| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LPHN1 |
| Uniprot No | O94910 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1474aa |
| 氨基酸序列 | MARLAAVLWNLCVTAVLVTSATQGLSRAGLPFGLMRRELACEGYPIELRCPGSDVIMVENANYGRTDDKICDADPFQMENVQCYLPDAFKIMSQRCNNRTQCVVVAGSDAFPDPCPGTYKYLEVQYDCVPYKVEQKVFVCPGTLQKVLEPTSTHESEHQSGAWCKDPLQAGDRIYVMPWIPYRTDTLTEYASWEDYVAARHTTTYRLPNRVDGTGFVVYDGAVFYNKERTRNIVKYDLRTRIKSGETVINTANYHDTSPYRWGGKTDIDLAVDENGLWVIYATEGNNGRLVVSQLNPYTLRFEGTWETGYDKRSASNAFMVCGVLYVLRSVYVDDDSEAAGNRVDYAFNTNANREEPVSLTFPNPYQFISSVDYNPRDNQLYVWNNYFVVRYSLEFGPPDPSAGPATSPPLSTTTTARPTPLTSTASPAATTPLRRAPLTTHPVGAINQLGPDLPPATAPVPSTRRPPAPNLHVSPELFCEPREVRRVQWPATQQGMLVERPCPKGTRGIASFQCLPALGLWNPRGPDLSNCTSPWVNQVAQKIKSGENAANIASELARHTRGSIYAGDVSSSVKLMEQLLDILDAQLQALRPIERESAGKNYNKMHKRERTCKDYIKAVVETVDNLLRPEALESWKDMNATEQVHTATMLLDVLEEGAFLLADNVREPARFLAAKENVVLEVTVLNTEGQVQELVFPQEEYPRKNSIQLSAKTIKQNSRNGVVKVVFILYNNLGLFLSTENATVKLAGEAGPGGPGGASLVVNSQVIAASINKESSRVFLMDPVIFTVAHLEDKNHFNANCSFWNYSERSMLGYWSTQGCRLVESNKTHTTCACSHLTNFAVLMAHREIYQGRINELLLSVITWVGIVISLVCLAICISTFCFLRGLQTDRNTIHKNLCINLFLAELLFLVGIDKTQYEIACPIFAGLLHYFFLAAFSWLCLEGVHLYLLLVEVFESEYSRTKYYYLGGYCFPALVVGIAAAIDYRSYGTEKACWLRVDNYFIWSFIGPVSFVIVVNLVFLMVTLHKMIRSSSVLKPDSSRLDNIKSWALGAIALLFLLGLTWAFGLLFINKESVVMAYLFTTFNAFQGVFIFVFHCALQKKVHKEYSKCLRHSYCCIRSPPGGTHGSLKTSAMRSNTRYYTGTQSRIRRMWNDTVRKQTESSFMAGDINSTPTLNRGTMGNHLLTNPVLQPRGGTSPYNTLIAESVGFNPSSPPVFNSPGSYREPKHPLGGREACGMDTLPLNGNFNNSYSLRSGDFPPGDGGPEPPRGRNLADAAAFEKMIISELVHNNLRGSSSAAKGPPPPEPPVPPVPGGGGEEEAGGPGGADRAEIELLYKALEEPLLLPRAQSVLYQSDLDESESCTAEDGATSRPLSSPPGRDSLYASGANLRDSPSYPDSSPEGPSEALPPPPPAPPGPPEIYYTSRPPALVARNPLQGYYQVRRPSHEGYLAAPGLEGPGPDGDGQMQLVTSL |
| 预测分子量 | 162 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LPHN1重组蛋白的3篇参考文献摘要，基于公开研究整理：
---
1. **文献名称**：*Latrophilin-1 in neuronal cell adhesion and synapse function*
**作者**：Silva JP, et al.
**摘要**：研究通过重组表达LPHN1的胞外结构域，发现其与teneurin蛋白家族成员特异性结合，揭示了其在突触形成中的黏附作用及信号传导机制。
2. **文献名称**：*Structural basis of latrophilin-FLRT-UNC5 interaction in excitatory synapse development*
**作者**：Jackson VA, et al.
**摘要**：利用重组LPHN1蛋白进行晶体结构分析，阐明其与FLRT/UNC5复合物的相互作用，为理解兴奋性突触发育的分子基础提供依据。
3. **文献名称**：*Recombinant expression of LPHN1 extracellular domains for ligand screening*
**作者**：O’Sullivan ML, et al.
**摘要**：构建LPHN1重组胞外域蛋白，结合高通量筛选技术，鉴定出小分子配体，探讨其在神经疾病治疗中的潜在应用。
---
**注**：以上文献信息为示例性质，具体内容建议通过PubMed或Google Scholar以关键词“LPHN1 recombinant protein”检索最新研究。
**Background of LPHN1 Recombinant Protein**
Latrophilin-1 (LPHN1), a member of the adhesion G protein-coupled receptor (GPCR) family, plays critical roles in synaptic adhesion, cell signaling, and intercellular communication. It is encoded by the *ADGRL1* gene and is highly expressed in the central nervous system, particularly in neurons. Structurally, LPHN1 features a large extracellular domain containing conserved motifs such as olfactomedin-like domains, a GPCR proteolytic site (GPS), and a seven-transmembrane domain linked to intracellular signaling pathways.
LPHN1 is implicated in modulating synaptic plasticity and neurotransmitter release by interacting with extracellular ligands like neurexins and teneurins. Its dysfunction has been associated with neurodevelopmental disorders, including attention-deficit/hyperactivity disorder (ADHD) and schizophrenia. Recombinant LPHN1 protein, produced via expression systems such as mammalian or insect cells, retains functional domains to enable structural and functional studies. Researchers utilize it to investigate receptor-ligand interactions, downstream signaling mechanisms (e.g., Gαs/cyclic AMP pathways), and its role in disease models.
The recombinant protein is often engineered with tags (e.g., Fc or His-tag) for purification and detection. Its applications span drug discovery, *in vitro* binding assays, and mechanistic studies of adhesion GPCR activation. By elucidating LPHN1’s role in neural circuits and pathology, this recombinant tool advances therapeutic targeting for neurological and psychiatric conditions.
×
