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Recombinant Human KPNa6 protein

  • 中文名: 亲核素α6(KPNa6)重组蛋白
  • 别    名: KPNa6;IPOA7;Importin subunit alpha-7
货号: PA2000-1279
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点KPNa6
Uniprot No O60684
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-536aa
氨基酸序列METMASPGKD NYRMKSYKNN ALNPEEMRRR REEEGIQLRK QKREQQLFKR RNVELINEEA AMFDSLLMDS YVSSTTGESV ITREMVEMLF SDDSDLQLAT TQKFRKLLSK EPSPPIDEVI NTPRVVDRFV EFLKRNENCT LQFEAAWALT NIASGTSQQT KIVIEAGAVP IFIELLNSDF EDVQEQAVWA LGNIAGDSSV CRDYVLNCSI LNPLLTLLTK STRLTMTRNA VWALSNLCRG KNPPPEFAKV SPCLPVLSRL LFSSDSDLLA DACWALSYLS DGPNEKIQAV IDSGVCRRLV ELLMHNDYKV ASPALRAVGN IVTGDDIQTQ VILNCSALPC LLHLLSSPKE SIRKEACWTI SNITAGNRAQ IQAVIDANIF PVLIEILQKA EFRTRKEAAW AITNATSGGT PEQIRYLVSL GCIKPLCDLL TVMDSKIVQV ALNGLENILR LGEQEGKRSG SGVNPYCGLI EEAYGLDKIE FLQSHENQEI YQKAFDLIEH YFGVEDDDSS LAPQVDETQQ QFIFQQPEAP MEGFQL
预测分子量60 kDa
蛋白标签His tag N-Terminus
缓冲液PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于KPNA6(Karyopherin α6/Importin α6)重组蛋白的3篇代表性文献摘要:

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1. **文献名称**: *"Recombinant expression and functional characterization of human Importin α6 in nuclear import assays"*

**作者**: Smith J et al.

**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人源KPNA6蛋白,验证其与核定位信号(NLS)的结合能力,并证明其在体外核转运实验中可介导特定转录因子(如Oct4)的入核运输。

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2. **文献名称**: *"Structural insights into the selectivity of Importin α6 for distinct cargo proteins"*

**作者**: Tanaka R et al.

**摘要**: 通过X射线晶体学解析了重组KPNA6蛋白与不同NLS肽段的复合物结构,揭示了其底物选择性的分子机制,发现其结合口袋的柔性结构域对识别干细胞相关蛋白具有特异性。

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3. **文献名称**: *"KPNA6 knockdown impairs neuronal differentiation via dysregulation of global nuclear transport"*

**作者**: Chen L et al.

**摘要**: 利用重组KPNA6蛋白进行功能补偿实验,证明其在神经元分化过程中通过调控STAT3等信号分子的核转运维持分化进程,为神经发育疾病机制提供了新视角。

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**备注**:以上文献为虚拟示例,实际研究中建议通过PubMed或Web of Science以“KPNA6 recombinant protein”“Importin α6 function”等关键词检索真实文献。若需具体文章,可提供领域方向进一步筛选。

背景信息

**Background of KPNa6 Recombinant Protein**

KPNa6. also known as Karyopherin subunit alpha-6 or Importin alpha-6. is a member of the importin alpha family of nuclear transport proteins. These proteins play a critical role in nucleocytoplasmic transport by mediating the translocation of cargo proteins containing classical nuclear localization signals (NLS) into the nucleus. KPNa6 functions as an adaptor molecule, binding to NLS-bearing cargoes and forming a complex with Importin beta-1 (KPNB1), which facilitates passage through nuclear pore complexes.

The importin alpha family consists of multiple isoforms, each exhibiting tissue-specific expression and varying affinities for distinct NLS sequences. KPNa6 is particularly noted for its role in developmental processes, immune regulation, and cellular differentiation. Studies suggest its involvement in the nuclear import of transcription factors and signaling molecules critical for cell cycle progression, apoptosis, and stress responses. Dysregulation of KPNa6 has been linked to pathological conditions, including cancer and viral infections, where altered nuclear transport mechanisms contribute to disease progression.

Recombinant KPNa6 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, biologically active form for research. This tool enables the study of nuclear transport mechanisms, protein-protein interactions, and NLS recognition specificity. Its applications extend to drug discovery, particularly in targeting nuclear transport pathways disrupted in diseases. Structural and functional analyses of recombinant KPNa6 have provided insights into the molecular basis of cargo selectivity and regulatory post-translational modifications, enhancing understanding of cellular trafficking dynamics.

Overall, KPNa6 recombinant protein serves as a vital resource for unraveling the complexities of nuclear transport biology and its implications in health and disease.

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