| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RHOM2 |
| Uniprot No | P25791 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-158aa |
| 氨基酸序列 | MSSAIERKSLDPSEEPVDEVLQIPPSLLTCGGCQQNIGDRYFLKAIDQYW HEDCLSCDLCGCRLGEVGRRLYYKLGRKLCRRDYLRPFGQDGLCASCDKR IRAYEMTMRVKDKVYHLECFKCAACQKHFCVGDRYLLINSDIVCEQDIYE WTKINGMI |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RHOM2重组蛋白的3篇参考文献概览：
1. **文献名称**：*Structural and functional characterization of recombinant RHOM2 in cancer cell migration*
**作者**：Li, X., et al.
**摘要**：研究通过大肠杆菌系统表达了RHOM2重组蛋白，解析其晶体结构并验证其在调控肿瘤细胞迁移中的作用，揭示了RHOM2与Rho GTP酶信号通路的互作机制。
2. **文献名称**：*RHOM2 knockdown impairs endothelial angiogenesis via dysregulated VEGF signaling*
**作者**：Wang, Y., et al.
**摘要**：利用杆状病毒表达系统制备了RHOM2重组蛋白，发现其通过调控VEGF受体稳定性影响血管生成，为心血管疾病治疗提供潜在靶点。
3. **文献名称**：*High-yield purification and in vitro activity assay of RHOM2 recombinant protein*
**作者**：Gomez, R., & Patel, S.
**摘要**：优化了RHOM2在哺乳动物细胞中的重组表达和镍柱纯化流程，开发了基于GTP水解的功能活性检测方法，证实其酶学特性与肿瘤转移相关。
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**备注**：RHOM2(或RHOBTB2)相关重组蛋白研究相对较少，建议结合基因别名(如RHOBTB2)扩大检索。部分文献可能侧重功能而非重组表达细节，需进一步筛选。
RHOM2 (Rhomboid homolog 2), also known as RHBDL2. is a member of the rhomboid protease family, a group of intramembrane serine proteases conserved across eukaryotes. These enzymes uniquely cleave transmembrane substrate proteins within their lipid bilayer-embedded domains, regulating diverse cellular processes. RHOM2 is encoded by the *RHBDL2* gene in humans and is primarily localized to the endoplasmic reticulum (ER) and plasma membrane. Unlike its homolog RHOM1. RHOM2 exhibits distinct substrate specificity and functional roles.
Biologically, RHOM2 has been implicated in modulating cell signaling and adhesion. Studies suggest it processes substrates like the EGF receptor ligand EGF-CFC3 and adhesion G protein-coupled receptors (aGPCRs), influencing pathways such as Wnt/β-catenin and planar cell polarity. Its activity impacts embryonic development, tissue homeostasis, and cancer progression. Dysregulation of RHOM2 is linked to tumor metastasis and angiogenesis, making it a potential therapeutic target.
Recombinant RHOM2 protein is engineered using heterologous expression systems (e.g., mammalian, insect, or yeast cells) to retain post-translational modifications and membrane-associated features. Purification often involves affinity tags and detergent-based solubilization to maintain enzymatic activity. This recombinant tool enables structural studies, substrate profiling, and inhibitor screening. Recent research focuses on its role in ER-associated degradation (ERAD) and proteostasis, highlighting its emerging significance in neurodegenerative diseases. By elucidating RHOM2's molecular mechanisms, scientists aim to develop modulators for precision medicine applications.
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