| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTL4 |
| Uniprot No | Q53GD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-710aa |
| 氨基酸序列 | MGGKQRDEDDEAYGKPVKYDPSFRGPIKNRSCTDVICCVLFLLFILGYIVVGIVAWLYGDPRQVLYPRNSTGAYCGMGENKDKPYLLYFNIFSCILSSNIISVAENGLQCPTPQVCVSSCPEDPWTVGKNEFSQTVGEVFYTKNRNFCLPGVPWNMTVITSLQQELCPSFLLPSAPALGRCFPWTNVTPPALPGITNDTTIQQGISGLIDSLNARDISVKIFEDFAQSWYWILVALGVALVLSLLFILLLRLVAGPLVLVLILGVLGVLAYGIYYCWEEYRVLRDKGASISQLGFTTNLSAYQSVQETWLAALIVLAVLEAILLLMLIFLRQRIRIAIALLKEASKAVGQMMSTMFYPLVTFVLLLICIAYWAMTALYLATSGQPQYVLWASNISSPGCEKVPINTSCNPTAHLVNSSCPGLMCVFQGYSSKGLIQRSVFNLQIYGVLGLFWTLNWVLALGQCVLAGAFASFYWAFHKPQDIPTFPLISAFIRTLRYHTGSLAFGALILTLVQIARVILEYIDHKLRGVQNPVARCIMCCFKCCLWCLEKFIKFLNRNAYIMIAIYGKNFCVSAKNAFMLLMRNIVRVVVLDKVTDLLLFFGKLLVVGGVGVLSFFFFSGRIPGLGKDFKSPHLNYYWLPIMTSILGAYVIASGFFSVFGMCVDTLFLCFLEDLERNNGSLDRPYYMSKSLLKILGKKNEAPPDNKKRKK |
| 预测分子量 | 79,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与CTL4(推测为CTLA-4重组蛋白)相关的代表性文献示例(注：内容基于公开研究领域知识整理，建议通过学术数据库核对原文)：
1. **文献名称**： "Enhancement of Antitumor Immunity by CTLA-4 Blockade"
**作者**：Leach, D.R., et al. (1996)
**摘要**：首次证明通过抗体阻断CTLA-4可增强T细胞抗肿瘤反应，为癌症免疫治疗奠定理论基础，涉及重组CTLA-4蛋白与抗体相互作用的机制研究。
2. **文献名称**： "Crystal Structure of the CTLA-4/B7-2 Complex"
**作者**：Stamper, C.C., et al. (2001)
**摘要**：解析了重组CTLA-4胞外域与B7-2配体的晶体结构，揭示了其免疫调节的分子基础，为设计靶向药物提供结构生物学依据。
3. **文献名称**： "Recombinant CTLA-4-Fc Fusion Protein as a Therapeutic Agent in Autoimmunity"
**作者**：Kremer, J.M., et al. (2003)
**摘要**：评估重组CTLA-4-Fc融合蛋白(如Abatacept)在类风湿关节炎中的疗效，证明其通过抑制T细胞共刺激通路缓解自身免疫反应。
提示：建议通过PubMed或Google Scholar搜索关键词 **"recombinant CTLA-4 protein"** 或 **"CTLA-4 immunotherapy"** 获取最新文献。真实文献需核对DOI及发表年限。
**Background of CTL4 Recombinant Protein**
CTL4 (C-type lectin 4) is a calcium-dependent carbohydrate-binding protein belonging to the C-type lectin family, which plays a critical role in innate immune responses across various organisms, particularly in insects. These proteins are characterized by their ability to recognize specific sugar moieties on pathogen surfaces, such as lipopolysaccharides (LPS) in Gram-negative bacteria or β-1.3-glucans in fungi, facilitating pathogen detection and immune activation. In insects like *Bombyx mori* (silkworm), CTL4 is implicated in antimicrobial defense by binding to microbial surfaces, promoting agglutination, and triggering downstream immune pathways like the Toll or IMD signaling cascades.
Structurally, CTL4 contains one or more carbohydrate-recognition domains (CRDs) that mediate interactions with microbial glycans. Recombinant CTL4 is typically produced via heterologous expression systems, such as *E. coli* or insect cell cultures, followed by purification to study its biochemical and functional properties. Research has shown that recombinant CTL4 exhibits broad-spectrum antimicrobial activity *in vitro*, highlighting its potential as a therapeutic agent or agricultural biopesticide.
Studies on CTL4 also explore its role in enhancing host immunity. For instance, overexpression of CTL4 in transgenic insects improves resistance to bacterial infections, suggesting applications in developing disease-resistant silkworm strains for sericulture. Additionally, its pathogen-binding specificity provides insights into host-pathogen co-evolution and novel targets for anti-infective drug design. Further investigations focus on optimizing CTL4 production, understanding its interactions with immune receptors, and evaluating its efficacy in biomedical or biotechnological contexts.
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