| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AKAP1 |
| Uniprot No | Q92667 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-903aa |
| 氨基酸序列 | MAIQFRSLFPLALPGMLALLGWWWFFSRKKGHVSSHDEQQVEAGAVQLRADPAIKEPLPVEDVCPKVVSTPPSVTEPPEKELSTVSKLPAEPPALLQTHPPCRRSESSGILPNTTDMRLRPGTRRDDSTKLELALTGGEAKSIPLECPLSSPKGVLFSSKSAEVCKQDSPFSRVPRKVQPGYPVVPAEKRSSGERARETGGAEGTGDAVLGEKVLEEALLSREHVLELENSKGPSLASLEGEEDKGKSSSSQVVGPVQEEEYVAEKLPSRFIESAHTELAKDDAAPAPPVADAKAQDRGVEGELGNEESLDRNEEGLDRNEEGLDRNEESLDRNEEGLDRNEEIKRAAFQIISQVISEATEQVLATTVGKVAGRVCQASQLQGQKEESCVPVHQKTVLGPDTAEPATAEAAVAPPDAGLPLPGLPAEGSPPPKTYVSCLKSLLSSPTKDSKPNISAHHISLASCLALTTPSEELPDRAGILVEDATCVTCMSDSSQSVPLVASPGHCSDSFSTSGLEDSCTETSSSPRDKAITPPLPESTVPFSNGVLKGELSDLGAEDGWTMDAEADHSGGSDRNSMDSVDSCCSLKKTESFQNAQAGSNPKKVDLIIWEIEVPKHLVGRLIGKQGRYVSFLKQTSGAKIYISTLPYTQSVQICHIEGSQHHVDKALNLIGKKFKELNLTNIYAPPLPSLALPSLPMTSWLMLPDGITVEVIVVNQVNAGHLFVQQHTHPTFHALRSLDQQMYLCYSQPGIPTLPTPVEITVICAAPGADGAWWRAQVVASYEETNEVEIRYVDYGGYKRVKVDVLRQIRSDFVTLPFQGAEVLLDSVMPLSDDDQFSPEADAAMSEMTGNTALLAQVTSYSPTGLPLIQLWSVVGDEVVLINRSLVERGLAQWVDSYYTSL |
| 预测分子量 | 97,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于AKAP1重组蛋白的参考文献概览：
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1. **"A-kinase anchoring protein 1 (AKAP1) regulates mitochondrial oxidative metabolism and cristae structure through modulation of Drp1 fragmentation"**
- **作者**: G. Merrill et al.
- **摘要**: 本研究通过重组AKAP1蛋白的体外实验，揭示了AKAP1通过结合动力相关蛋白Drp1调控线粒体分裂的机制。研究证明，AKAP1的N端结构域重组蛋白可抑制Drp1活性，从而维持线粒体嵴结构完整性，并影响细胞能量代谢。
2. **"AKAP1 recruits PKA to lipid droplets through a conserved amphipathic helix"**
- **作者**: E. Lundquist et al. (2021)
- **摘要**: 作者利用重组AKAP1蛋白的脂滴靶向结构域(含两性α螺旋)，阐明了AKAP1将蛋白激酶A(PKA)锚定至脂滴表面的分子机制，并发现该过程对脂肪分解的时空调控至关重要。
3. **"Structural basis for AKAP1-mediated regulation of mitochondrial PKA signaling"**
- **作者**: C. Smith et al. (2018)
- **摘要**: 通过重组AKAP1蛋白的晶体结构分析，揭示了其C端PKA结合域与激酶调控亚基的相互作用界面，提出了AKAP1在线粒体PKA信号转导中的构象变化模型，为相关疾病治疗提供靶点。
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以上文献均涉及AKAP1重组蛋白的功能或结构研究，涵盖线粒体调控、脂代谢及结构生物学领域。如需具体文献链接或补充，可进一步提供数据库检索支持。
A-Kinase Anchoring Protein 1 (AKAP1), also known as Dual Specificity AKAP1 or D-AKAP1. is a scaffold protein that anchors protein kinase A (PKA) and other signaling molecules to specific subcellular compartments, facilitating localized signal transduction. It belongs to the AKAP family, which plays critical roles in organizing spatial-temporal signaling by tethering PKA to membranes, organelles, or cytoskeletal structures. AKAP1 is predominantly localized to mitochondria and the endoplasmic reticulum via its N-terminal targeting domain, which includes a mitochondrial transmembrane domain and a peroxisomal targeting signal. Its C-terminal region contains a PKA-binding domain and RNA-binding motifs (e.g., KH domains), enabling interactions with mRNA and regulatory RNAs.
Functionally, AKAP1 regulates mitochondrial dynamics, apoptosis, metabolism, and oxidative stress responses by modulating PKA-mediated phosphorylation of downstream targets. It also participates in cellular adaptation to hypoxia and metabolic stress. Dysregulation of AKAP1 has been implicated in diseases such as cancer, cardiovascular disorders, and neurodegenerative conditions, highlighting its therapeutic potential.
Recombinant AKAP1 proteins are engineered to study its structure-function relationships, binding partners, and signaling roles in vitro. These proteins are typically produced using bacterial (e.g., E. coli) or mammalian expression systems, often fused with tags (e.g., His, GST) for purification and detection. Recombinant AKAP1 serves as a tool to investigate PKA anchoring mechanisms, mitochondrial-nuclear crosstalk, and its role in stress adaptation pathways. Researchers also utilize it to screen for inhibitors or stabilizers of AKAP1-mediated complexes, aiming to develop targeted therapies for AKAP1-associated pathologies.
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