| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BCAS2 |
| Uniprot No | O75934 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-225aa |
| 氨基酸序列 | AGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLS YLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDIT AWQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQK ELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQ LENEIYQIKQQHGEANKENIRQDF |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BCAS2重组蛋白的3篇参考文献及其摘要内容概要：
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1. **文献名称**: *BCAS2 regulates androgen receptor-mediated gene regulation via interaction with PVIP*
**作者**: Lee YM et al.
**摘要**: 研究报道了重组BCAS2蛋白在体外与雄激素受体(AR)和PVIP蛋白的相互作用，揭示了BCAS2通过调控AR的转录活性参与前列腺癌进展的分子机制。
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2. **文献名称**: *Functional characterization of BCAS2 in pre-mRNA splicing and cell proliferation*
**作者**: Wang Q et al.
**摘要**: 通过表达纯化重组BCAS2蛋白，发现其直接结合剪接体核心组分SF3B1.并证明BCAS2通过调控前体mRNA剪接影响细胞周期和肿瘤细胞增殖。
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3. **文献名称**: *Structural insights into BCAS2 role in the BRCA1-A complex*
**作者**: Zhang L et al.
**摘要**: 利用重组BCAS2蛋白进行晶体结构解析，阐明了其与BRCA1-A复合物其他成员的结合界面，为BCAS2在DNA损伤修复中的功能提供结构基础。
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以上文献均涉及重组BCAS2蛋白的制备及其在剪接调控、癌症信号通路或DNA修复中的功能研究。
**Background of BCAS2 Recombinant Protein**
BCAS2 (Breast Carcinoma Amplified Sequence 2), also known as SPF27 or CSNK1D-binding protein, is a multifunctional protein encoded by the *BCAS2* gene located on human chromosome 1p22.3. It plays critical roles in pre-mRNA splicing as a component of the spliceosome, particularly within the Prp19/CDC5L complex, and is implicated in maintaining genomic stability through DNA repair pathways. BCAS2 interacts with various partners, including CK1δ (CSNK1D) and the androgen receptor (AR), influencing cellular processes like cell cycle regulation, apoptosis, and hormone signaling.
Dysregulation of BCAS2 is linked to cancer progression, notably breast cancer, where its overexpression correlates with tumor aggressiveness and poor prognosis. It also contributes to neurodegenerative diseases, such as spinal muscular atrophy, by affecting splicing efficiency. To study its molecular mechanisms, BCAS2 recombinant protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) and purified via affinity tags (e.g., His-tag). This recombinant tool enables *in vitro* studies on protein-protein interactions, splicing assays, and drug screening.
Research on BCAS2 recombinant protein is pivotal for unraveling its role in disease pathways and developing targeted therapies. Its application extends to exploring splicing-related disorders and cancer biomarkers, highlighting its potential as a diagnostic or therapeutic target.
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