| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INSM2 |
| Uniprot No | Q96T92 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-566aa |
| 氨基酸序列 | MPRGFLVKRT KRTGGLYRVR LAERVFPLLG PQGAPPFLEE APSASLPGAE RATPPTREEP GKGLTAEAAR EQSGSPCRAA GVSPGTGGRE GAEWRAGGRE GPGPSPSPSP SPAKPAGAEL RRAFLERCLS SPVSAESFPG GAAAVAAFSC SVAPAAAPTP GEQFLLPLRA PFPEPALQPD PAPLSAALQS LKRAAGGERR GKAPTDCASG PAAAGIKKPK AMRKLSFADE VTTSPVLGLK IKEEEPGAPS RGLGGSRTPL GEFICQLCKE QYADPFALAQ HRCSRIVRVE YRCPECDKVF SCPANLASHR RWHKPRPAAA NAATVSSADG KPPSSSSSSS RDSGAIASFL AEGKENSRIE RTADQHPQAR DSSGADQHPD SAPRQGLQVL THPEPPLPQG PYTEGVLGRR VPVPGSTSGG RGSEIFVCPY CHKKFRRQAY LRKHLSTHEA GSARALAPGF GSERGAPLAF ACPLCGAHFP TADIREKHRL WHAVREELLL PALAGAPPET SGPSGPSDGS AQQIFSCKHC PSTFFSSPGL TRHINKCHPS ESRQVLLLQM PLRPGC |
| 预测分子量 | 59,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于INSM2重组蛋白的模拟参考文献示例(注：INSM2相关研究较为有限，以下内容为假设性示例，建议通过学术数据库核实最新文献)：
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1. **文献名称**: *"Expression and Functional Characterization of Recombinant INSM2 in Neuroendocrine Differentiation"*
**作者**: Zhang L, et al.
**摘要**: 本研究通过大肠杆菌系统表达了重组INSM2蛋白，并验证其在神经内分泌细胞分化中的转录调控作用。结果表明，INSM2重组蛋白可激活下游靶基因，促进细胞分化。
2. **文献名称**: *"INSM2 Recombinant Protein Purification and Its Role in Pancreatic Beta-Cell Regeneration"*
**作者**: Gupta S, et al.
**摘要**: 利用哺乳动物表达系统纯化INSM2重组蛋白，发现其能增强胰腺β细胞的增殖能力，为糖尿病治疗提供潜在分子工具。
3. **文献名称**: *"Structural Analysis of INSM2 Zinc-Finger Domain and DNA Binding Activity"*
**作者**: Müller R, et al.
**摘要**: 解析了INSM2重组蛋白的锌指结构域晶体结构，揭示其特异性结合DNA的分子机制，为靶向药物设计奠定基础。
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**建议**：如需真实文献，可通过PubMed、Web of Science等平台，以关键词“INSM2 recombinant protein”或“INSM2 expression”检索，并筛选近5年研究。注意确认基因名称拼写准确性(如是否与INSM1混淆)。
INSM2 (Insulinoma-associated protein 2) is a zinc-finger transcription factor encoded by the *INSM2* gene, belonging to the INSM family of proteins. Initially identified through its homology to INSM1. a well-characterized marker of neuroendocrine tumors and neural development, INSM2 shares structural features such as conserved zinc-finger DNA-binding domains that enable sequence-specific interactions with gene promoters. While INSM1 is extensively studied in neuroendocrine differentiation and cancers, INSM2 remains less characterized, though emerging evidence suggests its involvement in cellular proliferation, differentiation, and tumorigenesis.
The recombinant INSM2 protein is engineered using biotechnological platforms (e.g., bacterial or mammalian expression systems) to produce purified, functional protein for research and therapeutic applications. Its recombinant form typically retains the DNA-binding capacity and regulatory functions of the native protein, allowing scientists to study its role in gene regulation, particularly in developmental biology and cancer contexts. Studies suggest INSM2 may influence pathways critical to neuroendocrine cell development and tumor progression, making it a potential biomarker or therapeutic target.
Research on recombinant INSM2 also explores its utility in elucidating mechanisms of transcriptional repression or activation, given its ability to modulate downstream genes. Additionally, it serves as an antigen for antibody production in diagnostic assays. Despite its emerging significance, further studies are needed to fully unravel its physiological and pathological roles, highlighting the importance of recombinant INSM2 as a tool for advancing molecular and clinical research.
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