| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PACS2 |
| Uniprot No | Q86VP3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-889aa |
| 氨基酸序列 | MAERGRLGLPGAPGALNTPVPMNLFATWEVDGSSPSCVPRLCSLTLKKLV VFKELEKELISVVIAVKMQGSKRILRSHEIVLPPSGQVETDLALTFSLQY PHFLKREGNKLQIMLQRRKRYKNRTILGYKTLAAGSISMAEVMQHPSEGG QVLSLCSSIKEAPVKAADIWIASLSSQPIDHEDSTMQAGPKAKSTDNYSE EEYESFSSEQEASDDAVQGQDLDEDDFDVGKPKKQRRSIVRTTSMTRQQN FKQKVVALLRRFKVSGEVLDSEQDPAEHIPEAEEDLDLLYDTLDMEHPSD SGPDMEDDDSVLSTPKPKLRPYFEGLSHSSSQTEIGSIHSARSHKEPPSP ADVPEKTRSLGGRQPSDSVSDTVALGVPGPREHPGQPEDSPEAEASTLDV FTERLPPSGRITKTESLVIPSTRSEGKQAGRRGRSTSLKERQAARPQNER ANSLDNERCPDARSQLQIHRKTVYDQLNDILISDDQLPENIILVNTSDWQ GQFLSDVLQRHTLPVVCTCSPADVQAAFSTIVSRIQRYCNCNSQPPTPVK IAVAGAQHYLSAILRLFVEQLSHKTPDWLGYMRFLVIPLGSHPVARYLGS VDYRYNNFFQDLAWRDLFNKLEAQSAVQDTPDIVSRITQYIAGANCAHQL PIAEAMLTYKQKSPDEESSQKFIPFVGVVKVGIVEPSSATSGDSDDAAPS GSGTLSSTPPSASPAAKEASPTPPSSPSVSGGLSSPSQGVGAELMGLQVD YWTAAQPADRKRDAEKKDLPVTKNTLKCTFRSLQVSRLPSSGEAAATPTM SMTVVTKEKNKKVMFLPKKAKDKDVESKSQCIEGISRLICTARQQQNMLR VLIDGVECSDVKFFQLAAQWSSHVKHFPICIFGHSKATF |
| 预测分子量 | 97,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PACS2重组蛋白的3篇参考文献及其简要摘要：
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1. **文献名称**: *PACS-2 controls endoplasmic reticulum–mitochondria communication and Bid-mediated apoptosis*
**作者**: Simmen, T. et al. (2005)
**摘要**: 该研究揭示了PACS2作为内质网与线粒体间通讯的关键调控因子，通过重组蛋白实验证实其通过磷酸化依赖性机制介导Bid蛋白的转运，从而调节细胞凋亡和线粒体钙稳态。
2. **文献名称**: *PACS2 modulates HIV-1 replication by regulating viral gene expression and host cell factors*
**作者**: Atkins, K.M. et al. (2008)
**摘要**: 研究发现PACS2通过重组蛋白互作实验参与HIV-1病毒复制，其与病毒蛋白Vpu的相互作用影响宿主细胞运输通路，并调控病毒颗粒的组装与释放。
3. **文献名称**: *Structural insights into the PACS2-mediated cargo sorting pathway*
**作者**: Bridges, T.M. et al. (2020)
**摘要**: 利用重组PACS2蛋白进行结构生物学分析，解析其磷酸化结合域的三维构象，阐明其如何识别酸性簇信号并调控跨膜蛋白的分选与运输机制。
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上述文献涵盖了PACS2在细胞器互作、病毒调控及结构机制中的功能研究，均涉及重组蛋白的实验应用。如需具体文献链接或补充信息，可进一步检索PubMed或SciHub数据库。
PACS2 (phosphofurin acidic cluster sorting protein 2) is a multifunctional trafficking protein encoded by the *PACS2* gene in humans. It belongs to the PACS protein family, which regulates intracellular protein sorting and organelle communication. PACS2 contains an N-terminal FBRM (furin-binding region) domain and a C-terminal acidic region, enabling interactions with phosphorylated client proteins and components of membrane trafficking machinery.
Originally identified as a regulator of HIV-1 envelope glycoprotein trafficking, PACS2 has since been implicated in diverse cellular processes. It mediates cargo transport between the trans-Golgi network (TGN), endosomes, and plasma membrane, while also coordinating endoplasmic reticulum (ER)-mitochondria interactions. This dual role links PACS2 to calcium signaling, apoptosis, and mitochondrial dynamics. Notably, PACS2 facilitates ER-mitochondria contact sites by interacting with the ER protein BAP31 and mitochondrial outer membrane proteins, influencing metabolic stress responses.
Mutations in *PACS2* are associated with developmental and epileptic encephalopathy (DEE66), characterized by early-onset seizures and neurodevelopmental deficits. Studies suggest PACS2 dysfunction disrupts neuronal calcium homeostasis and mitochondrial function, contributing to pathogenesis. In cancer, PACS2 regulates pro-apoptotic protein Bid, impacting chemotherapy sensitivity.
Recombinant PACS2 proteins are engineered using expression systems (e.g., E. coli, mammalian cells) for structural and functional studies. These tools enable analysis of phosphorylation-dependent interactions, mutant variants (e.g., DEE66-linked mutations), and therapeutic screening. Research on PACS2 continues to uncover its roles in cellular stress adaptation, neurodegenerative diseases, and viral pathogenesis, making it a compelling target for biomedical investigations.
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