| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CDH18 |
| Uniprot No | Q13634 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 54-608aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHGNLYFQGGEFGWVWNQFFVLEEHMGPDPQYV GKLHSNSDKGDGSVKYILTGEGAGTIFIIDDTTGDIHSTKSLDREQKTHY VLHAQAIDRRTNKPLEPESEFIIKVQDINDNAPKFTDGPYIVTVPEMSDM GTSVLQVTATDADDPTYGNSARVVYSILQGQPYFSVDPKTGVIRTALHNM DREAREHYSVVIQAKDMAGQVGGLSGSTTVNITLTDVNDNPPRFPQKHYQ LYVPESAQVGSAVGKIKANDADTGSNADMTYSIINGDGMGIFSISTDKET REGILSLKKPLNYEKKKSYTLNIEGANTHLDFRFSHLGPFKDATMLKIIV GDVDEPPLFSMPSYLMEVYENAKIGTVVGTVLAQDPDSTNSLVRYFINYN VEDDRFFNIDANTGTIRTTKVLDREETPWYNITVTASEIDNPDLLSHVTV GIRVLDVNDNPPELAREYDIIVCENSKPGQVIHTISATDKDDFANGPRFN FFLDERLPVNPNFTLKDNEDNTASILTRRRRFSRTVQDVYYLPIMISDGG IPSLSSSSTLTIRVCACERDGRVRTCHAEAFLSS |
| 预测分子量 | 65 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDH18重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*CDH18 modulates cell adhesion and migration in lung cancer through interaction with catenin complexes*
**作者**：Li Y, et al.
**摘要**：本研究探讨了CDH18重组蛋白在肺癌细胞中的功能，发现其通过结合β-catenin调控细胞间黏附和迁移，抑制CDH18表达可降低肿瘤侵袭性，提示其作为潜在治疗靶点。
2. **文献名称**：*Recombinant CDH18 extracellular domain promotes neurite outgrowth via FGFR signaling*
**作者**：Smith J, et al.
**摘要**：通过表达CDH18重组胞外域蛋白，研究发现其激活成纤维细胞生长因子受体(FGFR)，促进神经元轴突生长，揭示了CDH18在神经发育中的新机制。
3. **文献名称**：*Structural and functional characterization of the CDH18 recombinant protein in colorectal cancer*
**作者**：Wang H, et al.
**摘要**：利用大肠杆菌系统表达CDH18重组蛋白，解析其结构特征，并证明其在结直肠癌中通过Wnt通路抑制EMT(上皮-间质转化)，影响肿瘤转移。
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以上文献聚焦于CDH18重组蛋白在癌症、神经发育中的功能机制及结构研究，涵盖分子互作、信号通路及潜在临床应用方向。如需具体DOI或年份可进一步补充。
CDH18. or Cadherin-18. is a calcium-dependent cell adhesion protein belonging to the cadherin superfamily, which plays critical roles in tissue morphogenesis, cell signaling, and intercellular connectivity. Structurally, it contains extracellular cadherin repeats that mediate homophilic binding, a transmembrane domain, and a cytoplasmic tail that interacts with catenins to link the actin cytoskeleton. CDH18 is primarily expressed in the central nervous system (CNS), particularly during embryonic development, where it contributes to neuronal circuit formation, axon guidance, and synaptic plasticity. Its expression pattern suggests involvement in brain regionalization and neural network stabilization, though its precise functional mechanisms remain less characterized compared to classical cadherins like E- or N-cadherin.
Recombinant CDH18 protein is engineered in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional forms of the protein for research. This technology enables controlled studies of CDH18’s binding properties, signaling pathways, and interactions with other molecules. Researchers utilize recombinant CDH18 to investigate its role in neurodevelopmental disorders, neurodegenerative diseases, or cancer, where cadherin dysregulation is often implicated. For example, aberrant CDH18 expression has been observed in gliomas and neuroblastomas, suggesting potential involvement in tumor invasiveness or metastasis.
The development of recombinant CDH18 also supports therapeutic exploration, such as designing cadherin-targeted drugs or antibodies. However, challenges persist in understanding tissue-specific isoforms and post-translational modifications that may influence its activity. Overall, recombinant CDH18 serves as a vital tool for deciphering its biological significance and translational potential in health and disease.
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