| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DNAJC15 |
| Uniprot No | Q9Y5T4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-150aa |
| 氨基酸序列 | MAARGVIAPVGESLRYAEYLQPSAKRPDADVDQQRLVRSLIAVGLGVAALAFAGRYAFRIWKPLEQVITETAKKISTPSFSSYYKGGFEQKMSRREAGLILGVSPSAGKAKIRTAHRRVMILNHPDKGGSPYVAAKINEAKDLLETTTKH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DNAJC15重组蛋白的3篇参考文献(示例为虚构，仅供参考)：
1. **文献名称**：*DNAJC15 Recombinant Protein Suppresses Tumor Growth via Mitochondrial Apoptosis Pathway*
**作者**：Zhang et al. (2020)
**摘要**：研究通过表达DNAJC15重组蛋白，发现其能激活线粒体依赖的凋亡通路，抑制乳腺癌细胞增殖，提示其潜在抗肿瘤应用。
2. **文献名称**：*Structural and Functional Analysis of DNAJC15 in Mitochondrial Membrane Stability*
**作者**：Chen et al. (2018)
**摘要**：利用重组DNAJC15蛋白进行体外实验，揭示其通过结合BAX蛋白调控线粒体膜通透性，影响细胞应激反应。
3. **文献名称**：*DNAJC15 Recombinant Protein Enhances Adipocyte Differentiation via AMPK Signaling*
**作者**：Lee et al. (2021)
**摘要**：通过重组蛋白功能研究，表明DNAJC15通过AMPK通路促进脂肪细胞分化，可能参与代谢疾病调控。
4. **文献名称**：*Purification and Characterization of Human DNAJC15 Recombinant Protein Expressed in E. coli*
**作者**：Smith et al. (2017)
**摘要**：报道DNAJC15重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证其分子伴侣活性。
(注：以上内容为模拟生成，实际文献需通过学术数据库查询。)
DNAJC15 is a member of the DNAJ/Hsp40 protein family, which functions as co-chaperones to regulate the ATPase activity of Hsp70 chaperones. It is localized primarily in mitochondria and plays a role in mitochondrial protein quality control, stress response, and apoptosis. The protein contains a conserved J-domain responsible for Hsp70 interaction and a transmembrane domain anchoring it to the mitochondrial inner membrane. Its C-terminal region includes a thioredoxin-like fold, suggesting potential redox regulatory functions.
Research highlights DNAJC15's involvement in mitochondrial autophagy (mitophagy) and its interaction with PINK1/Parkin pathways, linking it to neurodegenerative diseases like Parkinson's. Dysregulation of DNAJC15 has been observed in cancers, where it may act as either an oncogene or tumor suppressor depending on context. For instance, its downregulation in breast and liver cancers correlates with poor prognosis, while overexpression in certain models inhibits tumor growth.
Recombinant DNAJC15 protein is commonly produced in E. coli or mammalian expression systems for structural and functional studies. These studies focus on elucidating its role in mitochondrial dynamics, energy metabolism, and stress adaptation. The recombinant protein serves as a tool for investigating molecular mechanisms of diseases associated with mitochondrial dysfunction and for screening potential therapeutic compounds targeting chaperone networks. Recent studies also explore its potential as a biomarker for mitochondrial-related disorders.
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