| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX7 |
| Uniprot No | Q9BT81 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-388aa |
| 氨基酸序列 | MASLLGAYPWPEGLECPALDAELSDGQSPPAVPRPPGDKGSESRIRRPMN AFMVWAKDERKRLAVQNPDLHNAELSKMLGKSWKALTLSQKRPYVDEAER LRLQHMQDYPNYKYRPRRKKQAKRLCKRVDPGFLLSSLSRDQNALPEKRS GSRGALGEKEDRGEYSPGTALPSLRGCYHEGPAGGGGGGTPSSVDTYPYG LPTPPEMSPLDVLEPEQTFFSSPCQEEHGHPRRIPHLPGHPYSPEYAPSP LHCSHPLGSLALGQSPGVSMMSPVPGCPPSPAYYSPATYHPLHSNLQAHL GQLSPPPEHPGFDALDQLSQVELLGDMDRNEFDQYLNTPGHPDSATGAMA LSGHVPVSQVTPTGPTETSLISVLADATATYYNSYSVS |
| 预测分子量 | 69 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SOX7重组蛋白的3-4篇参考文献示例(基于公开领域研究整理，非真实引用，仅供参考)：
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1. **文献名称**：*SOX7 regulates Wnt/β-catenin signaling pathway by directly binding to β-catenin in colorectal cancer*
**作者**：Li Y, et al.
**摘要**：研究利用重组SOX7蛋白进行体外结合实验，证明SOX7通过与β-catenin直接互作抑制Wnt信号通路活性，进而抑制结直肠癌细胞增殖和迁移。
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2. **文献名称**：*Functional analysis of SOX7 domains in embryonic stem cell differentiation*
**作者**：Smith J, et al.
**摘要**：通过重组SOX7蛋白的截断体实验，发现其HMG结构域对调控中胚层分化至关重要，并证实其重组蛋白可诱导小鼠胚胎干细胞向心肌细胞定向分化。
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3. **文献名称**：*SOX7 as a tumor suppressor: Recombinant SOX7 protein inhibits angiogenesis in vitro*
**作者**：Chen H, et al.
**摘要**：研究表明，重组SOX7蛋白可抑制血管内皮细胞管腔形成，通过下调VEGF信号通路，提示其在抑制肿瘤血管生成中的潜在治疗价值。
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4. **文献名称**：*Structural characterization and functional validation of recombinant SOX7 in hematopoietic development*
**作者**：Wang R, et al.
**摘要**：研究成功表达并纯化重组人SOX7蛋白，通过斑马鱼模型验证其调控造血干细胞分化的功能，揭示其在血液系统发育中的关键作用。
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注：以上为模拟文献，实际引用请通过PubMed、Web of Science等平台检索真实发表论文。
SOX7 recombinant protein is a purified, biologically active form of the SOX7 transcription factor, engineered through recombinant DNA technology. Belonging to the SOX (SRY-related HMG-box) protein family, SOX7 contains a conserved high-mobility group (HMG) DNA-binding domain that enables sequence-specific interactions with regulatory regions of target genes. It plays critical roles in embryonic development, cell differentiation, and tissue homeostasis, particularly in cardiovascular formation, endoderm specification, and hematopoietic stem cell regulation. SOX7 is also implicated in tumor suppression, where it antagonizes oncogenic pathways like Wnt/β-catenin by disrupting β-catenin/TCF complexes or promoting its degradation.
Recombinant SOX7 is typically produced in heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and consistent activity. The protein often includes affinity tags (e.g., His-tag) for simplified purification and detection. Post-translational modifications, such as phosphorylation, may be preserved in eukaryotic expression systems to maintain native functionality. Quality control involves SDS-PAGE, Western blotting, and functional assays (e.g., DNA-binding ELISAs or luciferase reporter systems) to confirm structural integrity and bioactivity.
In research, SOX7 recombinant protein is widely used to study gene regulatory networks, model developmental processes, and investigate diseases like cancer or congenital disorders. It serves as a tool to rescue SOX7-deficient cellular phenotypes, modulate Wnt signaling in cancer models, or differentiate stem cells into specific lineages. Its therapeutic potential is being explored in regenerative medicine and targeted cancer therapies. However, challenges remain in optimizing delivery methods and understanding context-dependent interactions with other SOX family members (e.g., SOX17. SOX18) in complex biological systems.
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