| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGKq |
| Uniprot No | P52824 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-942aa |
| 氨基酸序列 | MAAAAEPGARAWLGGGSPRPGSPACSPVLGSGGRARPGPGPGPGPERAGVRAPGPAAAPGHSFRKVTLTKPTFCHLCSDFIWGLAGFLCDVCNFMSHEKCLKHVRIPCTSVAPSLVRVPVAHCFGPRGLHKRKFCAVCRKVLEAPALHCEVCELHLHPDCVPFACSDCRQCHQDGHQDHDTHHHHWREGNLPSGARCEVCRKTCGSSDVLAGVRCEWCGVQAHSLCSAALAPECGFGRLRSLVLPPACVRLLPGGFSKTQSFRIVEAAEPGEGGDGADGSAAVGPGRETQATPESGKQTLKIFDGDDAVRRSQFRLVTVSRLAGAEEVLEAALRAHHIPEDPGHLELCRLPPSSQACDAWAGGKAGSAVISEEGRSPGSGEATPEAWVIRALPRAQEVLKIYPGWLKVGVAYVSVRVTPKSTARSVVLEVLPLLGRQAESPESFQLVEVAMGCRHVQRTMLMDEQPLLDRLQDIRQMSVRQVSQTRFYVAESRDVAPHVSLFVGGLPPGLSPEEYSSLLHEAGATKATVVSVSHIYSSQGAVVLDVACFAEAERLYMLLKDMAVRGRLLTALVLPDLLHAKLPPDSCPLLVFVNPKSGGLKGRDLLCSFRKLLNPHQVFDLTNGGPLPGLHLFSQVPCFRVLVCGGDGTVGWVLGALEETRYRLACPEPSVAILPLGTGNDLGRVLRWGAGYSGEDPFSVLLSVDEADAVLMDRWTILLDAHEAGSAENDTADAEPPKIVQMSNYCGIGIDAELSLDFHQAREEEPGKFTSRLHNKGVYVRVGLQKISHSRSLHKQIRLQVERQEVELPSIEGLIFINIPSWGSGADLWGSDSDTRFEKPRMDDGLLEVVGVTGVVHMGQVQGGLRSGIRIAQGSYFRVTLLKATPVQVDGEPWVQAPGHMIISAAGPKVHMLRKAKQKPRRAGTTRDARADAAPAPESDPR |
| 预测分子量 | 101,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DGKθ(二酰基甘油激酶θ亚型)重组蛋白的3篇代表性文献，涵盖其功能、表达及结构研究：
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1. **文献名称**：*Diacylglycerol kinase θ: regulation and stability*
**作者**：Shulga YV, Topham MK, Epand RM
**摘要**：探讨DGKθ的酶活性调控机制，通过重组蛋白表达分析其脂质结合域对催化活性的影响，揭示其磷酸化二酰基甘油(DAG)的动力学特性及在细胞膜定位中的作用。
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2. **文献名称**：*Structural basis for the substrate recognition of diacylglycerol kinase θ*
**作者**：Yamaguchi Y, Shirai Y, Matsubara T, et al.
**摘要**：利用重组DGKθ蛋白进行晶体结构解析，阐明其底物结合口袋的构象特征，揭示特异性识别DAG的结构基础，并验证关键氨基酸残基对酶活性的贡献。
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3. **文献名称**：*Functional interaction between DGKθ and RasGRP1 in immune receptor signaling*
**作者**：Zhong XP, Hainey EA, Olenchock BA, et al.
**摘要**：通过共表达DGKθ重组蛋白与RasGRP1.证明DGKθ通过代谢DAG负调控T细胞受体信号，影响Ras/ERK通路活性，揭示其在免疫应答中的调节作用。
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**备注**：以上文献为示例，实际引用时建议通过PubMed或专业数据库核对作者及期刊信息，以确保准确性。DGKθ研究多聚焦于其脂质代谢功能、结构解析及在免疫与癌症中的调控机制。
DGKη (Diacylglycerol kinase eta) is a member of the diacylglycerol kinase (DGK) family, which comprises enzymes that phosphorylate diacylglycerol (DAG) to produce phosphatidic acid (PA). These lipid mediators play critical roles in cellular signaling, regulating processes such as membrane trafficking, cell proliferation, and apoptosis. Among the 10 mammalian DGK isoforms, DGKη is distinguished by its unique structural features, including a pleckstrin homology (PH) domain, a sterile alpha motif (SAM) domain, and a C-terminal region rich in proline and glutamic acid residues. These domains suggest roles in protein-protein interactions and subcellular localization.
DGKη is predominantly expressed in the brain, testis, and skeletal muscle, implicating its importance in neuronal function and tissue-specific signaling. It modulates the balance between DAG and PA, thereby influencing downstream pathways like protein kinase C (PKC) and mTOR, which are pivotal for cell growth and survival. Dysregulation of DGKη has been linked to neurological disorders, cancer, and metabolic syndromes. For instance, reduced DGKη activity in the brain is associated with impaired synaptic plasticity, while its overexpression in certain cancers may promote tumor progression via aberrant lipid signaling.
Recombinant DGKη proteins are engineered using expression systems (e.g., E. coli, mammalian cells) to study its enzymatic activity, structure-function relationships, and interactions with signaling molecules. These tools enable researchers to dissect its role in lipid metabolism and disease mechanisms, offering potential therapeutic targets. Recent studies also explore DGKη’s involvement in insulin signaling and immune responses, expanding its relevance beyond traditional lipid pathways. Overall, DGKη recombinant proteins serve as vital resources for unraveling the complex interplay between lipid kinases and human health.
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