| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OPN5 |
| Uniprot No | Q6U736 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-354aa |
| 氨基酸序列 | MALNHTALPQDERLPHYLRDGDPFASKLSWEADLVAGFYLTIIGILSTFGNGYVLYMSSRRKKKLRPAEIMTINLAVCDLGISVVGKPFTIISCFCHRWVFGWIGCRWYGWAGFFFGCGSLITMTAVSLDRYLKICYLSYGVWLKRKHAYICLAAIWAYASFWTTMPLVGLGDYVPEPFGTSCTLDWWLAQASVGGQVFILNILFFCLLLPTAVIVFSYVKIIAKVKSSSKEVAHFDSRIHSSHVLEMKLTKVAMLICAGFLIAWIPYAVVSVWSAFGRPDSIPIQLSVVPTLLAKSAAMYNPIIYQVIDYKFACCQTGGLKATKKKSLEGFRLHTVTTVRKSSAVLEIHEEWE |
| 预测分子量 | 39,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OPN5重组蛋白的参考文献示例(注：内容基于假设性研究，实际文献需通过学术数据库检索确认)：
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1. **文献名称**：*Heterologous Expression and Spectral Tuning of OPN5 (Neuropsin) in Mammalian Cells*
**作者**：Yamashita T, et al.
**摘要**：研究报道了人源OPN5在HEK293细胞中的重组表达，通过光谱分析发现其最大吸收波长在紫外光区域(~380 nm)，证实了OPN5作为紫外光敏感视蛋白的特性，并探讨了其在不同pH环境下的光响应稳定性。
2. **文献名称**：*Functional Characterization of Recombinant OPN5 in Circadian Photoentrainment*
**作者**：Kojima D, et al.
**摘要**：利用昆虫表达系统(Sf9细胞)制备重组OPN5蛋白，结合体外钙离子成像技术，证明OPN5通过Gq信号通路介导光诱导的细胞内钙信号，推测其在小鼠视网膜神经节细胞的昼夜节律调节中起关键作用。
3. **文献名称**：*Crystal Structure of Mouse OPN5 Reveals Unique Structural Features Among Vertebrate Opnins*
**作者**：Sato K, et al.
**摘要**：通过大肠杆菌表达系统获得小鼠OPN5重组蛋白的结晶结构，揭示了其视黄醛结合口袋的独特构象，解释了其对短波长光的敏感性，并对比了其他视蛋白家族成员的结构差异。
4. **文献名称**：*OPN5-Dependent Light Sensing in Cutaneous Thermoregulation*
**作者**：Buhr ED, et al.
**摘要**：研究利用重组OPN5蛋白进行体外光激活实验，结合基因敲除小鼠模型，发现皮肤中的OPN5通过感知环境紫外线调控体温适应性反应，揭示了非视觉光感受的新生理机制。
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**注意**：以上文献及摘要内容为示例性质，实际研究需参考具体发表的论文。建议通过PubMed或Google Scholar检索关键词“OPN5 recombinant”或“OPN5 expression”获取真实文献。
OPPN5 (Opsin 5), also known as neuropsin or G protein-coupled receptor 136 (GPR136), is a light-sensitive receptor belonging to the opsin family of G protein-coupled receptors (GPCRs). Initially identified in the mammalian brain and retina, OPN5 is evolutionarily conserved across vertebrates and functions as a UV-sensitive photopigment, with a peak absorption spectrum around 360 nm. Unlike classical visual opsins, OPN5 is primarily associated with non-image-forming photoreception, regulating circadian rhythms, seasonal reproduction, and light-dependent physiological processes in peripheral tissues. It is expressed in diverse locations, including the retina, skin, brain, and even reproductive organs, suggesting a broad role in light-mediated cellular signaling.
Recombinant OPN5 protein refers to the engineered form of this opsin produced in heterologous expression systems (e.g., mammalian cells, insect cells, or bacteria) for biochemical and functional studies. Its production enables researchers to study OPN5’s structure, ligand-binding properties, and downstream signaling pathways (e.g., Gi/o-mediated cAMP inhibition or MAP kinase activation) in controlled environments. Recombinant OPN5 is often tagged with fluorescent markers or purification handles (e.g., His-tag) to facilitate detection, localization, or protein-protein interaction assays. Applications include elucidating its role in UV light detection, circadian entrainment, and tissue-specific light responses. Recent studies also explore its potential in optogenetics or therapeutic targeting for disorders linked to light sensitivity or circadian dysregulation. Challenges remain in stabilizing the protein’s conformation and retaining photochemical activity in vitro, which are critical for functional assays.
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