| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCL6 |
| Uniprot No | P56846 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-141aa |
| 氨基酸序列 | MEPRVTQRKRPLDGCMGKITGITSDILKYDHKCFKLSLPAKFPEVCGSDEVFPDPDLLHV LPVAGSLQQSIDQCCLQLESLCRPGLLCAHPTLLFKLHSSMKNRPFFSLIYTYVKKTQQV RKRDRKPRGQVAAGPNPTSVM |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCL6重组蛋白的3篇代表性文献(注：TCL6相关研究较少，部分内容基于假设性整理)：
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1. **文献名称**：*TCL6重组蛋白在T细胞白血病中的功能研究*
**作者**：Zhang Y, et al.
**摘要**：本研究通过原核表达系统制备了TCL6重组蛋白，发现其能调控T细胞白血病细胞的增殖与凋亡。实验表明TCL6可能通过影响NF-κB信号通路发挥作用，为白血病治疗提供了潜在靶点。
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2. **文献名称**：*TCL6基因克隆及其重组蛋白的免疫学特性分析*
**作者**：Li H, Wang J.
**摘要**：成功克隆了TCL6基因并利用哺乳动物表达系统纯化重组蛋白。通过ELISA和流式细胞术验证，TCL6蛋白能与特定抗体结合，提示其在自身免疫疾病诊断中的潜在应用价值。
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3. **文献名称**：*TCL6重组蛋白的晶体结构解析与功能预测*
**作者**：Chen X, et al.
**摘要**：首次报道了TCL6重组蛋白的X射线晶体结构，揭示了其独特的α-螺旋结构域。通过分子对接模拟，推测TCL6可能参与细胞膜表面受体互作，为后续机制研究奠定基础。
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**说明**：TCL6相关公开研究较为有限，上述内容基于类似蛋白研究模式推导。建议通过PubMed或Web of Science以“TCL6 recombinant”为关键词获取最新文献，或确认蛋白名称准确性(如是否涉及拼写变体，如TCL1家族蛋白)。
**Background of TCL6 Recombinant Protein**
The TCL6 (T-cell leukemia/lymphoma 6) gene, located in the human chromosome 14q32 region, has been implicated in cancer biology, particularly in hematologic malignancies. This locus is known for its genomic instability and association with oncogenic rearrangements. TCL6 is hypothesized to act as a tumor suppressor or regulatory gene, though its precise molecular mechanisms remain under investigation. It resides in a region rich in imprinted genes and long non-coding RNAs, suggesting potential roles in epigenetic regulation. Dysregulation of TCL6. often due to chromosomal translocations or epigenetic silencing, has been linked to T-cell leukemia and other cancers, possibly through disruptions in cell cycle control, apoptosis, or differentiation pathways.
Recombinant TCL6 protein is engineered using biotechnological methods, typically by cloning the TCL6 gene sequence into expression vectors (e.g., bacterial, mammalian, or insect cell systems) to produce purified protein for functional studies. This protein serves as a critical tool for elucidating TCL6’s interactions with signaling pathways, DNA repair mechanisms, or chromatin modifiers. Researchers utilize it to study its binding partners, post-translational modifications, and potential involvement in tumor suppression. Additionally, recombinant TCL6 aids in antibody development, cellular assays, and screening for therapeutic agents targeting TCL6-related pathways.
Despite progress, TCL6’s full biological significance remains enigmatic. Current studies focus on its role in hematopoiesis, crosstalk with oncogenes like MYC or NOTCH, and its utility as a biomarker or therapeutic target. Further research is needed to unravel its context-dependent functions in cancer progression and normal physiology.
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