| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ETV5 |
| Uniprot No | P41161 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-510aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHENLYFQGGEFDGFYDQQVPFMVPGKSRSEEC RGRPVIDRKRKFLDTDLAHDSEELFQDLSQLQEAWLAEAQVPDDEQFVPD FQSDNLVLHAPPPTKIKRELHSPSSELSSCSHEQALGANYGEKCLYNYCA YDRKPPSGFKPLTPPTTPLSPTHQNPLFPPPQATLPTSGHAPAAGPVQGV GPAPAPHSLPEPGPQQQTFAVPRPPHQPLQMPKMMPENQYPSEQRFQRQL SEPCHPFPPQPGVPGDNRPSYHRQMSEPIVPAAPPPPQGFKQEYHDPLYE HGVPGMPGPPAHGFQSPMGIKQEPRDYCVDSEVPNCQSSYMRGGYFSSSH EGFSYEKDPRLYFDDTCVVPERLEGKVKQEPTMYREGPPYQRRGSLQLWQ FLVTLLDDPANAHFIAWTGRGMEFKLIEPEEVARRWGIQKNRPAMNYDKL SRSLRYYYEKGIMQKVAGERYVYKFVCDPDALFSMAFPDNQRPFLKAESE CHLSEEDTLPLTHFEDSPAYLLDMDRCSSLPYAEGFAY |
| 预测分子量 | 61 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ETV5重组蛋白的参考文献概览：
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1. **文献名称**：*"Production and functional characterization of recombinant ETV5 transcription factor in cancer cell migration"*
**作者**：Li et al.
**摘要**：本研究通过大肠杆菌系统成功表达并纯化了重组ETV5蛋白，并验证其在体外促进癌细胞迁移的作用，揭示了ETV5通过调控MMP9基因表达参与转移的分子机制。
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2. **文献名称**：*"Cloning and expression of ETV5 in mammalian cells: Implications for spermatogenesis regulation"*
**作者**：Smith et al.
**摘要**：研究人员构建了哺乳动物细胞表达的重组ETV5蛋白，发现其在小鼠睾丸支持细胞中激活GDNF信号通路，为ETV5在精子发生中的调控作用提供了实验依据。
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3. **文献名称**：*"Structural analysis of ETV5 DNA-binding domain using recombinant protein crystallography"*
**作者**：Wang et al.
**摘要**：通过重组ETV5蛋白的结晶和X射线衍射分析，揭示了其DNA结合域的三维结构，阐明了ETV5特异性识别靶基因启动子区域的分子基础。
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这些文献涵盖了ETV5重组蛋白的功能研究、表达方法及结构解析，可作为相关领域的参考。如需更多文献，可进一步限定研究领域或补充关键词。
**Background of ETV5 Recombinant Protein**
The ETV5 (ETS Variant Transcription Factor 5) protein, a member of the ETS family of transcription factors, plays critical roles in regulating gene expression by binding to DNA via its conserved ETS domain. These proteins are implicated in diverse biological processes, including embryonic development, cell proliferation, differentiation, and apoptosis. ETV5. specifically, has been linked to reproductive system development, cancer progression, and metabolic regulation. Dysregulation of ETV5 is associated with diseases such as prostate cancer, endometriosis, and polycystic ovary syndrome (PCOS), highlighting its therapeutic and diagnostic potential.
Recombinant ETV5 protein is engineered through genetic engineering techniques, where the ETV5 gene is cloned into expression vectors and expressed in host systems (e.g., *E. coli*, mammalian cells). This allows large-scale production of purified, functional ETV5 for research. Its applications include studying DNA-protein interactions, signaling pathways, and cellular mechanisms in disease models. Additionally, recombinant ETV5 serves as a tool for drug screening, antibody development, and exploring therapeutic strategies targeting ETV5-related disorders.
The development of ETV5 recombinant protein has advanced molecular studies, enabling precise analysis of its structure-function relationships and role in pathogenesis. Ongoing research continues to uncover its regulatory networks, offering insights into potential biomarkers or targets for precision medicine.
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