Recombinant Human TCAM1 protein

中文名： 睾丸细胞粘附分子1(TCAM1)重组蛋白
别名： TCAM1；PRVTIRB；TRIF；TIR domain-containing adapter molecule 1

货号: PA2000-1286

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	TCAM1
Uniprot No	Q8IUC6
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-712aa
氨基酸序列	MACTGPSLPSAFDILGAAGQDKLLYLKHKLKTPRPGCQGQDLLHAMVLLKLGQETEARISLEALKADAVARLVARQWAGVDSTEDPEEPPDVSWAVARLYHLLAEEKLCPASLRDVAYQEAVRTLSSRDDHRLGELQDEARNRCGWDIAGDPGSIRTLQSNLGCLPPSSALPSGTRSLPRPIDGVSDWSQGCSLRSTGSPASLASNLEISQSPTMPFLSLHRSPHGPSKLCDDPQASLVPEPVPGGCQEPEEMSWPPSGEIASPPELPSSPPPGLPEVAPDATSTGLPDTPAAPETSTNYPVECTEGSAGPQSLPLPILEPVKNPCSVKDQTPLQLSVEDTTSPNTKPCPPTPTTPETSPPPPPPPPSSTPCSAHLTPSSLFPSSLESSSEQKFYNFVILHARADEHIALRVREKLEALGVPDGATFCEDFQVPGRGELSCLQDAIDHSAFIILLLTSNFDCRLSLHQVNQAMMSNLTRQGSPDCVIPFLPLESSPAQLSSDTASLLSGLVRLDEHSQIFARKVANTFKPHRLQARKAMWRKEQDTRALREQSQHLDGERMQAAALNAAYSAYLQSYLSYQAQMEQLQVAFGSHMSFGTGAPYGARMPFGGQVPLGAPPPFPTWPGCPQPPPLHAWQAGTPPPPSPQPAAFPQSLPFPQSPAFPTASPAPPQSPGLQPLIIHHAQMVQLGLNNHMWNQRGSQAPEDKTQEAE
预测分子量	76,4 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于TCAM1重组蛋白的参考文献示例(注：以下内容为模拟生成，实际文献可能需要根据具体研究领域核实)：

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1. **文献名称**：*Cloning and Functional Characterization of Recombinant TCAM1 in T-cell Adhesion*

**作者**：Zhang L., et al.

**摘要**：本研究成功克隆了人源TCAM1基因，并在HEK293细胞中表达了重组TCAM1蛋白。通过流式细胞术和免疫共沉淀实验，证实了该蛋白在T细胞黏附过程中的作用，为研究免疫调节机制提供了工具。

2. **文献名称**：*Structural Analysis of Recombinant TCAM1 via X-ray Crystallography*

**作者**：Smith J.R., Patel K.

**摘要**：利用大肠杆菌表达系统纯化TCAM1重组蛋白，并通过X射线晶体学解析其三维结构。研究发现TCAM1的特定结构域可能参与配体结合，为靶向药物设计奠定基础。

3. **文献名称**：*TCAM1 Recombinant Protein in Cancer Immunotherapy: Preclinical Evaluation*

**作者**：Wang Y., et al.

**摘要**：评估重组TCAM1蛋白在肿瘤模型中的免疫激活效应。实验表明，TCAM1能增强T细胞对癌细胞的识别，提示其在免疫治疗中的潜在应用价值。

4. **文献名称**：*Expression Optimization and Functional Screening of TCAM1 in Insect Cells*

**作者**：Gomez S., Müller T.

**摘要**：在杆状病毒-昆虫细胞系统中优化TCAM1重组蛋白表达条件，并通过体外功能实验验证其参与炎症信号通路的调控机制。

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如需真实文献，建议通过PubMed或Web of Science检索关键词“TCAM1 recombinant protein”或结合具体研究背景细化搜索。

背景信息

TCAM1 (Testis Cell Adhesion Molecule 1), also known as SPAG11 or HE2. is a protein encoded by the SPAG11 gene located on human chromosome 8. It belongs to the β-defensin protein family, which is characterized by antimicrobial properties and roles in innate immunity. TCAM1 is predominantly expressed in the male reproductive tract, particularly in the epididymis, where it contributes to sperm maturation, immune defense, and cell-cell adhesion processes. Its structural features include conserved cysteine residues forming disulfide bonds, critical for its functional stability.

Recombinant TCAM1 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to produce purified, biologically active forms for research. Its study has revealed dual functionality: as an antimicrobial agent against pathogens like E. coli and Chlamydia, and as a modulator of cellular signaling pathways. TCAM1 interacts with glycosaminoglycans and chemokine receptors, suggesting roles in immune cell recruitment and inflammation regulation.

In biomedical research, recombinant TCAM1 aids in exploring reproductive biology, host-pathogen interactions, and its potential as a therapeutic target for infertility or infections. Recent studies also investigate its aberrant expression in cancers, implicating it in tumor microenvironment modulation. Despite progress, mechanistic details of its adhesion-related functions and tissue-specific regulation remain under investigation. Its multifunctional nature positions TCAM1 as a compelling subject for both basic science and translational applications.