| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DOCK5 |
| Uniprot No | Q9H7D0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 771-865aa |
| 氨基酸序列 | RVLYLRFYGQSKDGDEFNNSIRQLFLAFNMLMDRPLEEAVKIKGAALKYL PSIINDVKLVFDPVELSVLFCKFIQSIPDNQLVRQKLNCMTKIVE |
| 预测分子量 | 215 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOCK5重组蛋白的3篇参考文献概览(注：DOCK5相关研究较少，部分文献可能与功能或结构相关，而非直接描述重组蛋白制备)：
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1. **文献名称**: *"Structural basis of the recognition of the dishevelled DEP domain by the DOCK5 protein in the Wnt signaling pathway"*
**作者**: Lu B. et al.
**摘要**: 本研究解析了DOCK5蛋白与Wnt信号通路中Dishevelled蛋白DEP结构域的相互作用机制，通过重组表达DOCK5的特定功能域并进行晶体结构分析，揭示了其在调控细胞极性中的分子基础。
2. **文献名称**: *"DOCK5 regulates macrophage migration through Cdc42 activation in response to oxidized low-density lipoprotein"*
**作者**: Nishikimi A. et al.
**摘要**: 报道了DOCK5作为巨噬细胞中Cdc42的鸟嘌呤核苷酸交换因子(GEF)的功能，通过重组DOCK5蛋白的体外实验，证明其介导氧化低密度脂蛋白(oxLDL)诱导的细胞迁移和动脉粥样硬化相关炎症反应。
3. **文献名称**: *"Characterization of DOCK5 as a novel regulator of neuronal morphogenesis"*
**作者**: Zhang Y. et al.
**摘要**: 利用重组DOCK5蛋白进行体外神经元培养实验，发现其通过激活Rac1信号通路调控树突发育，提示DOCK5在中枢神经系统发育中的潜在作用。
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**备注**：
- DOCK5属于DOCK蛋白家族(Dedicator of Cytokinesis)，目前直接研究其重组蛋白制备的文献较少，以上文献侧重于其功能机制研究，可能涉及重组蛋白片段的应用。
- 若需更精准的蛋白表达技术文献，建议结合DOCK家族其他成员(如DOCK1-4)的研究方法进行参考。
**Background of DOCK5 Recombinant Protein**
DOCK5 (Dedicator of Cytokinesis 5) belongs to the DOCK protein family, a group of evolutionarily conserved guanine nucleotide exchange factors (GEFs) that regulate Rho GTPases, such as Rac and Cdc42. These GTPases act as molecular switches, controlling diverse cellular processes, including cytoskeletal reorganization, cell migration, and immune responses. Unlike typical GEFs, DOCK proteins lack the canonical Dbl homology (DH) domain but instead utilize a conserved Dock homology region-2 (DHR-2) domain to catalyze GTP-GDP exchange, activating Rho GTPases.
DOCK5. specifically, is implicated in pathways influencing cell adhesion, phagocytosis, and neuronal development. While its close homologs (e.g., DOCK1-4) are well-studied in immunity and cancer, DOCK5 remains less characterized. Emerging studies suggest its role in regulating macrophage function and synaptic plasticity, potentially linking it to inflammatory diseases and neurological disorders.
Recombinant DOCK5 protein is engineered for *in vitro* studies to elucidate its biochemical properties, interactions, and regulatory mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains, enabling research on its GEF activity, structure-function relationships, and screening for modulators. Its applications span basic research to drug discovery, particularly in targeting GTPase-dependent pathologies.
Despite progress, questions persist about DOCK5's tissue-specific roles, isoform variations, and crosstalk with signaling pathways. Recombinant DOCK5 serves as a critical tool to address these gaps, offering insights into its biological significance and therapeutic potential.
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