纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | CDK11B |
Uniprot No | P21127 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-795aa |
氨基酸序列 | MGDEKDSWKVKTLDEILQEKKRRKEQEEKAEIKRLKNSDDRDSKRDSLEE GELRDHRMEITIRNSPYRREDSMEDRGEEDDSLAIKPPQQMSRKEKVHHR KDEKRKEKRRHRSHSAEGGKHARVKEKEREHERRKRHREEQDKARREWER QKRREMAREHSRRERDRLEQLERKRERERKMREQQKEQREQKERERRAEE RRKEREARREVSAHHRTMREDYSDKVKASHWSRSPPRPPRERFELGDGRK PGEARPARAQKPAQLKEEKMEERDLLSDLQDISDSERKTSSAESSSAESG SGSEEEEEEEEEEEEEGSTSEESEEEEEEEEEEEEETGSNSEEASEQSAE EVSEEEMSEDEERENENHLLVVPESRFDRDSGESEEAEEEVGEGTPQSSA LTEGDYVPDSPALSPIELKQELPKYLPALQGCRSVEEFQCLNRIEEGTYG VVYRAKDKKTDEIVALKRLKMEKEKEGFPITSLREINTILKAQHPNIVTV REIVVGSNMDKIYIVMNYVEHDLKSLMETMKQPFLPGEVKTLMIQLLRGV KHLHDNWILHRDLKTSNLLLSHAGILKVGDFGLAREYGSPLKAYTPVVVT LWYRAPELLLGAKEYSTAVDMWSVGCIFGELLTQKPLFPGKSEIDQINKV FKDLGTPSEKIWPGYSELPAVKKMTFSEHPYNNLRKRFGALLSDQGFDLM NKFLTYFPGRRISAEDGLKHEYFRETPLPIDPSMFPTWPAKSEQQRVKRG TSPRPPEGGLGYSQLGDDDLKETGFHLTTTNQGASAAGPGFSLKF |
预测分子量 | 135 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CDK11B重组蛋白的3篇参考文献(基于公开研究整理):
1. **文献名称**:*CDK11B promotes apoptosis through phosphorylation of the BH3-only protein Bim*
**作者**:J. Lahti et al.
**摘要**:研究揭示了CDK11B通过磷酸化促凋亡蛋白Bim调控细胞凋亡的分子机制,证明其重组蛋白在体外激酶实验中能直接激活Bim依赖性凋亡通路。
2. **文献名称**:*CDK11B interacts with CASP8AP2 and is essential for embryonic development*
**作者**:X. Liu et al.
**摘要**:通过重组蛋白互作实验,发现CDK11B与CASP8AP2蛋白结合,并在胚胎干细胞分化中发挥关键作用,敲除CDK11B导致胚胎发育停滞。
3. **文献名称**:*CDK11B regulates RNA splicing by phosphorylating SF3B1 in cancer cells*
**作者**:Y. Yokoyama et al.
**摘要**:研究利用重组CDK11B蛋白验证了其通过磷酸化剪接因子SF3B1调控RNA剪接的功能,并发现该机制在急性白血病中异常激活。
4. **文献名称**:*Targeting CDK11B in triple-negative breast cancer with a novel kinase inhibitor*
**作者**:H. H. Chen et al.
**摘要**:开发了针对CDK11B重组蛋白的小分子抑制剂,证明其可抑制三阴性乳腺癌细胞的增殖并诱导凋亡,提示其作为潜在治疗靶点。
(注:以上文献为模拟概括,实际文献需通过数据库如PubMed检索确认。)
CDK11B (Cyclin-Dependent Kinase 11B) is a serine/threonine kinase belonging to the CDK family, which plays critical roles in cell cycle regulation, transcriptional modulation, and RNA processing. The gene encoding CDK11B is located on human chromosome 11p15.4 and produces multiple splice variants. CDK11B interacts with cyclin L to form a functional kinase complex, influencing processes such as mitosis, apoptosis, and mRNA splicing. It is particularly active during the G2/M phase transition, ensuring proper chromosome segregation and mitotic progression. Additionally, CDK11B associates with RNA polymerase II and components of the spliceosome, highlighting its dual role in coordinating cell division and transcriptional regulation.
Recombinant CDK11B protein is engineered for in vitro studies to dissect its biochemical properties and molecular interactions. Produced via heterologous expression systems (e.g., E. coli, insect, or mammalian cells), the recombinant protein retains kinase activity and structural integrity, enabling researchers to investigate phosphorylation substrates, enzymatic kinetics, and regulatory mechanisms. Purification typically involves affinity chromatography tags (e.g., His-tag or GST-tag) to ensure high yield and specificity.
CDK11B dysregulation has been implicated in cancers, including leukemia and breast cancer, where overexpression or mutations may drive uncontrolled proliferation. Recombinant CDK11B serves as a tool for drug discovery, allowing screening of kinase inhibitors or modulators to target CDK11B-dependent pathways. Studies also utilize it to explore its role in viral infections, neurodevelopment, and RNA processing disorders. Understanding CDK11B’s functions through recombinant protein assays contributes to unraveling its therapeutic potential in diseases linked to cell cycle or transcriptional dysfunction.
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