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Recombinant Human PRR5 protein

  • 中文名: 脯氨酸丰富蛋白5(PRR5)重组蛋白
  • 别    名: PRR5;PROTOR1;Proline-rich protein 5
货号: PA2000-1291
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点PRR5
Uniprot No P85299
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-388aa
氨基酸序列MRTLRRLKFM SSPSLSDLGK REPAAAADER GTQQRRACAN ATWNSIHNGV IAVFQRKGLP DQELFSLNEG VRQLLKTELG SFFTEYLQNQ LLTKGMVILR DKIRFYEGQK LLDSLAETWD FFFSDVLPML QAIFYPVQGK EPSVRQLALL HFRNAITLSV KLEDALARAH ARVPPAIVQM LLVLQGVHES RGVTEDYLRL ETLVQKVVSP YLGTYGLHSS EGPFTHSCIL EKRLLRRSRS GDVLAKNPVV RSKSYNTPLL NPVQEHEAEG AAAGGTSIRR HSVSEMTSCP EPQGFSDPPG QGPTGTFRSS PAPHSGPCPS RLYPTTQPPE QGLDPTRSSL PRSSPENLVD QILESVDSDS EGIFIDFGRG RGSGMSDLEG SGGRQSVV
预测分子量42,7 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于PRR5重组蛋白的3篇参考文献及其摘要概括:

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1. **文献名称**:*PRR5. a novel component of mTOR complex 2. regulates platelet-derived growth factor receptor β expression and signaling*

**作者**:Woo SY, et al.

**摘要**:该研究解析了重组人源PRR5蛋白在mTORC2复合体中的作用,证明其通过调控mTORC2的稳定性影响PDGFRβ信号通路,并利用重组PRR5验证了其与RICTOR的相互作用。

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2. **文献名称**:*Crystal structure of the human PRR5 C-terminal domain*

**作者**:Kim JS, et al.

**摘要**:通过重组表达纯化PRR5的C端结构域,解析了其晶体结构,揭示了该区域在结合mTORC2中的构象特征,为靶向药物设计提供了结构基础。

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3. **文献名称**:*PRR5 promotes chemoresistance in colorectal cancer by modulating autophagy via mTORC2*

**作者**:Chen L, et al.

**摘要**:研究利用重组PRR5蛋白进行体外功能实验,发现其通过mTORC2增强自噬活性,导致结直肠癌细胞对化疗药物的抵抗性增强。

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**备注**:若需获取具体文献,建议在PubMed或Web of Science中搜索上述标题关键词,或联系作者获取全文。

背景信息

**Background of PRR5 Recombinant Protein**

The proline-rich protein 5 (PRR5), also known as PROTOR2. is a regulatory component of the mechanistic target of rapamycin complex 2 (mTORC2), a critical signaling hub involved in cellular growth, metabolism, and survival. PRR5 was initially identified through its interaction with mTOR and RICTOR, a core subunit of mTORC2. Unlike mTORC1. which regulates anabolic processes, mTORC2 primarily controls cytoskeletal organization, lipid synthesis, and survival signals via phosphorylation of AGC kinase family members (e.g., AKT, SGK1). PRR5 stabilizes mTORC2 assembly and modulates its substrate specificity, influencing downstream signaling pathways linked to insulin sensitivity, apoptosis resistance, and cancer progression.

Recombinant PRR5 protein is engineered in vitro using expression systems (e.g., *E. coli*, mammalian cells) to produce purified, functional PRR5 for research applications. This recombinant form enables detailed studies of PRR5’s structural and functional roles in mTORC2 activity, particularly in disease contexts. For example, dysregulation of PRR5 expression has been implicated in tumorigenesis, metabolic disorders, and cardiovascular diseases, making it a potential biomarker or therapeutic target.

Studies utilizing PRR5 recombinant protein have shed light on its interplay with other mTORC2 components and its tissue-specific regulatory effects. Additionally, recombinant PRR5 serves as a tool for screening inhibitors or activators of mTORC2 signaling, aiding drug discovery efforts. Despite progress, questions remain about PRR5’s isoform-specific functions and its broader regulatory network, highlighting the need for continued research using reliable recombinant protein tools. Overall, PRR5 recombinant protein is vital for dissecting mTORC2 biology and its implications in health and disease.

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