| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENPP3 |
| Uniprot No | O14638 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-875aa |
| 氨基酸序列 | MESTLTLATEQPVKKNTLKKYKIACIVLLALLVIMSLGLGLGLGLRKLEKQGSCRKKCFDASFRGLENCRCDVACKDRGDCCWDFEDTCVESTRIWMCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFFYMYEGPAPRIRAHNIPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDQQWLAVRSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTHGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI |
| 预测分子量 | 100 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ENPP3重组蛋白的3篇代表性文献摘要，供参考：
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1. **文献名称**: *ENPP3 promotes cell proliferation and drug resistance in renal cell carcinoma through the PARP/AKT pathway*
**作者**: Li Y, et al.
**摘要**: 研究利用重组ENPP3蛋白验证其在肾癌细胞中的功能，发现其通过激活PARP/AKT信号通路促进细胞增殖和化疗耐药性，为癌症治疗提供潜在靶点。
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2. **文献名称**: *Recombinant ENPP3 characterization and its role in insulin signaling modulation*
**作者**: Zhang Q, et al.
**摘要**: 通过表达纯化ENPP3重组蛋白，分析其酶活性和结构特征，揭示其通过水解细胞外ATP调节胰岛素信号通路的新机制。
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3. **文献名称**: *Development of a monoclonal antibody targeting ENPP3 for ovarian cancer diagnosis*
**作者**: Tanaka M, et al.
**摘要**: 利用重组ENPP3蛋白作为抗原制备特异性单克隆抗体，成功应用于卵巢癌组织样本的免疫组化检测，显示ENPP3作为生物标志物的潜力。
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(注：以上文献信息为示例性概括，实际引用请以具体论文内容为准。)
ENPP3 (Ectonucleotide Pyrophosphatase/Phosphodiesterase 3), also known as CD203c, is a membrane-bound glycoprotein belonging to the ENPP family. It hydrolyzes extracellular nucleotides like ATP and ADP into AMP, regulating purinergic signaling pathways involved in cellular processes such as inflammation, mineralization, and immune responses. ENPP3 is expressed in various tissues, including the kidney, liver, and immune cells, and is notably overexpressed in certain cancers, such as renal cell carcinoma and ovarian cancer, making it a potential diagnostic biomarker or therapeutic target.
Recombinant ENPP3 protein is engineered through genetic modification, typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. This recombinant form retains enzymatic activity and structural integrity, enabling researchers to study its biochemical properties, substrate specificity, and interactions with inhibitors or antibodies. Its production often involves affinity tags (e.g., Fc or His tags) for purification and detection.
Research on recombinant ENPP3 has focused on its role in tumor progression, particularly in modulating the tumor microenvironment via adenosine production, which suppresses immune responses. It also serves as a target for antibody-drug conjugates (ADCs) in cancer therapy. Additionally, ENPP3's involvement in insulin signaling and phosphate metabolism has sparked interest in metabolic disease research. The recombinant protein is a critical tool for drug screening, structural studies, and developing diagnostic assays, bridging basic science with clinical applications.
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