| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHb6 |
| Uniprot No | Q9Y5E3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-794aa |
| 氨基酸序列 | MMQTKVQNKKRQVAFFILLMLWGEVGSESIQYSVLEETESGTFVANLTKDLGLRVGELASRGARVVFKGNRQHLQFDPQTHDLLLNEKLDREELCGSTEPCVLPFQVLLENPLQFFQASLRVRDINDHAPEFPAREMLLKISEITMPGKIFPLKMAHDLDTGSNGLQRYTISSNPHFHVLTRNRSEGRKFPELVLDKPLDREEQPQLRLTLIALDGGSPPRSGTSEIQIQVLDINDNVPEFAQELYEAQVPENNPLGSLVITVSARDLDAGSFGKVSYALFQVDDVNQPFEINAITGEIRLRKALDFEEIQSYDVDVEATDGGGLSGKCSLVVRVLDVNDNAPELTMSFFISLIPENLPEITVAVFSVSDADSGHNQQVICSIENNLPFLLRPSVENFYTLVTEGALDRESRAEYNITITVTDLGTPRLKTQQSITVQVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGINAQVTYSLLPPQDPHLPLSSLVSINADNGHLFALRSLDYEALQSFEFRVGATDRGSPALSSEALVRLLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATELGLFGVWAHNGEVRTARLLSERDAAKHRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADSLTVYLVVALASVSSLFLFSVLLFVAVRLCRRSRAASVGRYSVPEGPFPGHLVDVSGTGTLSQSYQYKVCLTGGSETNEFKFLKPIMPNFPPQGTEREMEETPTSRNSFPFS |
| 预测分子量 | 87,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHb6重组蛋白的3篇参考文献示例(注：部分信息基于假设性内容整理，实际文献需通过学术数据库验证)：
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1. **标题**: *Expression and Functional Characterization of Recombinant Protocadherin-β6 in Neuronal Cells*
**作者**: Smith A, et al.
**摘要**: 本研究报道了人源PCDHb6重组蛋白在大肠杆菌中的高效表达与纯化，并通过体外细胞黏附实验证实其促进神经元突触形成的功能，为研究原钙粘蛋白在神经发育中的作用提供了工具。
2. **标题**: *Structural Insights into PCDHb6 Homophilic Binding via Recombinant Protein Crystallography*
**作者**: Zhang L, et al.
**摘要**: 通过重组PCDHb6胞外域的结晶和结构解析，揭示了其独特的同源二聚化模式，为理解原钙粘蛋白家族在细胞间识别中的分子机制提供了结构基础。
3. **标题**: *PCDHb6 Recombinant Protein Suppresses Tumor Migration in Breast Cancer Models*
**作者**: Kim H, et al.
**摘要**: 研究发现，外源性添加PCDHb6重组蛋白可抑制乳腺癌细胞的迁移和侵袭能力，提示其在肿瘤转移调控中的潜在治疗价值。
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**提示**：以上为模拟参考文献，实际文献需通过 **PubMed** (https://pubmed.ncbi.nlm.nih.gov) 或 **Google Scholar** 搜索关键词 "PCDHb6 recombinant protein" 获取。若研究较新或较冷门，建议扩展检索至原钙粘蛋白(protocadherin)家族相关重组蛋白研究。
PCDHb6 (Protocadherin Beta-6) is a member of the protocadherin beta gene cluster, a subgroup within the cadherin superfamily. Protocadherins are transmembrane proteins involved in cell-cell adhesion, neural circuit formation, and synaptic specificity, playing critical roles in neuronal development and tissue morphogenesis. The PCDH beta cluster, located on human chromosome 5. comprises 16 genes (PCDHb1-PCDHb16) encoding calcium-dependent adhesion proteins with diverse extracellular cadherin repeats and unique cytoplasmic domains. PCDHb6. specifically, is characterized by six extracellular cadherin domains, a transmembrane region, and a cytoplasmic tail that may interact with intracellular signaling molecules.
Recombinant PCDHb6 protein is engineered for functional studies to elucidate its role in neurodevelopment, cancer, or other diseases. Dysregulation of PCDHb6 has been linked to neurodevelopmental disorders and malignancies, where it may act as a tumor suppressor or promoter depending on context. For example, epigenetic silencing of PCDHb6 has been observed in colorectal and lung cancers, correlating with poor prognosis. Recombinant forms, typically produced in mammalian or insect cell systems to ensure proper post-translational modifications, enable in vitro analysis of its adhesive properties, signaling pathways, and interactions with binding partners like cytoskeletal proteins or other cadherins.
Research on recombinant PCDHb6 also aids in developing diagnostic tools or therapeutic strategies targeting cadherin-mediated processes. Its structure-function relationships, including calcium-binding motifs and homophilic binding specificity, are key focuses. Current studies leverage techniques like X-ray crystallography, cell adhesion assays, and CRISPR-mediated knockout models to dissect its biological significance, offering insights into neural connectivity and cancer metastasis mechanisms.
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