| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CADM2 |
| Uniprot No | Q8N3J6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-435aa |
| 氨基酸序列 | MIWKRSAVLRFYSVCGLLLQGSQGQFPLTQNVTVVEGGTAILTCRVDQNDNTSLQWSNPAQQTLYFDDKKALRDNRIELVRASWHELSISVSDVSLSDEGQYTCSLFTMPVKTSKAYLTVLGVPEKPQISGFSSPVMEGDLMQLTCKTSGSKPAADIRWFKNDKEIKDVKYLKEEDANRKTFTVSSTLDFRVDRSDDGVAVICRVDHESLNATPQVAMQVLEIHYTPSVKIIPSTPFPQEGQPLILTCESKGKPLPEPVLWTKDGGELPDPDRMVVSGRELNILFLNKTDNGTYRCEATNTIGQSSAEYVLIVHDVPNTLLPTTIIPSLTTATVTTTVAITTSPTTSATTSSIRDPNALAGQNGPDHALIGGIVAVVVFVTLCSIFLLGRYLARHKGTYLTNEAKGAEDAPDADTAIINAEGSQVNAEEKKEYFI |
| 预测分子量 | 47,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CADM2重组蛋白的参考文献示例(部分信息基于真实研究领域，但文献为虚构示例，仅供参考)：
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1. **文献名称**: *Structural characterization of CADM2 extracellular domain reveals homophilic adhesion mechanism*
**作者**: Li, X., Zhang, Y., & Wang, H.
**摘要**: 本研究通过重组表达CADM2的胞外结构域蛋白，利用X射线晶体学解析其三维结构，发现其通过免疫球蛋白样结构域介导同源粘附作用，揭示了CADM2在神经元间相互作用中的分子基础。
2. **文献名称**: *CADM2 recombinant protein modulates synaptic plasticity in hippocampal neurons*
**作者**: Chen, L., Tanaka, K., & Park, S.
**摘要**: 通过体外培养海马神经元并添加纯化的CADM2重组蛋白，研究发现其显著促进突触形成和树突棘密度增加，表明CADM2在突触可塑性中起关键调控作用。
3. **文献名称**: *Association of CADM2 variants with autism spectrum disorder and functional validation using recombinant protein assays*
**作者**: Smith, J., Doe, R., & Garcia, M.
**摘要**: 该研究结合遗传学分析与体外实验，发现自闭症患者中CADM2基因突变导致其重组蛋白的细胞粘附功能异常，提示CADM2功能障碍可能与神经发育疾病相关。
4. **文献名称**: *Development of a CADM2 recombinant protein-based ELISA for biomarker detection in neurodegenerative diseases*
**作者**: Kim, T., Lee, J., & Müller, U.
**摘要**: 研究团队开发了一种基于CADM2重组蛋白的ELISA检测方法，用于定量分析脑脊液中的CADM2水平，为阿尔茨海默病等神经退行性疾病的生物标志物研究提供新工具。
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**注意**：以上文献为示例性质，实际研究中需查询PubMed、Google Scholar等数据库获取真实引用。若需具体文献，建议使用关键词“CADM2 recombinant protein”或“CADM2 structure/function”进一步检索。
CADM2 (Cell Adhesion Molecule 2), also known as SynCAM2 or IGSF4D, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It plays critical roles in cell-cell adhesion, synaptic organization, and neural network formation. Structurally, CADM2 contains three immunoglobulin-like domains and a fibronectin type III repeat, enabling homophilic and heterophilic interactions with partners like CADM1 and CADM3.
Primarily expressed in the central nervous system, CADM2 is enriched at synaptic junctions, where it facilitates neuron-glia communication and regulates synaptic plasticity. Recent studies link CADM2 polymorphisms to neuropsychiatric conditions, including autism spectrum disorders, addiction-related behaviors, and major depressive disorder. Its extracellular domain mediates cell adhesion, while the intracellular PDZ-binding motif interacts with scaffolding proteins to influence signal transduction.
Recombinant CADM2 proteins are engineered for functional studies, typically produced in mammalian or insect cell systems to ensure proper post-translational modifications. These purified proteins enable in vitro analysis of adhesion mechanisms, receptor-ligand interactions, and drug discovery targeting neurological pathways. Notably, altered CADM2 expression has been observed in cancers, suggesting tumor-suppressive roles by inhibiting metastasis through cell adhesion maintenance.
As a research tool, recombinant CADM2 aids in deciphering neural circuit assembly and synaptic pathology, while its therapeutic potential in modulating synaptic connectivity remains under active investigation. Emerging evidence also implicates CADM2 in metabolic regulation, expanding its relevance beyond neurological contexts.
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