| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PNCK |
| Uniprot No | Q6P2M8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-343aa |
| 氨基酸序列 | MLLLKKHTED ISSVYEIRER LGSGAFSEVV LAQERGSAHL VALKCIPKKA LRGKEALVEN EIAVLRRISH PNIVALEDVH ESPSHLYLAM ELVTGGELFD RIMERGSYTE KDASHLVGQV LGAVSYLHSL GIVHRDLKPE NLLYATPFED SKIMVSDFGL SKIQAGNMLG TACGTPGYVA PELLEQKPYG KAVDVWALGV ISYILLCGYP PFYDESDPEL FSQILRASYE FDSPFWDDIS ESAKDFIRHL LERDPQKRFT CQQALRHLWI SGDTAFDRDI LGSVSEQIRK NFARTHWKRA FNATSFLRHI RKLGQIPEGE GASEQGMARH SHSGLRAGQP PKW |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PNCK重组蛋白的示例性参考文献(注：部分内容可能为模拟虚构，实际文献需通过学术数据库查询)：
1. **文献名称**: "Recombinant PNCK promotes cell proliferation in breast cancer via calcium signaling pathways"
**作者**: Smith A, et al.
**摘要**: 研究利用重组PNCK蛋白分析了其在乳腺癌细胞增殖中的作用，发现PNCK通过激活钙调蛋白依赖性激酶通路，促进肿瘤细胞生长，为靶向治疗提供了潜在方向。
2. **文献名称**: "Expression and purification of functional PNCK in Escherichia coli for neurodegenerative disease studies"
**作者**: Johnson R, et al.
**摘要**: 报道了一种在大肠杆菌中高效表达并纯化功能性PNCK重组蛋白的方法，并验证其在体外模型中调节神经元钙稳态的能力，为神经退行性疾病机制研究提供工具。
3. **文献名称**: "Structural insights into PNCK kinase domain by X-ray crystallography using recombinant protein"
**作者**: Lee S, et al.
**摘要**: 通过重组PNCK蛋白的晶体结构解析，揭示了其激酶结构域与钙调蛋白结合的分子机制，为设计特异性抑制剂奠定结构基础。
4. **文献名称**: "PNCK recombinant protein modulates trophoblast invasion in early pregnancy"
**作者**: Brown K, et al.
**摘要**: 研究证明重组PNCK蛋白通过调控滋养层细胞的迁移和侵袭能力，影响胚胎着床过程，提示其在妊娠相关疾病中的潜在作用。
**注意**：以上文献信息为示例性质，实际研究中请通过PubMed、Web of Science等平台检索具体文献。若需真实文献，建议使用关键词“PNCK recombinant protein”或“PNCK kinase expression”进行精确查询。
**Background of PNCK Recombinant Protein**
PNCK (Pregnancy-Upregulated Nonubiquitous Calmodulin Kinase) is a calcium/calmodulin-dependent protein kinase encoded by the *PNCK* gene, located on human chromosome Xp11.23. It belongs to the Ca²⁺/calmodulin-dependent kinase (CaMK) family, which plays critical roles in calcium-mediated signaling pathways. PNCK is uniquely regulated during pregnancy, showing upregulated expression in placental and uterine tissues, suggesting its involvement in gestational processes such as trophoblast differentiation or maternal-fetal communication.
Structurally, PNCK contains a catalytic kinase domain and a calmodulin-binding regulatory domain, enabling its activation by calcium signaling. Beyond pregnancy, PNCK is expressed in various tissues, including the brain, kidney, and cancer cells. Studies highlight its dual role in cell survival and apoptosis, depending on cellular context. For instance, PNCK promotes apoptosis in renal cells under stress but supports survival in certain cancer types by modulating pathways like p53 or ERK/MAPK. Dysregulation of PNCK has been linked to cancers (e.g., breast, glioblastoma) and neurological disorders, positioning it as a potential therapeutic target.
Recombinant PNCK protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its purified form enables in vitro analysis of kinase activity, substrate interactions, and inhibitor screening. Researchers utilize it to decipher PNCK's role in calcium signaling, disease mechanisms, and gestational biology, offering insights for drug development or diagnostic biomarkers. Ongoing research aims to clarify its tissue-specific functions and therapeutic applicability.
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