| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | CDC14 |
| Uniprot No | Q59NH8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-542aa |
| 氨基酸序列 | MHSSSVHVPLIEFLKNRIYLGAYDHHKRDTEDLAYFTVEDALPYNAFYMDFGPLNIGHLYRFAVLLHKKLNEDSTQGKGLVIYSSTSPKERANLACLLCCYMILLQNWAPHQVLQPIAQITPPLQAFRDAGYSSADYEITIQDVVYAMWRAKERGMIDLAKFDLDEYEQYERVDQGDFNVISKDFIAFASPQQSKRGGLNEPFQKVLEYFVENNVQLVVRLNSHLYDAKEFTKRNIKHIDMIFDDGTCPTLEYVQKFIGAAECIINKGGKIAVHCKAGLGRTGCLIGAHLIYTHGFTANECIAYMRMIRPGMVVGPQQHWLYLHHDDFRSWRHTMIVDNRPDPLIGNLFPLCSYEDYKQRLKEAKRKERLQLQQQLTSPLADSSVINTPIRRRKVSGALASKIQTVVPIESPGQPRKYFEDSEDIDEVEMVNNSDDENTMQDIIQSSPARYDSVTPKTKDNSDWRVLRSISTNNVSSQQSIHIIKTTTTKTVNETLSSPPGTSPTNVLRVSKARSKNRIASGNSQTSRAHSGGVRKLSGKKH |
| 预测分子量 | 61,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDC14重组蛋白的3篇参考文献概览(信息基于模拟生成，非真实文献)：
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1. **文献名称**：*Purification and functional analysis of recombinant Cdc14 phosphatase from Saccharomyces cerevisiae*
**作者**：Smith J., et al.
**摘要**：该研究通过在大肠杆菌中表达并纯化重组酵母Cdc14蛋白，验证了其磷酸酶活性及对特定细胞周期蛋白底物的去磷酸化功能，揭示了其在退出有丝分裂中的调控机制。
2. **文献名称**：*Structural insights into human Cdc14A phosphatase using recombinant protein crystallography*
**作者**：Li X., Zhang R.
**摘要**：利用重组表达的人源Cdc14A蛋白进行结晶和结构解析，揭示了其催化结构域的关键氨基酸残基及其与底物结合的分子机制，为靶向药物设计提供结构基础。
3. **文献名称**：*Role of recombinant Cdc14B in DNA damage response: Evidence from in vitro assays*
**作者**：Wang Y., et al.
**摘要**：通过体外实验证明，重组Cdc14B蛋白参与DNA损伤修复通路，可调控Chk1激酶的活性，提示其在维持基因组稳定性中的潜在作用。
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如需真实文献，建议通过PubMed或Google Scholar检索关键词(如“Cdc14 recombinant protein phosphatase”)，并筛选近年的高影响力研究。
**Background of CDC14 Recombinant Protein**
Cdc14. a conserved dual-specificity phosphatase, plays a pivotal role in regulating cell cycle progression, particularly during exit from mitosis and DNA damage response. Initially identified in *Saccharomyces cerevisiae*, Cdc14 homologs have been characterized in eukaryotes, including human CDC14A, CDC14B, and CDC14C. These enzymes are critical for reversing cyclin-dependent kinase (CDK)-mediated phosphorylation events, ensuring proper mitotic exit, cytokinesis, and genome stability.
Structurally, Cdc14 proteins contain an N-terminal catalytic domain with a conserved phosphatase motif and a C-terminal regulatory region that influences substrate binding and subcellular localization. In humans, CDC14A and CDC14B are implicated in centrosome maturation, spindle assembly, and DNA repair, while their dysregulation is linked to cancers and neurodegenerative disorders.
Recombinant CDC14 proteins are engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to study their biochemical properties, interaction networks, and therapeutic potential. These proteins are typically purified via affinity tags (e.g., His-tag) and validated for phosphatase activity using fluorogenic substrates or phosphopeptides. Recombinant CDC14 serves as a tool to dissect its role in cell cycle checkpoints, mitotic fidelity, and ciliogenesis, as well as to screen inhibitors for cancer therapy.
Recent studies highlight CDC14's context-dependent functions in tumor suppression or oncogenesis, urging further exploration of isoform-specific mechanisms. The availability of recombinant CDC14 proteins accelerates functional studies, aiding the development of targeted therapies for cell cycle-related diseases.
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