纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | IGSF12 |
Uniprot No | Q9UGN4 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-178aa |
氨基酸序列 | MWLPWALLLL WVPGCFALSK CRTVAGPVGG SLSVQCPYEK EHRTLNKYWC RPPQIFLCDK IVETKGSAGK RNGRVSIRDS PANLSFTVTL ENLTEEDAGT YWCGVDTPWL RDFHDPVVEV EVSVFPASTS MTPASITAAK TSTITTAFPP VSSTTLFAVG ATHSASIQEE TEEVVNSQ |
预测分子量 | 19 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IGSF12重组蛋白的3-4篇假设性参考文献的概括(基于领域内常见研究方向,供参考):
1. **文献名称**:**"Structural and functional characterization of recombinant IGSF12 protein in cell adhesion"**
**作者**:Kimura T, et al.
**摘要**:本研究成功在大肠杆菌系统中表达并纯化了IGSF12重组蛋白,通过X射线晶体学解析其胞外结构域的三维结构,发现其免疫球蛋白样结构域参与介导同源细胞间黏附。功能实验表明,IGSF12可能通过调控细胞间连接影响上皮屏障功能。
2. **文献名称**:**"IGSF12 interacts with claudin-1 to regulate tight junction assembly in epithelial cells"**
**作者**:Zhang L, et al.
**摘要**:利用重组人IGSF12蛋白进行免疫共沉淀和体外结合实验,发现其与紧密连接蛋白claudin-1直接相互作用。过表达IGSF12重组蛋白可增强细胞间紧密连接的形成,提示其在维持上皮细胞极性中的潜在作用。
3. **文献名称**:**"Recombinant IGSF12 promotes tumor angiogenesis via VEGF receptor signaling"**
**作者**:Wang Y, et al.
**摘要**:通过哺乳动物细胞表达系统制备功能性IGSF12重组蛋白,发现其能激活内皮细胞中VEGFR2信号通路,促进血管生成。动物模型显示,IGSF12重组蛋白处理可加速肿瘤血管新生,提示其作为癌症治疗靶点的可能性。
4. **文献名称**:**"Proteomic profiling identifies IGSF12 as a novel biomarker in hepatocellular carcinoma"**
**作者**:Gupta S, et al.
**摘要**:通过重组IGSF12蛋白制备抗体并进行组织芯片分析,发现IGSF12在肝癌组织中显著高表达,且与患者预后不良相关。体外实验证实,重组IGSF12可增强肝癌细胞迁移能力,可能通过整合素信号通路发挥作用。
**注**:以上文献为示例性内容,实际研究需通过PubMed/Google Scholar等平台检索真实文献(关键词:IGSF12 recombinant protein, IGSF12 function)。
IGSF12 (Immunoglobulin Superfamily Member 12) is a transmembrane protein belonging to the immunoglobulin superfamily, a diverse group of cell surface molecules involved in cell adhesion, signaling, and immune responses. It is characterized by extracellular immunoglobulin (Ig)-like domains, a single-pass transmembrane region, and a short cytoplasmic tail. While its precise biological functions remain under investigation, IGSF12 has been implicated in cell-cell interactions, tissue organization, and immune regulation. Studies suggest potential roles in endothelial cell behavior, angiogenesis, and cancer progression, though mechanistic details are still emerging.
Recombinant IGSF12 protein is typically produced using heterologous expression systems, such as mammalian cells (e.g., HEK293) or insect cells, to ensure proper post-translational modifications and structural integrity. The purified protein often includes the extracellular domain, which mediates ligand binding and intercellular communication. Researchers utilize recombinant IGSF12 to study its interactions with potential binding partners, map functional domains, and investigate its signaling pathways through in vitro assays. It also serves as an antigen for antibody development and a tool for exploring therapeutic targeting in diseases where IGSF12 is dysregulated, such as certain cancers or inflammatory conditions.
Current challenges in IGSF12 research include clarifying its endogenous ligands, tissue-specific expression patterns, and downstream effectors. The availability of recombinant protein has accelerated structural studies (e.g., crystallography) and functional screens, though the protein's low natural abundance and limited prior characterization necessitate careful experimental validation. Ongoing work aims to define its physiological relevance and translational potential in biomedicine.
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