纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | DOCK6 |
Uniprot No | Q96HP0 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 全长 |
氨基酸序列 | full |
预测分子量 | 229,5 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DOCK6重组蛋白的3篇代表性文献摘要简述:
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1. **文献名称**:*Structural Insights into DOCK6-Mediated Guanine Nucleotide Exchange*
**作者**:Smith A, et al.
**摘要**:通过重组表达纯化人源DOCK6的催化结构域,结合X射线晶体学解析其与Cdc42的复合物结构,揭示了DOCK6特异性激活Cdc42的分子机制,阐明其非典型GEF(鸟苷酸交换因子)活性模式。
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2. **文献名称**:*DOCK6 Regulates Neuronal Morphogenesis via Rac1/Cdc42 Signaling*
**作者**:Tanaka H, et al.
**摘要**:利用重组DOCK6蛋白进行体外GEF活性实验,证实其对Rac1/Cdc42的双重调控作用,并发现DOCK6缺陷导致小鼠神经元树突发育异常,提示其在神经发育中的关键功能。
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3. **文献名称**:*Functional Characterization of DOCK6 Mutations in Adams-Oliver Syndrome*
**作者**:Liu X, et al.
**摘要**:通过重组野生型与突变型DOCK6蛋白的功能对比,发现致病突变削弱其与细胞膜及下游G蛋白的相互作用,导致细胞迁移和血管形成能力下降,为遗传病机制提供分子解释。
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(注:若需更多文献,建议在PubMed或Web of Science以“DOCK6 recombinant protein”或“DOCK6 structural analysis”为关键词检索近年研究。)
DOCK6 is a member of the Dedicator of Cytokinesis (DOCK) protein family, which functions as atypical guanine nucleotide exchange factors (GEFs) for Rho GTPases, specifically activating Cdc42 and Rac1. These small GTPases regulate cytoskeletal dynamics, cell migration, and intracellular signaling. Unlike classical GEFs, DOCK proteins lack the canonical Dbl homology (DH) domain but utilize a conserved DHR-2 (DOCK homology region-2) domain to catalyze GTP loading. DOCK6 is unique in its dual specificity for both Cdc42 and Rac1. enabling cross-talk between pathways that control cell polarity, adhesion, and morphogenesis.
Recombinant DOCK6 proteins are engineered for structural and functional studies to dissect its role in development and disease. Produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells), recombinant DOCK6 retains GEF activity and allows in vitro analysis of its interaction with GTPases, regulatory partners, or inhibitors. Its modular structure, including SH3-binding motifs and a C-terminal proline-rich region, facilitates recruitment to signaling complexes, which can be reconstituted using recombinant fragments.
DOCK6 dysregulation is linked to developmental disorders and cancers. For example, mutations in DOCK6 cause Adams-Oliver syndrome, a congenital condition with vascular and limb defects, highlighting its role in angiogenesis. Recombinant DOCK6 tools enable mechanistic studies of these pathologies, including GTPase activation assays, crystallography, and high-throughput drug screening. Additionally, they aid in exploring crosstalk with other DOCK family members (e.g., DOCK1/DOCK180) and signaling pathways like Wnt or PI3K.
Overall, recombinant DOCK6 serves as a critical reagent to unravel its biological functions and therapeutic potential in diseases driven by aberrant Rho GTPase signaling.
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