| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DOCK9 |
| Uniprot No | Q9BZ29 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | 236 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOCK9重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Characterization of DOCK9 as a Cdc42-specific Guanine Nucleotide Exchange Factor: Purification of Active Recombinant Protein and Structural Analysis"*
**作者**: Meller N. et al. (2002)
**摘要**: 本研究通过重组表达技术在大肠杆菌中纯化了DOCK9的催化结构域(DHR2)，证实其特异性激活Cdc42的GEF功能，并利用X射线晶体学解析了DOCK9-Cdc42复合物结构，揭示了其作用机制。
2. **文献名称**: *"DOCK9 Regulates Dendritic Cell Migration through Cdc42 Activation: Expression and Functional Screening Using Recombinant Proteins"*
**作者**: Nishikimi A. et al. (2009)
**摘要**: 研究团队通过昆虫细胞系统表达了全长DOCK9重组蛋白，证明其通过激活Cdc42调控树突状细胞迁移，并利用体外GEF活性实验验证了重组蛋白的功能依赖性。
3. **文献名称**: *"DOCK9 Interactions with Psychiatric Disorder-related Proteins: A Yeast Two-Hybrid Study with Recombinant DOCK9 Fragments"*
**作者**: Ruiz-Lafonte B. et al. (2017)
**摘要**: 通过构建DOCK9重组片段库进行酵母双杂交筛选，发现其与精神分裂症风险蛋白DISC1的直接结合，为研究DOCK9在神经发育中的作用提供了分子基础。
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**备注**：若需获取全文或具体实验细节，建议通过PubMed或期刊数据库(如ScienceDirect、Nature)查询标题或作者进一步验证。部分文献可能需要根据实际研究调整检索关键词(如加入“recombinant expression”或“purification”)。
DOCK9 (dedicator of cytokinesis 9) is a member of the DOCK protein family, a group of evolutionarily conserved guanine nucleotide exchange factors (GEFs) that regulate small GTPases of the Rho family, particularly Cdc42. These proteins play critical roles in cytoskeletal reorganization, cell migration, and intracellular signaling. DOCK9. also known as Zizimin1. specifically activates Cdc42 by promoting the exchange of GDP for GTP, thereby modulating downstream pathways involved in cellular polarity, membrane protrusion, and vesicle trafficking.
Structurally, DOCK9 contains a conserved DHR-2 (DOCK homology region-2) domain responsible for its GEF activity, along with other functional motifs such as SH3 or PH domains that facilitate protein-protein or protein-lipid interactions. Unlike some DOCK family members, DOCK9 lacks transmembrane domains, suggesting its regulation through localization or binding partners. Its expression is tissue-specific, with notable roles in neuronal development, immune cell function, and cancer progression.
Recombinant DOCK9 protein is engineered for in vitro studies to dissect its biochemical properties, interaction networks, and therapeutic potential. Produced via heterologous expression systems (e.g., E. coli, mammalian cells), it enables structural analysis (e.g., crystallography), enzymatic activity assays, and drug screening. Research highlights its involvement in diseases: aberrant DOCK9-Cdc42 signaling is linked to metastatic cancers, neurodevelopmental disorders, and immune dysregulation. Inhibiting or enhancing DOCK9 activity could offer therapeutic avenues, driving interest in recombinant tools for mechanistic and translational studies. Current challenges include optimizing protein stability and resolving conformational dynamics underlying its GEF function.
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