3.8 Å分辨率结构，揭示了其七聚体组装模式及跨膜区关键氨基酸分布。结构比对发现PANX2与PANX1的孔道直径差异可能解释二者底物选择性的不同。
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3. **文献名称**: "PANX2 Knockdown Enhances Apoptosis in Glioblastoma Cells via ER Stress Pathway"
**作者**: Chen R, et al.
**摘要**: 通过重组PANX2 siRNA在胶质母细胞瘤模型中的递送实验，发现PANX2沉默可激活内质网应激通路并促进肿瘤细胞凋亡，提示PANX2可能作为胶质瘤治疗的潜在靶点。
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(注：以上文献为模拟示例，实际研究中请通过PubMed/Google Scholar检索真实文献。)
Pannexin 2 (PANX2), a member of the pannexin family of large-pore channel-forming proteins, plays a role in cellular communication by facilitating the regulated exchange of ions, metabolites, and signaling molecules across membranes. The pannexin family comprises three isoforms (PANX1. PANX2. PANX3), with PANX2 being the least characterized compared to the well-studied PANX1. Structurally, PANX2 shares a conserved four-transmembrane domain architecture but exhibits distinct tissue distribution and functional properties. It is predominantly expressed in the central nervous system, particularly in neurons and glial cells, suggesting involvement in neurophysiological processes such as synaptic plasticity, inflammation, and cellular stress responses.
Recombinant PANX2 protein is engineered for in vitro studies to elucidate its channel properties, post-translational modifications, and interactions with signaling partners. Unlike PANX1. which forms ATP-releasing channels linked to purinergic signaling, PANX2 may function in intracellular compartments (e.g., endoplasmic reticulum) and influence autophagy or apoptosis. However, its exact physiological role remains debated, partly due to overlapping functions with other pannexins and technical challenges in distinguishing endogenous PANX2 activity.
Research using recombinant PANX2 has revealed its sensitivity to redox conditions and caspase cleavage, implicating it in neurodegenerative diseases and cancer. For instance, PANX2 dysregulation is observed in glioblastoma and Alzheimer’s models, though mechanistic insights are limited. The recombinant protein enables structural studies (e.g., cryo-EM) and drug screening to identify modulators targeting pannexin-related pathologies. Despite progress, unresolved questions persist regarding PANX2’s activation mechanisms, substrate selectivity, and tissue-specific roles, underscoring the need for further exploration using recombinant tools and conditional knockout models.
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