纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | DOCK10 |
Uniprot No | Q96BY6 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 全长 |
氨基酸序列 | full |
预测分子量 | 249 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOCK10重组蛋白的3篇参考文献示例(注:文献为虚构示例,仅供格式参考):
1. **"DOCK10 Recombinant Protein Promotes Melanoma Cell Migration via Cdc42 Activation"**
- **作者**: Garcia, R. et al.
- **摘要**: 研究通过表达DOCK10重组蛋白,证明其通过激活Cdc42调控黑色素瘤细胞迁移,揭示了DOCK10在肿瘤转移中的潜在作用。
2. **"Structural and Functional Analysis of DOCK10 Guanine Exchange Activity"**
- **作者**: Chen, L. & Wang, H.
- **摘要**: 利用重组DOCK10蛋白解析其GEF(鸟苷酸交换因子)结构域,证实其对RhoGTPase家族成员的特异性结合及激活机制。
3. **"DOCK10 Recombinant Protein Modulates B-cell Receptor Signaling in Autoimmune Disorders"**
- **作者**: Saito, M. et al.
- **摘要**: 体外实验表明,DOCK10重组蛋白通过调控B细胞受体信号通路,影响自身免疫性疾病中淋巴细胞的异常活化。
4. **"DOCK10 Overexpression via Recombinant Protein Delivery Suppresses Hepatocellular Carcinoma Invasion"**
- **作者**: Lee, J. et al.
- **摘要**: 研究发现递送DOCK10重组蛋白可抑制肝癌细胞侵袭,机制涉及整合素信号通路的下调,提示其作为治疗靶点的潜力。
(注:以上文献为模拟内容,实际研究中请通过PubMed或Web of Science等平台检索具体文献。)
DOCK10. a member of the Dedicator of Cytokinesis (DOCK) protein family, is a guanine nucleotide exchange factor (GEF) that specifically activates Rho GTPases such as Cdc42 and Rac1. These GTPases regulate cytoskeletal dynamics, cell migration, and intracellular signaling. The DOCK family is divided into four subfamilies (DOCK-A to DOCK-D), with DOCK10 belonging to the DOCK-D subfamily. Unlike other DOCK proteins, DOCK10 contains a unique C-terminal CZH2 domain, which facilitates interactions with membrane-associated proteins and GTPase activation. It is predominantly expressed in immune cells (e.g., B lymphocytes) and epithelial tissues, suggesting roles in immune regulation, tissue development, and cancer progression. Studies link DOCK10 to B-cell receptor signaling, lymphocyte trafficking, and tumor metastasis, particularly in hematological malignancies and solid tumors.
Recombinant DOCK10 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce functional domains or full-length protein for research. It enables in vitro studies on GTPase binding, GEF activity, and signaling mechanisms. Purification often involves affinity tags (e.g., GST, His-tag) followed by biochemical validation (e.g., SDS-PAGE, Western blot). Researchers utilize recombinant DOCK10 to investigate its role in diseases, screen inhibitors, or develop diagnostic tools. Its dysregulation in cancers and immune disorders highlights its potential as a therapeutic target. However, structural complexity and post-translational modifications pose challenges in producing active recombinant forms, necessitating optimized expression strategies. Current research focuses on elucidating DOCK10’s molecular interactions and therapeutic applications in precision medicine.
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