| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAL1 |
| Uniprot No | P17542 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-331aa |
| 氨基酸序列 | MTERPPSEAA RSDPQLEGRD AAEASMAPPH LVLLNGVAKE TSRAAAAEPP VIELGARGGP GGGPAGGGGA ARDLKGRDAA TAEARHRVPT TELCRPPGPA PAPAPASVTA ELPGDGRMVQ LSPPALAAPA APGRALLYSL SQPLASLGSG FFGEPDAFPM FTTNNRVKRR PSPYEMEITD GPHTKVVRRI FTNSRERWRQ QNVNGAFAEL RKLIPTHPPD KKLSKNEILR LAMKYINFLA KLLNDQEEEG TQRAKTGKDP VVGAGGGGGG GGGGAPPDDL LQDVLSPNSS CGSSLDGAAS PDSYTEEPAP KHTARSLHPA MLPAADGAGP R |
| 预测分子量 | 34,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于TAL1重组蛋白的参考文献摘要：
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1. **文献名称**: "Production and functional characterization of recombinant TAL1 transcription factor in Escherichia coli"
**作者**: Smith J, et al.
**摘要**: 本研究报道了在大肠杆菌中高效表达并纯化具有生物活性的重组TAL1蛋白。通过优化密码子使用和诱导条件，获得可溶性蛋白，并验证其与DNA靶序列的结合能力，为白血病机制研究提供工具。
2. **文献名称**: "Structural insights into TAL1-DNA complex formation using recombinant protein technology"
**作者**: Lee S, et al.
**摘要**: 利用昆虫细胞系统表达重组TAL1蛋白，结合X射线晶体学解析其与DNA复合物的三维结构，揭示了TAL1特异性识别DNA的分子机制，为靶向治疗设计奠定基础。
3. **文献名称**: "Recombinant TAL1 protein rescues hematopoietic defects in a zebrafish knockout model"
**作者**: Chen H, et al.
**摘要**: 通过体外表达重组TAL1蛋白并注射至斑马鱼基因敲除模型，证明其能恢复造血干细胞分化功能，验证了TAL1在发育中的关键作用及重组蛋白的功能活性。
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注：以上为模拟文献，实际引用时需根据具体论文内容调整。建议通过PubMed或Web of Science以关键词“TAL1 recombinant protein”检索最新研究。
**Background of TAL1 Recombinant Protein**
TAL1 (T-cell acute lymphocytic leukemia protein 1), also known as SCL (stem cell leukemia) or TAL-1. is a critical transcription factor belonging to the basic helix-loop-helix (bHLH) family. It plays a pivotal role in hematopoietic development, particularly in the differentiation of erythroid, megakaryocytic, and endothelial lineages. Discovered through its involvement in chromosomal translocations in T-cell acute lymphoblastic leukemia (T-ALL), TAL1 regulates gene expression by forming complexes with other transcription factors (e.g., E-proteins, GATA-1/2) and binding to specific DNA sequences, often at E-box motifs.
The recombinant TAL1 protein is engineered using molecular cloning techniques, typically expressed in bacterial (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells), ensuring proper post-translational modifications for functional studies. Purification methods like affinity chromatography (e.g., His-tag, GST-tag) yield high-purity protein for research applications. Recombinant TAL1 is widely used to study hematopoiesis, angiogenesis, and leukemogenesis. It aids in elucidating mechanisms of transcriptional regulation, protein-DNA/co-factor interactions, and dysregulation in cancers.
Additionally, TAL1 recombinant protein serves as a tool for drug screening, aiming to identify inhibitors that disrupt oncogenic TAL1 complexes in T-ALL. Its role in stem cell biology and regenerative medicine further highlights its therapeutic relevance. By enabling *in vitro* and *in vivo* functional assays, recombinant TAL1 continues to advance our understanding of developmental biology and disease pathology.
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